Recombinant Anti-p95/NBS1 Antibody [RP23040230] (ab313492)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [RP23040230] to p95/NBS1
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Rat, Human
Overview
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Product name
Anti-p95/NBS1 Antibody [RP23040230]
See all p95/NBS1 primary antibodies -
Description
Rabbit recombinant multiclonal [RP23040230] to p95/NBS1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human p95/NBS1 aa 400-800. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60934 -
Positive control
- ICC/IF: A549 cells WB: A549 cell lysate, HeLa cell lysate, LNCap cell lysate, MCF7 cell lysate, K562 cell lysate, A431 cell lysate, PC12 cell lysate
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
RP23040230 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab313492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 2 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 85 kDa.
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Notes |
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ICC/IF
Use a concentration of 2 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 85 kDa. |
Target
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Function
Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex. -
Tissue specificity
Ubiquitous. Expressed at high levels in testis. -
Involvement in disease
Nijmegen breakage syndrome
Breast cancer
Aplastic anemia
Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). -
Sequence similarities
Contains 1 BRCT domain.
Contains 1 FHA domain. -
Domain
The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response. -
Post-translational
modificationsPhosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance. -
Cellular localization
Nucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
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Database links
- Entrez Gene: 4683 Human
- Entrez Gene: 85482 Rat
- Omim: 602667 Human
- SwissProt: O60934 Human
- SwissProt: Q9JIL9 Rat
- Unigene: 492208 Human
- Unigene: 25214 Rat
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Alternative names
- AT V1 antibody
- AT V2 antibody
- ATV antibody
see all
Images
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All lanes : Anti-p95/NBS1 Antibody [RP23040230] (ab313492) at 1 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : LNCap cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : A431 cell lysate
Lane 7 : PC12 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa -
All lanes : Anti-p95/NBS1 Antibody [RP23040230] (ab313492) at 1 µg/ml
Lane 1 : Wild type HeLa cell lysate
Lane 2 : HeLa CAS9 cell lysate
Lane 3 : NBN KO HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate
Predicted band size: 85 kDa
Observed band size: 85 kDa -
All lanes : Anti-p95/NBS1 Antibody [RP23040230] (ab313492) at 1 µg/ml
Lane 1 : Untransfected HeLa cell lysate
Lane 2 : non-specific scrambled siRNA transfected HeLa cell lysate
Lane 3 : NBS1 specific siRNA transfected HeLa cell lysate
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa -
Immunofluorescent analysis of A549 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous NBS1 with ab313492 at 2 µg/mL dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of NBS1 (Nibrin)/NBN protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating Nuclear localization of NBS1 (Nibrin)/NBN. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab313492 has not yet been referenced specifically in any publications.