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Anti-PAI1 antibody [1D5] (ab125687)

Submit a review Q&A (3)References (12)
ELISA - Anti-PAI1 antibody [1D5] (ab125687)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)
  • Flow Cytometry - Anti-PAI1 antibody [1D5] (ab125687)

Key features and details

  • Mouse monoclonal [1D5] to PAI1
  • Suitable for: ELISA, Flow Cyt, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PAI1 antibody [1D5]
    See all PAI1 primary antibodies

  • Description

    Mouse monoclonal [1D5] to PAI1

  • Host species

    Mouse

  • Tested applications

    Suitable for: ELISA, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Purified recombinant fragment of Human PAI1 expressed in E. Coli.

  • Positive control

    • IHC-P: Human lung and kidney cancer tissue. Flow Cyt: NIH 3T3 cells.

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR201079-27 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/10000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
1/200 - 1/1000.
Notes
ELISA
1/10000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
1/200 - 1/1000.

Target

  • Function

    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.

  • Tissue specificity

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

  • Involvement in disease

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.

  • Sequence similarities

    Belongs to the serpin family.

  • Post-translational
    modifications

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Clade E antibody
      • Endothelial plasminogen activator inhibitor antibody
      • Nexin antibody
      • Nexin plasminogen activator inhibitor type 1 antibody
      • PAI 1 antibody
      • PAI antibody
      • PAI-1 antibody
      • PAI1_HUMAN antibody
      • PLANH1 antibody
      • Plasminogen activator inhibitor 1 antibody
      • Plasminogen activator inhibitor type 1 antibody
      • Serine (or cysteine) proteinase inhibitor antibody
      • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
      • Serine proteinase inhibitor clade E member 1 antibody
      • serpin antibody
      • Serpin E1 antibody
      • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
      • Serpin peptidase inhibitor clade E antibody
      • Serpine 1 antibody
      • SERPINE1 antibody
      see all

    Images

    • ELISA - Anti-PAI1 antibody [1D5] (ab125687)
      ELISA - Anti-PAI1 antibody [1D5] (ab125687)
      ELISA using ab125687.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)
      ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human lung cancer tissue by Immunohistochemistry followed by DAB staining.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)
      ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human kidney cancer tissue by Immunohistochemistry followed by DAB staining.
    • Flow Cytometry - Anti-PAI1 antibody [1D5] (ab125687)
      Flow Cytometry - Anti-PAI1 antibody [1D5] (ab125687)
      ab125687 at 1/200 dilution staining PAI1 in NIH 3T3 cells by Flow Cytometry (green), negative control (red).

    References (12)

    Publishing research using ab125687? Please let us know so that we can cite the reference in this datasheet.

    ab125687 has been referenced in 12 publications.

    • Kong HJ  et al. Crosstalk between YAP and TGFß regulates SERPINE1 expression in mesenchymal lung cancer cells. Int J Oncol 58:111-121 (2021). PubMed: 33367928
    • Belghasem M  et al. Metabolites in a mouse cancer model enhance venous thrombogenicity through the aryl hydrocarbon receptor-tissue factor axis. Blood 134:2399-2413 (2019). PubMed: 31877217
    • Li Y  et al. PTPN2 improved renal injury and fibrosis by suppressing STAT-induced inflammation in early diabetic nephropathy. J Cell Mol Med 23:4179-4195 (2019). PubMed: 30955247
    • Li X  et al. Encapsulated papillary carcinoma of the breast: A clinicopathological study of 49 cases. Curr Probl Cancer N/A:N/A (2018). PubMed: 29731165
    • Peterle GT  et al. PAI-1, CAIX, and VEGFA expressions as prognosis markers in oral squamous cell carcinoma. J Oral Pathol Med 47:566-574 (2018). PubMed: 29693741
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Can you please tell me the transferprice of the following article?

    Anti-PAI1 antibody [1D5] (ab125687)

    Thanks

    Read More
    Answer

    Thank you for contacting us.

    The price for antibody ab125687 is €390 plus €39 shipping charges to Switzerland.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Dear Kate

    We will be using the primary and secondary antibodies for staining formalin fixed, paraffin embedded tissue sections.
    I asked Peter for a discount on the primary antibodies and shipping charge and he also offered the secondary antibody free of charge.

    Regards

    Read More
    Answer

    Thank you for your reply.

    As ab125687 PAI1 antibody [1D5], ab1834 Factor XIIIa antibody [AC-1A1] and ab54488 CD41/Integrin alpha 2b antibody [283.16B7] are all mouse IgG1, Ican suggest to consider the following anti mouse IgG1 secondaries available in our catalog. These are biotin conjugated and have been tested and covered by our guarantee for IHC-Fr. I can recommend to review the online datasheets for further information and ensure they are suitable for your purposes:

    ab98691 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (Biotin), pre-adsorbed
    Biotin conjugated
    Tested in: ELISA, ICC/IF, IHC-P, WB
    Reacts with: Mouse
    https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Biotin-preadsorbed-ab98691.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Biotin-preadsorbed-ab98691.html).

    ab97238 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (Biotin)
    Biotin conjugated ELISA, ICC/IF, IHC-P, WB
    Reacts with: Mouse
    https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Biotin-ab97238.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Biotin-ab97238.html).


