Anti-PAI1 antibody (ab28207)
Key features and details
- Rabbit polyclonal to PAI1
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PAI1 antibody
See all PAI1 primary antibodies -
Description
Rabbit polyclonal to PAI1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length PAI1 protein (Mouse).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.60
Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This is an IgG purified fraction (purified by Protein A Sepharose). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration. PubMed: 18276785
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| Notes |
|---|
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WB
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 18276785 |
Target
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Function
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
Tissue specificity
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
Involvement in disease
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsInactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 18787 Mouse
- SwissProt: P22777 Mouse
- Unigene: 250422 Mouse
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Alternative names
- Clade E antibody
- Endothelial plasminogen activator inhibitor antibody
- Nexin antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (18)
ab28207 has been referenced in 18 publications.
- Cohen C et al. Glomerular endothelial cell senescence drives age-related kidney disease through PAI-1. EMBO Mol Med 13:e14146 (2021). PubMed: 34725920
- Wu X et al. MicroRNA-663 participates in myocardial fibrosis through interaction with TGF-ß1. Exp Ther Med 18:3172-3176 (2019). PubMed: 31572558
- Girard RA et al. Increased expression of plasminogen activator inhibitor-1 (PAI-1) is associated with depression and depressive phenotype in C57Bl/6J mice. Exp Brain Res 237:3419-3430 (2019). PubMed: 31734788
- Burchall GF et al. Expression of the plasminogen system in the physiological mouse ovary and in the pathological polycystic ovary syndrome (PCOS) state. Reprod Biol Endocrinol 17:33 (2019). PubMed: 30878040
- Bennett RE et al. Tau induces blood vessel abnormalities and angiogenesis-related gene expression in P301L transgenic mice and human Alzheimer's disease. Proc Natl Acad Sci U S A 115:E1289-E1298 (2018). PubMed: 29358399
