Recombinant Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)](https://www.abcam.com/ps/products/86/ab86734/Images/ab86734-232922-anti-pan-cytokeratin-antibody-ae1-ae3-5d3-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse recombinant multiclonal [AE1/AE3 + 5D3] to pan Cytokeratin
- Suitable for: Flow Cyt, ICC/IF, IHC-P
- Reacts with: Goat, Chicken, Dog, Human, Pig
Overview
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Product name
Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3]
See all pan Cytokeratin primary antibodies -
Description
Mouse recombinant multiclonal [AE1/AE3 + 5D3] to pan Cytokeratin -
Host species
Mouse -
Specificity
The ab86734 is a combination of [AE1/AE3] and [5D3] clones and can be used to detect most human epithelia. [AE1/AE3] recognizes acidic and basic subfamilies of cytokeratins, with molecular weights ranging from 40 to 67 kDa. [5D3] recognizes Cytokeratin 8 and 18 intermediate filament proteins. In normal tissues, [5D3] recognizes all simple and glandular epithelium. It has been observed that [AE1/AE3] has had problems marking certain tissues types and adenocarcinomas. The addition of [5D3] may remedy some of the limitations observed when staining with [AE1/AE3] alone.
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Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Goat, Chicken, Dog, Human, Pig -
Immunogen
Full length protein corresponding to pan Cytokeratin.
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Positive control
- Skin or adenocarcinoma This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.
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General notes
This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Buffer with protein carrier -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Recombinant Multiclonal -
Clone number
AE1/AE3 + 5D3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (1) |
1/100.
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| IHC-P | (9) |
Use at an assay dependent concentration.
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| Notes |
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Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF
1/100. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
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Alternative names
- pan ck antibody
- pan-ck antibody
- panck antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)This image is courtesy of an Abreview submitted by Carl Hobbs
ab86734 staining pan Cytokeratin in pig small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/250 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)](https://www.abcam.com/ps/products/86/ab86734/Images/ab86734-171179-anti-pan-cytokeratin-antibody-ae1-ae3-5d3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)ab86734 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86734 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) HeLa, Hek293. HepG2, and MCF-7 cells, also in formaldehyde fixed (4%, 10min) HeLa, Hek293, and MCF-7 cells at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)](https://www.abcam.com/ps/products/86/ab86734/Images/ab86734-282752-anti-pan-cytokeratin-antibody-ae1-ae3-5d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)Mr Carl HobbsFormaldehyde fixed dog lung tissue stained for pan Cytokeratin with ab86734 at a 1/250 dilution. Heat mediated - Buffer/Enzyme Used: Citric acid. 1% BSA used for blocking for 10 minutes at RT. Primary incubation for 2 hours at RT in TBS/BSA/Azide. Secondary: Goat polyclonal conjugated to biotin used at a 1/200 dilution.
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![Flow Cytometry - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)](https://www.abcam.com/ps/products/86/ab86734/Images/ab86734-101800-anti-pan-cytokeratin-antibody-ae1-ae3-5d3-flow-cytometry.jpg)
Flow Cytometry - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)Overlay histogram showing A431 cells stained with ab86734 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86734, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)](https://www.abcam.com/ps/products/86/ab86734/Images/ab86734-282750-anti-pan-cytokeratin-antibody-ae1-ae3-5d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)Mr Carl HobbsFormaldehyde fixed goat lung tissue stained for pan Cytokeratin with ab86734 at a 1/250 dilution. Heat mediated - Buffer/Enzyme Used: Citric acid. 1% BSA used for blocking for 10 minutes at RT. Primary incubation for 2 hours at RT in TBS/BSA/Azide. Secondary: Goat polyclonal conjugated to biotin used at a 1/200 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)](https://www.abcam.com/ps/products/86/ab86734/Images/ab86734-282751-anti-pan-cytokeratin-antibody-ae1-ae3-5d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)Mr Carl HobbsFormaldehyde fixed chicken lung tissue stained for pan Cytokeratin with ab86734 at a 1/200 dilution. Heat mediated - Buffer/Enzyme Used: Citric acid. 1% BSA used for blocking for 10 minutes at RT. Primary incubation for 16 hours at RT in TBS/BSA/Azide. Secondary: Goat polyclonal conjugated to biotin used at a 1/200 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)](https://www.abcam.com/ps/products/86/ab86734/Images/ab86734-101802-anti-pan-cytokeratin-antibody-ae1-ae3-5d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)Skin stained for pan Cytokeratin with ab86734 at a 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)](https://www.abcam.com/ps/products/86/ab86734/Images/ab86734-101801-anti-pan-cytokeratin-antibody-ae1-ae3-5d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)This image is courtesy of an Abreview submitted by Carl Hobbsab8673 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab8673 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (49)
ab86734 has been referenced in 49 publications.
- van Wilpe S et al. Intratumoral T cell depletion following neoadjuvant chemotherapy in patients with muscle-invasive bladder cancer is associated with poor clinical outcome. Cancer Immunol Immunother 72:137-149 (2023). PubMed: 35771253
- Hong C et al. Epimedium brevicornum Maxim. Extract exhibits pigmentation by melanin biosynthesis and melanosome biogenesis/transfer. Front Pharmacol 13:963160 (2022). PubMed: 36249817
- van der Woude LL et al. Tumor microenvironment shows an immunological abscopal effect in patients with NSCLC treated with pembrolizumab-radiotherapy combination. J Immunother Cancer 10:N/A (2022). PubMed: 36252995
- Zeng TT et al. HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Cell Death Dis 13:1026 (2022). PubMed: 36476988
- Ippolito L et al. Lactate Rewires Lipid Metabolism and Sustains a Metabolic-Epigenetic Axis in Prostate Cancer. Cancer Res 82:1267-1282 (2022). PubMed: 35135811