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    products/primary-antibodies/p38-alphamapk14-antibody-e229-ab170099.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (165)

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Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
  • Immunocytochemistry/ Immunofluorescence - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
  • Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
  • Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
  • Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
  • Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
  • Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
  • Immunoprecipitation - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
  • Flow Cytometry (Intracellular) - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
  • Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E229] to p38 alpha/MAPK14
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

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Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)
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Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (ab218577)
ELISA
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p38 MAPK alpha (pT180/Y182) ELISA Kit (ab221011)

View more associated products

Overview

  • Product name

    Anti-p38 alpha/MAPK14 antibody [E229]
    See all p38 alpha/MAPK14 primary antibodies
  • Description

    Rabbit monoclonal [E229] to p38 alpha/MAPK14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human p38 aa 150-250 (internal sequence). The exact sequence is proprietary.
    Database link: Q16539

  • Positive control

    • WB: Jurkat, C6, NIH/3T3 or HeLa whole cell lysate (ab150035). ICC/IF: NIH/3T3 cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E229
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • MAPK pathway
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Kits/ Lysates/ Other
    • Kits
    • Cell Signaling Kits
    • Other Cell Signaling Kits

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-p38 alpha/MAPK14 antibody [E229] (ab209763)
    • Alexa Fluor® 488 Anti-p38 alpha/MAPK14 antibody [E229] (ab210450)
    • Alexa Fluor® 594 Anti-p38 alpha/MAPK14 antibody [E229] (ab210646)
    • Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free (ab225534)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human p38 alpha/MAPK14 protein (ab82188)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/40.
WB (3)
1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Notes
Flow Cyt (Intra)
1/40.
WB
1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.

Target

  • Function

    Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additionnal targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis.
  • Tissue specificity

    Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-180 and Tyr-182 by the MAP2Ks MAP2K3/MKK3, MAP2K4/MKK4 and MAP2K6/MKK6 in response to inflammatory citokines, environmental stress or growth factors, which a ctivates the enzyme. Dual phosphorylation can also be mediated by TAB1-mediated autophosphorylation. TCR engagement in T-cells also leads to Tyr-323 phosphorylation by ZAP70. Dephosphorylated and inactivated by DUPS1, DUSP10 and DUSP16.
    Acetylated at Lys-53 and Lys-152 by KAT2B and EP300. Acetylation at Lys-53 increases the affinity for ATP and enhances kinase activity. Lys-53 and Lys-152 are deacetylated by HDAC3.
    Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q16539 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1432 Human
    • Entrez Gene: 26416 Mouse
    • Entrez Gene: 81649 Rat
    • Omim: 600289 Human
    • SwissProt: Q16539 Human
    • SwissProt: P47811 Mouse
    • SwissProt: P70618 Rat
    • Unigene: 485233 Human
    • Alternative names

      • CSAID-binding protein antibody
      • Csaids binding protein antibody
      • CSBP antibody
      • CSBP1 antibody
      • CSBP2 antibody
      • CSPB1 antibody
      • Cytokine suppressive anti-inflammatory drug-binding protein antibody
      • EXIP antibody
      • MAP kinase 14 antibody
      • MAP kinase MXI2 antibody
      • MAP kinase p38 alpha antibody
      • MAPK 14 antibody
      • MAPK14 antibody
      • MAX-interacting protein 2 antibody
      • Mitogen-activated protein kinase 14 antibody
      • Mitogen-activated protein kinase p38 alpha antibody
      • MK14_HUMAN antibody
      • Mxi2 antibody
      • p38 antibody
      • p38 MAP kinase antibody
      • p38 mitogen activated protein kinase antibody
      • p38ALPHA antibody
      • p38alpha Exip antibody
      • PRKM14 antibody
      • PRKM15 antibody
      • RK antibody
      • SAPK2A antibody
      • Stress-activated protein kinase 2a antibody
      see all

    Images

    • Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: p38 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab170099 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab170099 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab170099 and

      ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      Immunocytochemistry/ Immunofluorescence - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

      Immunocytochemistry/ Immunofluorescence analysis of HeLa(Human epithelial cell line from cervix adenocarcinoma) cells labeling p38 with ab170099 at 1/250. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077, an AlexaFluor®488 Goat anti-Rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, an anti-alpha tubulin antibody [DM1A] microtubule marker (Alexa Fluor® 594) at 1/200. Nuclei counterstained with DAPI (blue). 

