Recombinant Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse recombinant multiclonal [AE1+AE3] to pan Cytokeratin, prediluted
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Dog, Human, Fish, Zebrafish
Overview
-
Product name
Anti-pan Cytokeratin antibody [AE1+AE3], prediluted
See all pan Cytokeratin primary antibodies -
Description
Mouse recombinant multiclonal [AE1+AE3] to pan Cytokeratin, prediluted -
Host species
Mouse -
Specificity
This antibody mixture recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52 kDa. -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Dog, Human, Fish, Zebrafish -
Immunogen
Tissue, cells or virus corresponding to pan Cytokeratin. Human epidermal cytokeratins
-
Positive control
- Skin or adenocarcinoma.
-
General notes
This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Proprietary Preservative -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Recombinant Multiclonal -
Clone number
AE1+AE3 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Unmasking reagent
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| ICC/IF |
Use at an assay dependent concentration. PubMed: 19852942
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 19852942 |
Target
-
Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
-
Alternative names
- pan ck antibody
- pan-ck antibody
- panck antibody
Protocols
Datasheets and documents
-
Datasheet download
References (40)
ab961 has been referenced in 40 publications.
- Park JK et al. Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma. Evid Based Complement Alternat Med 2022:6295910 (2022). PubMed: 36110191
- Yao HJ et al. Three new experimental models of anterior urethral stricture in rabbits. Transl Androl Urol 11:761-772 (2022). PubMed: 35812200
- Yang H & Lan L Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids. Methods Mol Biol 2528:271-276 (2022). PubMed: 35704197
- Shmerling M et al. LY6S, a New IFN-Inducible Human Member of the Ly6a Subfamily Expressed by Spleen Cells and Associated with Inflammation and Viral Resistance. Immunohorizons 6:253-272 (2022). PubMed: 35440514
- Rangel-Huerta E et al. The dynamics of epidermal stratification during post-larval development in zebrafish. Dev Dyn 250:175-190 (2021). PubMed: 32877571
