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Anti-pan Actin Antibody [4A4] (ab119952)

Submit a review Q&A (2)References (26)
ELISA - Anti-pan Actin Antibody [4A4] (ab119952)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (ab119952)
  • Western blot - Anti-pan Actin Antibody [4A4] (ab119952)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (ab119952)
  • Flow Cytometry - Anti-pan Actin Antibody [4A4] (ab119952)

Key features and details

  • Mouse monoclonal [4A4] to pan Actin
  • Suitable for: WB, ELISA, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-pan Actin Antibody [4A4]

  • Description

    Mouse monoclonal [4A4] to pan Actin

  • Host species

    Mouse

  • Specificity

    Actin proteins have very high homology. The immunogen for this product is based on the ACTA2 protein but this product will also detect Gamma actin, and likely other actin family members too.

  • Tested applications

    Suitable for: WB, ELISA, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human, African green monkey

  • Immunogen

    Synthetic peptide corresponding to Human pan Actin aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant alpha smooth muscle Actin protein, Hela, A431, Jurkat, K562, HEK293, HepG2, NIH3T3, PC12 and COS7 cell lysates, HepG2 cells, HeLa cells

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab119952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ELISA
1/10000.
IHC-P
1/200 - 1/1000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
WB
1/500 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ELISA
1/10000.
IHC-P
1/200 - 1/1000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Application notes
Is unsuitable for ICC/IF.

Target

Images

  • ELISA - Anti-pan Actin Antibody [4A4] (ab119952)
    ELISA - Anti-pan Actin Antibody [4A4] (ab119952)

    1 ug per mL of recombinant protein ab157838 and ab157841 were immobilised in PBS on an ELISA plate overnight. After blocking in 5% BSA, primary antibody (ab119952) was added in a concentration range of 0.017-1000 ng per mL.

    Pre-absorbed secondary antibody goat anti-rabbit IgG H&L (HRP, ab97080) was used at 1/20000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (ab119952)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (ab119952)
    Immunohistochemical analysis of paraffin-embedded Human duodenum tissue using ab119952 at a dilution of 1/200 with DAB staining.
  • Western blot - Anti-pan Actin Antibody [4A4] (ab119952)
    Western blot - Anti-pan Actin Antibody [4A4] (ab119952)
    All lanes : Anti-pan Actin Antibody [4A4] (ab119952) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : HEK293 cell lysate
    Lane 6 : HepG2 cell lysate
    Lane 7 : NIH3T3 cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (ab119952)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (ab119952)
    Immunohistochemical analysis of paraffin-embedded Human esophagus tissue using ab119952 at a dilution of 1/200 with DAB staining.
  • Flow Cytometry - Anti-pan Actin Antibody [4A4] (ab119952)
    Flow Cytometry - Anti-pan Actin Antibody [4A4] (ab119952)
    Flow cytometric analysis of Hela cells using ab119952 at a dilution of 1/200 (green) and negative control (purple).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (26)

Publishing research using ab119952? Please let us know so that we can cite the reference in this datasheet.

ab119952 has been referenced in 26 publications.

  • Zhou C  et al. Anxa1 in smooth muscle cells protects against acute aortic dissection. Cardiovasc Res 118:1564-1582 (2022). PubMed: 33757117
  • Tan ML  et al. Integrin-β4 regulates the dynamic changes of phenotypic characteristics in association with epithelial-mesenchymal transition (EMT) and RhoA activity in airway epithelial cells during injury and repair. Int J Biol Sci 18:1254-1270 (2022). PubMed: 35173551
  • Du P  et al. Mucin-fused myeloid-derived growth factor (MYDGF164) exhibits a prolonged serum half-life and alleviates fibrosis in chronic kidney disease. Br J Pharmacol 179:4136-4156 (2022). PubMed: 35393682
  • Nishinaka A  et al. The pathological association between the anterior eye segment and the retina in a murine model of neovascular glaucoma. FASEB J 36:e22323 (2022). PubMed: 35485981
  • Zhao G  et al. BAF60c prevents abdominal aortic aneurysm formation through epigenetic control of vascular smooth muscle cell homeostasis. J Clin Invest 132:N/A (2022). PubMed: 36066968
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-2 of 2 Abreviews or Q&A

Question

I am planning to order 3 antibodies (ab10214, ab15, ab119952) from your catalogous to perform flow cytometry with.
These antibodies are made in mouse but I work with murine embryonic stem cells. The data sheet of the 3 antibodies are showing reactivity with mouse.
Can I use it for my murine cells? How can I avoid aspecific binding? ,

Read More
Answer

I can confirm that these three antibodies recognize the mouse protein and the host species in which they were raised is also mouse. This may cause some non-specificity signal problem mainly in immunohistochemical using tissue sections (mouse-on-mouse) due to endogenous mouse immunoglobins. In order to be able to reduce the risk of any high background signal, you may wish to select Fab fragment secondary antibodies - rather than full molecules.

I would recommend visiting this website; You may find some useful information on how to block high background signal:

https://www.abcam.com/index.html?pageconfig=resource&rid=11465

https://docs.abcam.com/pdf/protocols/Mouse-on-Mouse-Protocol.pdf

Read More

Question

In the data sheet belonging to the antibody, you show FACS analysis of HeLa cells. Would it be possible for you to send the information regarding the fixation and permeabilization steps you used in order to produce these nice histograms.

Read More
Answer

The histogram showed in the datasheet was obtained using 4% paraformaldehyde as fixative agent, and 0.1% Triton-X100 and 0.5% BSA in 1X PBS to permeabilise.

Read More

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