Name: Anti-PADI4 / PAD4 antibody [EPR20706]
Brand: RabMAb
SKU: ab214810
Rating: 5 (1 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20706] to PADI4 / PAD4
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat

Carrier Free

Product name

Anti-PADI4 / PAD4 antibody [EPR20706]
See all PADI4 / PAD4 primary antibodies
Description

Rabbit monoclonal [EPR20706] to PADI4 / PAD4
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Mouse spleen tissue; Rat lung tissue. WB: Mouse embryo lysate; ES-D3 whole cell lysate; HEK-293T transfected with mouse PADI4 / PAD4 expression vector containing a myc-His-tag®, whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20706
Isotype

IgG
Research areas

Alternative Versions
- Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab214810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
WB 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Application notes

Is unsuitable for IP.

Function

Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
Tissue specificity

Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
Involvement in disease

Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
Sequence similarities

Belongs to the protein arginine deiminase family.
Cellular localization

Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
Target information above from: UniProt accession Q9UM07 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 18602 Mouse
- Entrez Gene: 29512 Rat
- SwissProt: Q9Z183 Mouse
- SwissProt: O88807 Rat
- Unigene: 250358 Mouse
- Unigene: 11155 Rat
Alternative names
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
- PAD 4 antibody
- PAD antibody
- PADI 4 antibody
- PADI 5 antibody
- PADI H protein antibody
- Padi4 antibody
- PADI4_HUMAN antibody
- PADI5 antibody
- PDI 4 antibody
- PDI 5 antibody
- PDI4 antibody
- PDI5 antibody
- Peptidyl arginine deiminase type IV antibody
- Peptidyl arginine deiminase type V antibody
- Peptidylarginine deiminase IV antibody
- Protein arginine deiminase antibody
- Protein arginine deiminase type 4 antibody
- Protein arginine deiminase type IV antibody
- Protein-arginine deiminase type IV antibody
- Protein-arginine deiminase type-4 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on granulocytes in rat lung.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on granulocytes in mouse spleen.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on granulocytes in mouse lung.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strongly nuclear staining on peripheral blood granulocytes of mouse spleen (PMID: 20733033, PMID: 28281906, PMID: 12393868, PMID: 12833157). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810)This image is courtesy of an abreview by Maria Christophorou

All lanes : Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution

Lane 1 : Wild-type mouse embryonic stem cells (E14) with BSA
Lanes 2-3 : mouse embryonic stem cells (E14) PADI4 Knock out with BSA

Lysates/proteins at 40 µg per lane.

Blocking peptides at 5 % per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Developed using the ECL technique.

Performed under non-reducing conditions.

Predicted band size: 74 kDa

Exposure time: 18 hours
See Abreview
Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810)

All lanes : Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with mouse PADI4 / PAD4 expression vector containing a myc-His-tag®, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 74 kDa
Observed band size: 74 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810)

All lanes : Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution

Lane 1 : Mouse embryo lysate
Lane 2 : ES-D3 (mouse embryonic multipotent stem cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 74 kDa
Observed band size: 74 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab214810? Please let us know so that we can cite the reference in this datasheet.

ab214810 has been referenced in 14 publications.

Young C et al. Intrinsic function of the peptidylarginine deiminase PADI4 is dispensable for normal haematopoiesis. Biol Open 11:N/A (2022). WB ; Mouse . PubMed: 35603697
Sams KL et al. Delayed puberty, gonadotropin abnormalities and subfertility in male Padi2/Padi4 double knockout mice. Reprod Biol Endocrinol 20:150 (2022). WB ; Mouse . PubMed: 36224627
Chi H et al. A proteomic approach towards understanding the pathogenesis of Mooren's ulcer. Exp Eye Res N/A:108509 (2021). WB, IHC-P ; Human . PubMed: 33647271
Liu Y et al. Tetramethylpyrazine inhibits neutrophil extracellular traps formation and alleviates hepatic ischemia/reperfusion injury in rat liver transplantation. Exp Cell Res N/A:112719 (2021). WB ; Rat . PubMed: 34273405
Takishita Y et al. Formation of neutrophil extracellular traps in mitochondrial DNA-deficient cells. J Clin Biochem Nutr 66:15-23 (2020). WB ; Human . PubMed: 32001952

View all Publications for this product

Customer reviews and Q&As

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Application

Western blot

Sample

Mouse Cell lysate - whole cell (mouse embryonic stem cells (E14))

Gel Running Conditions

Non-reduced Denaturing (4-20% gradient gel)

Loading amount

40 µg

Specification

mouse embryonic stem cells (E14)

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Maria Christophorou

Verified customer

Submitted Jan 09 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (14)

Customer reviews and Q&As

Western blot abreview for Anti-PADI4 / PAD4 antibody [EPR20706]