Recombinant Anti-P2Y12 antibody [EPR26298-93] (ab300140)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26298-93] to P2Y12
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-P2Y12 antibody [EPR26298-93]
See all P2Y12 primary antibodies -
Description
Rabbit monoclonal [EPR26298-93] to P2Y12 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Frmore details
Unsuitable for: Flow Cyt,ICC/IF,IP or WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human cerebrum, Mouse cerebrum, Rat cerebrum and Human liver tissues.\nIHC-Fr: Mouse cerebrum and Rat cerebrum tissues.\n
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26298-93 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/40000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
1/500.
|
Notes |
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IHC-P
1/40000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/500. |
Target
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Function
Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Involved in platelets aggregation. -
Tissue specificity
Highly expressed in the platelets, lower levels in the brain. Lowest levels in the lung, appendix, pituitary and adrenal gland. Expressed in the spinal cord and in the fetal brain. -
Involvement in disease
Defects in P2RY12 are the cause of P2RY12 deficiency (P2RY12D) [MIM:609821]. It is a condition characterized by severe impairment of platelet response to ADP and abnormal bleeding marked by excessive posttraumatic and postsurgical blood loss. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 64805 Human
- Entrez Gene: 70839 Mouse
- Entrez Gene: 64803 Rat
- Omim: 600515 Human
- SwissProt: Q9H244 Human
- SwissProt: Q9CPV9 Mouse
- SwissProt: Q9EPX4 Rat
- Unigene: 591281 Human
see all -
Alternative names
- ADP glucose receptor antibody
- ADP-glucose receptor antibody
- ADPG R antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling P2Y12 with ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).
Positive staining on microglial cells in human cerebrum (PMID: 30196821). The section was incubated with ab300140 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling P2Y12 with ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Positive staining on microglial cells in rat cerebrum (PMID: 30196821). The section was incubated with ab300140 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).
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Immunohistochemical analysis of paraffin-embedded human liver tissue labeling P2Y12 with ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Negative control: no staining on human liver. The section was incubated with ab300140 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentSecondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).
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Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling P2Y12 with ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Positive staining on microglial cells in mouse cerebrum (PMID: 30196821). The section was incubated with ab300140 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).
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Immunohistological analysis of 4% PFA fixed and 0.2% Triton X-100 parmeabilized frozen rat cerebrum tissue labeling P2Y12 with ab300140 at 1/500 dilution, followed by (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. DAPI was used as nuclear counterstain.
Positive staining on rat cerebrum.
Secondary antibody only control used PBS instead of primary antibody, followed by secondary (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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Immunohistological analysis of 4% PFA fixed and 0.2% Triton X-100 parmeabilized frozen mouse cerebrum (fresh) tissue labeling P2Y12 with ab300140 at 1/500 dilution, followed by (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. DAPI was used as nuclear counterstain.
Positive staining on mouse cerebrum.
Secondary antibody only control used PBS instead of primary antibody, followed by secondary (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300140 has not yet been referenced specifically in any publications.