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  1. Link

    products/primary-antibodies/pai1-antibody-ab28207.pdf

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Cardiovascular Blood Fibrinolysis / Thrombolysis
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Anti-PAI1 antibody (ab28207)

  • Datasheet
Submit a review Q&A (1)References (17)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Rabbit polyclonal to PAI1
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-PAI1 antibody [EPR17795] (ab187262)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-PAI1 antibody
    See all PAI1 primary antibodies
  • Description

    Rabbit polyclonal to PAI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length PAI1 protein (Mouse).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.60
    Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    This is an IgG purified fraction (purified by Protein A Sepharose).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Serine protease inhibitors
    • SERPINs
    • Cancer
    • Tumor biomarkers
    • Other
    • Metabolism
    • Types of disease
    • Metabolic disorders

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant mouse PAI1 protein (ab93068)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab28207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration. PubMed: 18276785
Notes
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration. PubMed: 18276785

Target

  • Function

    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificity

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in disease

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P05121 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 18787 Mouse
    • SwissProt: P22777 Mouse
    • Unigene: 250422 Mouse
    • Alternative names

      • Clade E antibody
      • Endothelial plasminogen activator inhibitor antibody
      • Nexin antibody
      • Nexin plasminogen activator inhibitor type 1 antibody
      • PAI 1 antibody
      • PAI antibody
      • PAI-1 antibody
      • PAI1_HUMAN antibody
      • PLANH1 antibody
      • Plasminogen activator inhibitor 1 antibody
      • Plasminogen activator inhibitor type 1 antibody
      • Serine (or cysteine) proteinase inhibitor antibody
      • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
      • Serine proteinase inhibitor clade E member 1 antibody
      • serpin antibody
      • Serpin E1 antibody
      • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
      • Serpin peptidase inhibitor clade E antibody
      • Serpine 1 antibody
      • SERPINE1 antibody
      see all

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (17)

    Publishing research using ab28207? Please let us know so that we can cite the reference in this datasheet.

    ab28207 has been referenced in 17 publications.

    • Burchall GF  et al. Expression of the plasminogen system in the physiological mouse ovary and in the pathological polycystic ovary syndrome (PCOS) state. Reprod Biol Endocrinol 17:33 (2019). PubMed: 30878040
    • Girard RA  et al. Increased expression of plasminogen activator inhibitor-1 (PAI-1) is associated with depression and depressive phenotype in C57Bl/6J mice. Exp Brain Res 237:3419-3430 (2019). PubMed: 31734788
    • Wu X  et al. MicroRNA-663 participates in myocardial fibrosis through interaction with TGF-ß1. Exp Ther Med 18:3172-3176 (2019). PubMed: 31572558
    • Bennett RE  et al. Tau induces blood vessel abnormalities and angiogenesis-related gene expression in P301L transgenic mice and human Alzheimer's disease. Proc Natl Acad Sci U S A 115:E1289-E1298 (2018). PubMed: 29358399
    • Magnussen SN  et al. Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - ß1 (TGF-ß1) and potential effects on migration and invasion. BMC Cancer 17:350 (2017). PubMed: 28526008
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Hello,
    We have recently ordered a PAI-1 antibody from abcam and have been very unhappy with the results we are getting. Attached is a western blot image of our gel.
    Here are experimental details:
    -Jurkat cell lysates used at 10ug (have been shown by other westerns to have high levels of PAI-1)
    -4-12% gradient NuPage SDS gel

    -MagicMark™ Western Protein Standard(Cat. no. LC5600)

    -1.2ug/ml PAI-1 antibody pre-absorbed in bio-rad blocking buffer.

    -Secondary Bio-Rad HRP conjugated goat anti-rabbit IgG



    If you need any other experimental details I can provide them, these were the ones I figured would be important.



    We have done several different concentrations of antibody, different blocking times, different incubation times with primary, different exposure times but still we do not see a band around 45kDA, instead we are getting several non-specific bands and a band that is lighting up around 15kDA. For the marker on the gel in the image the two darker bands are 40kDA and 20kDA.



    Please let me know what can be done, as it is frustrating to purchase an antibody and then have it not work.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 16 2012

    Answer

    Thanks for your enquiry and for providing the protocol and data.

    To our knowledge, this antibody has not previously been tested for reactivity to human PAI-1. It may be there is no reactivity to the human protein. Do you happen to have any mouse samples to test for PAI-1 in western blotting to confirm this vial of ab28207 is working as expected?

    Read More

    Abcam Scientific Support

    Answered on Jul 16 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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