Anti-PABPN1 antibody [EP3001Y] (ab81224)
![Western blot - Anti-PABPN1 antibody [EP3001Y] (ab81224)](https://www.abcam.com/ps/products/81/ab81224/Images/PABP2-Primary-antibodies-ab81224-3.jpg "Western blot - Anti-PABPN1 antibody [EP3001Y] (ab81224)")
Key features and details
- Rabbit monoclonal [EP3001Y] to PABPN1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-PABPN1 antibody [EP3001Y]
See all PABPN1 primary antibodies -
Description
Rabbit monoclonal [EP3001Y] to PABPN1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: T47D, SKBR3, Ramos and Raji cell lysates; IHC: urinary bladder transitional cell carcinoma. Flow Cyt: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP3001Y -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/60.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
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Flow Cyt (Intra)
1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/10000 - 1/50000. Predicted molecular weight: 33 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in PABPN1 are the cause of oculopharyngeal muscular dystrophy (OPMD) [MIM:164300]. OPMD is a form of late-onset slowly progressive myopathy characterized by eyelid ptosis, dysphagia and, sometimes by other cranial and limb-muscle involvement. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Domain
The RRM domain is essential for specific adenine bases recognition in the poly(A) tail but not sufficient for poly(A) binding. -
Post-translational
modificationsArginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus. Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP-sensitive import mechanism. Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Nucleus; nuclear speckle. Colocalizes with SKIP and poly(A) RNA in nuclear speckles. - Information by UniProt
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Database links
- Entrez Gene: 282298 Cow
- Entrez Gene: 8106 Human
- Entrez Gene: 54196 Mouse
- Omim: 602279 Human
- SwissProt: Q28165 Cow
- SwissProt: Q86U42 Human
- SwissProt: Q8CCS6 Mouse
- Unigene: 707712 Human
see all -
Alternative names
- Nuclear poly(A)-binding protein 1 antibody
- OPMD antibody
- PAB2 antibody
see all
Images
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Western blot - Anti-PABPN1 antibody [EP3001Y] (ab81224)All lanes : Anti-PABPN1 antibody [EP3001Y] (ab81224) at 1/20000 dilution
Lane 1 : T47D cell lysates
Lane 2 : SKBR3 cell lysates
Lane 3 : Ramos cell lysates
Lane 4 : Raji cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody [EP3001Y] (ab81224)](https://www.abcam.com/ps/products/81/ab81224/Images/PABP2-Primary-antibodies-ab81224-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody [EP3001Y] (ab81224)Immunohistochemistry staining of paraffin-embedded urinary bladder transitional cell carcinoma tissue, using 1/100 ab81224
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-PABPN1 antibody [EP3001Y] (ab81224)](https://www.abcam.com/ps/products/81/ab81224/Images/PABP2-Primary-antibodies-ab81224-5.jpg)
Flow Cytometry (Intracellular) - Anti-PABPN1 antibody [EP3001Y] (ab81224)Overlay histogram showing HeLa cells stained with ab81224 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81224, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab81224 has been referenced in 2 publications.
- Wheeler JM et al. Activity of the poly(A) binding protein MSUT2 determines susceptibility to pathological tau in the mammalian brain. Sci Transl Med 11:N/A (2019). PubMed: 31852801
- Kim SH et al. ALS-associated proteins TDP-43 and FUS/TLS function in a common biochemical complex to coregulate HDAC6 mRNA. J Biol Chem : (2010). WB ; Human . PubMed: 20720006