Anti-pan Actin Antibody [4A4] (ab119952)
Key features and details
- Mouse monoclonal [4A4] to pan Actin
- Suitable for: WB, ELISA, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-pan Actin Antibody [4A4] -
Description
Mouse monoclonal [4A4] to pan Actin -
Host species
Mouse -
Specificity
Actin proteins have very high homology. The immunogen for this product is based on the ACTA2 protein but this product will also detect Gamma actin, and likely other actin family members too.
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Tested applications
Suitable for: WB, ELISA, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Synthetic peptide corresponding to Human pan Actin aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
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Positive control
- Recombinant alpha smooth muscle Actin protein, Hela, A431, Jurkat, K562, HEK293, HepG2, NIH3T3, PC12 and COS7 cell lysates, HepG2 cells, HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4A4 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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ELISA |
1/10000.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
ELISA
1/10000. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Database links
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Entrez Gene: 81633 Rat
- Omim: 102610 Human
- Omim: 102620 Human
- SwissProt: P62736 Human
- SwissProt: P68032 Human
- SwissProt: P68133 Human
see all -
Alternative names
- a actin antibody
- ACTA antibody
- ACTA1 antibody
see all
Images
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1 ug per mL of recombinant protein ab157838 and ab157841 were immobilised in PBS on an ELISA plate overnight. After blocking in 5% BSA, primary antibody (ab119952) was added in a concentration range of 0.017-1000 ng per mL.
Pre-absorbed secondary antibody goat anti-rabbit IgG H&L (HRP, ab97080) was used at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (ab119952)Immunohistochemical analysis of paraffin-embedded Human duodenum tissue using ab119952 at a dilution of 1/200 with DAB staining.
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All lanes : Anti-pan Actin Antibody [4A4] (ab119952) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : HepG2 cell lysate
Lane 7 : NIH3T3 cell lysate
Lane 8 : PC12 cell lysate
Lane 9 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Predicted band size: 42 kDa
Observed band size: 42 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (ab119952)Immunohistochemical analysis of paraffin-embedded Human esophagus tissue using ab119952 at a dilution of 1/200 with DAB staining.
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Flow cytometric analysis of Hela cells using ab119952 at a dilution of 1/200 (green) and negative control (purple).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab119952 has been referenced in 26 publications.
- Zhou C et al. Anxa1 in smooth muscle cells protects against acute aortic dissection. Cardiovasc Res 118:1564-1582 (2022). PubMed: 33757117
- Tan ML et al. Integrin-β4 regulates the dynamic changes of phenotypic characteristics in association with epithelial-mesenchymal transition (EMT) and RhoA activity in airway epithelial cells during injury and repair. Int J Biol Sci 18:1254-1270 (2022). PubMed: 35173551
- Du P et al. Mucin-fused myeloid-derived growth factor (MYDGF164) exhibits a prolonged serum half-life and alleviates fibrosis in chronic kidney disease. Br J Pharmacol 179:4136-4156 (2022). PubMed: 35393682
- Nishinaka A et al. The pathological association between the anterior eye segment and the retina in a murine model of neovascular glaucoma. FASEB J 36:e22323 (2022). PubMed: 35485981
- Zhao G et al. BAF60c prevents abdominal aortic aneurysm formation through epigenetic control of vascular smooth muscle cell homeostasis. J Clin Invest 132:N/A (2022). PubMed: 36066968