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RecombinantRabMAb

Recombinant Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)

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Western blot - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)
  • Immunoprecipitation - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)
  • Dot Blot - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)
  • Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21751] to p73 (phospho Y99)
  • Suitable for: Dot blot, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-p73 (phospho Y99) antibody [EPR21751]
    See all p73 primary antibodies

  • Description

    Rabbit monoclonal [EPR21751] to p73 (phospho Y99)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HT-1376 treated with 1 mM pervanadate for 30 minutes, whole cell lysate. IP: HT-1376 treated with 1 mM pervanadate for 30 minutes, whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab218625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
1/1000.
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
IP
1/30.
Notes
Dot blot
1/1000.
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
IP
1/30.

Target

  • Function

    Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.

  • Tissue specificity

    Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.

  • Sequence similarities

    Belongs to the p53 family.
    Contains 1 SAM (sterile alpha motif) domain.

  • Domain

    Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
    The WW-binding motif mediates interaction with WWOX.

  • Post-translational
    modifications

    Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
    Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
    Ubiquitinated; leading to its degradation by the proteasome.

  • Cellular localization

    Nucleus. Accumulates in the nucleus in response to DNA damage.
  • Information by UniProt

  • Database links

    • Alternative names

      • p53 like transcription factor antibody
      • p53 related protein antibody
      • p53-like transcription factor antibody
      • p53-related protein antibody
      • p73 antibody
      • P73_HUMAN antibody
      • TP73 antibody
      • Tumor protein p73 antibody
      see all

    Images

    • Western blot - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)
      Western blot - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)
      All lanes : Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625) at 1/1000 dilution

      Lane 1 : Untreated HT-1376 (human urinary bladder carcinoma cell line) whole cell lysate
      Lane 2 : HT-1376 treated with 1 mM pervanadate for 30 minutes, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 69 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular mass observed is consistent with the literature (PMID:11466610).

    • Immunoprecipitation - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)
      Immunoprecipitation - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)

      p73 (phospho Y99) was immunoprecipitated from 0.35 mg of HT-1376 (human urinary bladder carcinoma cell line) treated with 1mM Pervanadate for 30 min whole cell lysate with ab218625 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218625 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.

      Lane 1: HT-1376 treated with 1mM Pervanadate for 30 min whole cell lysate 10 μg (input).

      Lane 2: ab218625 IP in HT-1376 treated with 1mM Pervanadate for 30 min whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218625 in HT-1376 treated with 1mM Pervanadate for 30 min whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 seconds.

    • Dot Blot - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)
      Dot Blot - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)

      Dot blot analysis of p73 (phospho Y99) with ab218625 at 1/1000 dilution.

      Lane 1: p73 (phospho Y99) peptide.

      Lane 2: p73 non-phospho peptide.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)
      Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab218625? Please let us know so that we can cite the reference in this datasheet.

    ab218625 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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