    Once you have decided which of these you would like to order, please email my colleague Andrew again (mailto:andrew.richards@abcam.com) with your choice and he will be pleased to place the order for you.

    Please note I am out of the office next week, so I can suggest to contact Andrew if you have any further questions regarding the discounts or the order.

    The scientific support team are happy to help if you have any further technical questions (mailto:technical@abcam.com).

    I hope this provides a satisfactory outcome for you and I wish you a good bank holiday weekend. Good luck with your research.

    Read More

    Question

    Bonjour,

    J’ai bien pris connaissance de votre devis.

    Et je reviens une fois encore vers vous pour quelques questions concernant les anticorps secondaires pour immunohistochimie.

    Je recherche également deux anticorps secondaires couplés à la biotine dirigés contre soit:

    - Des anticorps monoclonaux de souris (pour l’anticorps anti-PAI1 par exemple que je souhaite commander chez vous)

    - L’anticorps polyclonal de lapin (pour l’anticorps anti-PHD3 que je souhaite également commander chez vous).

    J’avoue que je ne comprends pas trop en recherchant sur votre site, si les anticorps secondaires que je choisis sont biotinylés ou non.

    Par exemple, j’ai regardé cet anticorps secondaire:





    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-pre-adsorbed-ab98689.html



    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-pre-adsorbed-ab98689.html


    Les anticorps secondaires pré-adsorbés sont-ils prêt à l’emploi?

    De plus, sera t-il possible d’avoir un geste commercial de votre part pour la commande(le devis) finale ?

    J’attends également qu’une de mes collègues me dise si elle a des choses à commander chez vous.

    Cordialement.

    Read More
    Answer

    Merci de votre question et votre intérêt pour nos produits.

    L'anticorps secondaire ab98689 n'est pas couplé à la biotine - c'est juste l'anticorps secondaire non-conjugué. Les anticorps pré-adsorbés sont des anticorps pour lesquels la réactivité contre des immunoglobulines des espèces homologues avait été enlevé. Ces anticorps sont prêt à l'emploi - ils doivent toute fois toujours être dilués. Le grand avantage est pour ces anticorps qu'ils vont avoir une crossreactivité minimale avec des autres Ig et sont donc très utiles pour diminuer le bruit de fond ou pour des doubles ou multiplesmarquages. Le ab98689 est préabsorbé contre les protéines humaines et de rat comme indiqué sur la fiche technique sous "minimal crossreactivity".

    Comme anticorps secondaire biotinylé contre les Ig ou les IgG1 de souris, je peux vous recommander les anticorps suivants:

    -le ab97033goat to mouse IgG H&L, preabsorbed, Biotin conjugated - Cet anticorps va reconnaitre la chaine lourde et la chaine légère (H&L) des immunoglobulines de souris, et il avait été préabsorbé contre les protéines de poulot, bovine, chèvre, cheval, humain, lapin, rat et mouton https://www.abcam.com/Goat-Mouse-IgG-HL-Biotin-preadsorbed-ab97033.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-Biotin-preadsorbed-ab97033.html).

    -ab97021, goat to mouse IgG H&L, preabsorbed, Biotin conjugated -Cet anticorps n'avais pas été préabsorbé. https://www.abcam.com/Goat-Mouse-IgG-HL-Biotin-ab97021.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-Biotin-ab97021.html).

    Si vous préférez un anticorps contre l'IgG1, je peux vous recommander le ab98691, un goat anti mouse IgG1, Biotin, préabsorbed https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Biotin-preadsorbed-ab98691.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Biotin-preadsorbed-ab98691.html).

    Pour l'anticorps secondaire contre les Ig de lapin, je peux vous recommanderl'anticorpssuivant:

    -ab98497 donkeytorabbit IgG H&L, preabsorbed, Biotin conjugated - Cet anticorps va reconnaitre la chaine lourde et la chaine légère (H&L) des immunoglobulines de lapin, et il avait été préabsorbé contre les protéines humaines, de souris et de rat https://www.abcam.com/Donkey-Rabbit-IgG-HL-Biotin-preadsorbed-ab98497.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-Biotin-preadsorbed-ab98497.html).

    Ma collègue va vous recontacter avec un nouveau devis - incluant le ab97033 et le ab98497. Veuillez ne pas hésiter de nous recontacter si vous préférez des autres anticorps secondaires et si vous avez des autres produits à ajouter sur le devis.

    J'espère que ces informations vous sont utiles. N'hésitez pas à nous recontacter si vous avez besoin d'autres renseignements ou conseils.

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