      Confocal image shows nuclear and cytoplasmic staining on HeLa cell line.

    • Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: p38 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab170099 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.

      This western blot image is a comparison between ab170099 and a competitor's top cited rabbit polyclonal antibody.

    • Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      Anti-p38 alpha/MAPK14 antibody [E229] (ab170099) at 1/5000 dilution (purified) + C6 cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 42 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      All lanes : Anti-p38 alpha/MAPK14 antibody [E229] (ab170099) at 1/5000 dilution (purified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 42 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      All lanes : Anti-p38 alpha/MAPK14 antibody [E229] (ab170099) at 1/5000 dilution (purified)

      Lane 1 : NIH/3T3 cell lysate
      Lane 2 : 3T3-L1 cell lysate
      Lane 3 : PC-12 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
      Lane 3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 20 µg

      Predicted band size: 42 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      All lanes : Anti-p38 alpha/MAPK14 antibody [E229] (ab170099) at 1/5000 dilution (purified)

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HEK293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 42 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunoprecipitation - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      Immunoprecipitation - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

      ab170099 (purified) at 1/20 immunoprecipitating p38 in Jurkat whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Flow Cytometry (Intracellular) - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      Flow Cytometry (Intracellular) - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

      Intracellular Flow Cytometry analysis ofHeLa cells labelling p38 with purified ab170099 at 1/40 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

       

       

    • Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)
      Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (165)

    Publishing research using ab170099? Please let us know so that we can cite the reference in this datasheet.

    ab170099 has been referenced in 165 publications.

    • Wu Y  et al. LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration. Cell Res 31:450-462 (2021). PubMed: 32973339
    • Sun P  et al. Hsp90 modulates human sperm capacitation via the Erk1/2 and p38 MAPK signaling pathways. Reprod Biol Endocrinol 19:39 (2021). PubMed: 33663544
    • Bu X  et al. Butylphthalide inhibits nerve cell apoptosis in cerebral infarction rats via the JNK/p38 MAPK signaling pathway. Exp Ther Med 21:565 (2021). PubMed: 33850537
    • Hu S  et al. Radiation-induced abscopal reproductive effect is driven by TNF-a/p38 MAPK/Rac1 axis in Sertoli cells. Theranostics 11:5742-5758 (2021). PubMed: 33897879
    • Liang X & Ju J Matrine inhibits ovarian cancer cell viability and promotes apoptosis by regulating the ERK/JNK signaling pathway via p38MAPK. Oncol Rep 45:N/A (2021). PubMed: 33786627
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-p38 antibody [E229]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (lung)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (12.5% gel)
    Loading amount
    30 µg
    Treatment
    Ovalbumin
    Specification
    lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Pureunhaneul Lee

    Verified customer

    Submitted Dec 31 2019

    Western blot abreview for Anti-p38 antibody [E229]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    UV (100 J/m2)
    Specification
    HeLa
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 12 2018

    Western blot abreview for Anti-p38 antibody [E229]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (whole fish 7-14 days post fertilization)
    Gel Running Conditions
    Reduced Denaturing (12% BioRad)
    Loading amount
    20 µg
    Specification
    whole fish 7-14 days post fertilization
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Jennifer Rahn

    Verified customer

    Submitted Mar 07 2016

    ChIP abreview for Anti-p38 antibody [E229]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Cell lysate - nuclear (MEFs)
    Negative control
    MEFs KO for p38
    Specification
    MEFs
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: formaldehide 1%
    Positive control
    MEFs treated with NaCl for 45 min to recrute p38 to Egr-1 Chromatin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 15 2015

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