Recombinant Anti-P4HB antibody [EPR9498] (ab137119)
![Western blot - Anti-P4HB antibody [EPR9498] (ab137119)](https://www.abcam.com/ps/products/137/ab137119/Images/ab137119-338221-anti-p4hb-antibody-epr9498-western-blot.jpg "Western blot - Anti-P4HB antibody [EPR9498] (ab137119)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9498] to P4HB
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-P4HB antibody [EPR9498]
See all P4HB primary antibodies -
Description
Rabbit monoclonal [EPR9498] to P4HB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: FM,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human P4HB aa 50-150. The exact sequence is proprietary.
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Positive control
- Recombinant Human P4HB protein (ab115719) can be used as a positive control in WB. HeLa, HepG2, A431, and MCF7 cell lysates, Human colon adenocarcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9498 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 5034 Human
- Entrez Gene: 18453 Mouse
- Entrez Gene: 25506 Rat
- Omim: 176790 Human
- SwissProt: P07237 Human
- SwissProt: P09103 Mouse
- SwissProt: P04785 Rat
- Unigene: 464336 Human
see all -
Alternative names
- Cellular thyroid hormone binding protein antibody
- Cellular thyroid hormone-binding protein antibody
- Collagen prolyl 4 hydroxylase beta antibody
see all
Images
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Western blot - Anti-P4HB antibody [EPR9498] (ab137119)All lanes : Anti-P4HB antibody [EPR9498] (ab137119) at 1 µg/ml
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : P4HB knockout HeLa whole cell lysate
Lane 3 : HEP G2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 57 kDaLanes 1 - 3: Merged signal (red and green). Green - ab137119 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab137119 was shown to specifically react with P4HB (Protein disulfide-isomerase) in wild-type HeLa cells as signal was lost in P4HB knockout cells. Wild-type and P4HB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab137119 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-P4HB antibody [EPR9498] (ab137119)](https://www.abcam.com/ps/products/137/ab137119/Images/ab137119-151741-anti-p4hb-antibody-epr9498-western-blot.jpg)
Western blot - Anti-P4HB antibody [EPR9498] (ab137119)All lanes : Anti-P4HB antibody [EPR9498] (ab137119) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [EPR9498] (ab137119)](https://www.abcam.com/ps/products/137/ab137119/Images/ab137119-151740-anti-p4hb-antibody-epr9498-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [EPR9498] (ab137119)Immunohistochemical analysis of paraffin embedded Human colon adenocarcinoma tissue labelling P4HB with ab137119 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-P4HB antibody [EPR9498] (ab137119)](https://www.abcam.com/ps/products/137/ab137119/Images/ab137119-4-benefits-of-recombinant-antibodies.png)
Anti-P4HB antibody [EPR9498] (ab137119)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137119 has been referenced in 2 publications.
- Yang W et al. Collagen Type X Alpha 1 (COL10A1) Contributes to Cell Proliferation, Migration, and Invasion by Targeting Prolyl 4-Hydroxylase Beta Polypeptide (P4HB) in Breast Cancer. Med Sci Monit 27:e928919 (2021). PubMed: 33637669
- Sahebekhtiari N et al. Deficiency of the mitochondrial sulfide regulator ETHE1 disturbs cell growth, glutathione level and causes proteome alterations outside mitochondria. Biochim Biophys Acta Mol Basis Dis 1865:126-135 (2019). PubMed: 30391543