Name: Anti-p73 antibody [EPR18409(T)(MIX)]
Brand: RabMAb
SKU: ab189896
Rating: 4 (3 reviews)

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Western blot - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18409(T)(MIX)] to p73
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-p73 antibody [EPR18409(T)(MIX)]
See all p73 primary antibodies
Description

Rabbit monoclonal [EPR18409(T)(MIX)] to p73
Host species

Rabbit
Specificity

The immunogen used for this product shares 76% homology with p63. Cross-reactivity with this protein has not been confirmed experimentally.
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Recombinant fragment of human p73 protein; HeLa, Jurkat, K562, WEHI-3, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain and fetal kidney lysates; Mouse, brain, heart, kidney and spleen lysates; Rat brain and spleen lysates. IHC-P: Human testis, glioma and gastric carcinoma tissues. ICC/IF: MCF7 and HeLa cells. Flow Cyt (intra): Jurkat and K562 cells. IP: HeLa and MCF7 whole cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18409(T)(MIX)
Isotype

IgG
Research areas

Alternative Versions
- Anti-p73 antibody [EPR18409(T)(MIX)] - BSA and Azide free (ab250999)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab189896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/300.
WB	(1)	1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P	(1)	1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF	(1)	1/1000.
IP		1/80.

Notes
Flow Cyt (Intra) 1/300.
WB 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/1000.
IP 1/80.

Function

Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
Tissue specificity

Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
Sequence similarities

Belongs to the p53 family.
Contains 1 SAM (sterile alpha motif) domain.
Domain

Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
The WW-binding motif mediates interaction with WWOX.
Post-translational
modifications

Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
Ubiquitinated; leading to its degradation by the proteasome.
Cellular localization

Nucleus. Accumulates in the nucleus in response to DNA damage.
Target information above from: UniProt accession O15350 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7161 Human
- Entrez Gene: 22062 Mouse
- Entrez Gene: 362675 Rat
- Omim: 601990 Human
- SwissProt: O15350 Human
- SwissProt: Q9JJP2 Mouse
- Unigene: 697294 Human
- Unigene: 706990 Human
- Unigene: 78015 Mouse
see all
Alternative names
- p53 like transcription factor antibody
- p53 related protein antibody
- p53-like transcription factor antibody
- p53-related protein antibody
- p73 antibody
- P73_HUMAN antibody
- TP73 antibody
- Tumor protein p73 antibody
see all

Western blot - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896) at 1/5000 dilution + Recombinant fragment of human p73 protein at 0.005 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 70 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Recombinant fragment of human p73 protein contains aa380-636 with His-Tag®.
Western blot - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

All lanes : Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896) at 1/5000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : WEHI-3 (Mouse leukemia cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

All lanes : Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896) at 1/5000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

All lanes : Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896) at 1/5000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

All lanes : Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896) at 1/5000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling p73 alpha+beta with ab189896 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on Human cerebral cortex.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling p73 alpha+beta with ab189896 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic and weak nucleus staining on Human spermatogonial cells is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling p73 alpha+beta with ab189896 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus and cytoplasmic staining on Human glioma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling p73 alpha+beta with ab189896 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus and cytoplasmic staining on Human gastric carcinoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling p73 alpha+beta with ab189896 at 1/1000 dilution, followed by Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear and cytoplasm staining on MCF7 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab189896 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling p73 alpha+beta with ab189896 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic and weakly nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab189896 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling p73 alpha+beta with ab189896 at 1/300 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling p73 alpha+betawith ab189896 at 1/300 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

p73 alpha+beta was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab189896 at 1/80 dilution.
Western blot was performed from the immunoprecipitate using ab189896 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: HeLa whole cell lysate 10ug (Input).
Lane 2: ab189896 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189896 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Immunoprecipitation - Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

p73 alpha+beta was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab189896 at 1/80 dilution.
Western blot was performed from the immunoprecipitate using ab189896 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: MCF7 whole cell lysate 10ug (Input).
Lane 2: ab189896 IP in MCF7 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189896 in MCF7 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab189896? Please let us know so that we can cite the reference in this datasheet.

ab189896 has been referenced in 2 publications.

Iannolo G et al. Zika virus infection induces MiR34c expression in glioblastoma stem cells: new perspectives for brain tumor treatments. Cell Death Dis 10:263 (2019). PubMed: 30890698
El Husseini N & Hales BF The Roles of P53 and Its Family Proteins, P63 and P73, in the DNA Damage Stress Response in Organogenesis-Stage Mouse Embryos. Toxicol Sci 162:439-449 (2018). PubMed: 29228353

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1-3 of 3 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (MCF7 (breast cancer), HepG2 (liver cancer), 293T ()

Gel Running Conditions

Reduced Denaturing (10% SDS-PAGE)

Loading amount

12 µg

Specification

MCF7 (breast cancer), HepG2 (liver cancer), 293T (

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 01 2018

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (MDA-MB-231)

Permeabilization

Yes - Tween-20

Specification

MDA-MB-231

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C

Fixative

Acetone

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 09 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (paraffin-embedded human lung carcinoma)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid

Permeabilization

Yes - Tween-20

Specification

paraffin-embedded human lung carcinoma

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 09 2018

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (2)

Customer reviews and Q&As

Western blot abreview for Anti-p73 antibody [EPR18409(T)(MIX)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-p73 antibody [EPR18409(T)(MIX)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p73 antibody [EPR18409(T)(MIX)]

Post-translational
modifications