Recombinant Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

Product name

Anti-pan cytokeratin antibody [EPR28285-45]
See all pan Cytokeratin primary antibodies
Description

Rabbit monoclonal [EPR28285-45] to pan Cytokeratin
Host species

Rabbit
Specificity

Unsuitable for mouse WB.
Tested applications

Suitable for: Dot blot, IHC-P, WBmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A431 and HeLa whole cell lysate. Human liver and colon tissue lysate. Rat colon and skin tissue lysate. IHC-P: Human skin, liver and colon tissue. Human colon adenocarcinoma tissue. Mouse and rat skin. HeLa cell pellet.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR28285-45
Isotype

IgG
Research areas

Alternative Versions
- Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Related Products
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab308262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Dot blot		1/1000.
IHC-P		1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000.

Notes
Dot blot 1/1000.
IHC-P 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000.

Application notes

Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

Relevance

Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
Cellular localization

Cytoplasmic
Database links
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
Alternative names
- pan ck antibody
- pan-ck antibody
- panck antibody

Western blot - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

All lanes : Anti-pan cytokeratin antibody [EPR28285-45] (ab308262) at 1/1000 dilution

Lane 1 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 4 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 5 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 37-60 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression: MOLT-4, Ramos, Raji.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

Exposure time: 10 seconds.
Western blot - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

All lanes : Anti-pan cytokeratin antibody [EPR28285-45] (ab308262) at 1/1000 dilution

Lane 1 : Human liver tissue lysate
Lane 2 : Human colon tissue lysate
Lane 3 : Human skeletal muscle tissue lysate
Lane 4 : Human testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 37-65 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression: skeletal muscle, testis.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

Exposure time: 10 seconds.
Western blot - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

All lanes : Anti-pan cytokeratin antibody [EPR28285-45] (ab308262) at 1/1000 dilution

Lane 1 : Rat colon tissue lysate
Lane 2 : Rat skin tissue lysate
Lane 3 : Rat skeletal muscle tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Observed band size: 37-65 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression: skeletal muscle.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

Exposure time: Lane 1: 8 seconds, lanes 2-3: 15 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Positive staining on human skin (PMID:25411189).

The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Positive staining on human colon.

The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Positive staining on human liver.

The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Positive staining on human adenocarcinoma of colon.

The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Positive staining on mouse skin.

The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Positive staining on rat skin.

The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

Immunohistochemical analysis of paraffin-embedded cell pellets labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Cytoplasmic staining on (A) HeLa cell pellet. No staining on (B) Raji cell pellet.

The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Negative control: No staining on human skeletal muscle.

The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Negative control: No staining on mouse skeletal muscle.

The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Negative control: No staining on rat skeletal muscle.

The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Dot Blot - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

Dot blot analysis of pan cytokeratin using ab308262 at 1:1000 dilution (0.541 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

Exposure time: 180 seconds.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Lane 1: Peptides for KRT5, KRT6, KRT7, KRT8
Lane 2: Peptide for KRT2
Lane 3: Peptide for KRT1
Lane 4: Peptide for KRT3
Lane 5: Peptide for KRT4
Lane 6: Peptide for KRT23
Lane 7: Peptides for KRT10, KRT12, KRT15, KRT18, KRT25, KRT26, KRT28
Lane 8: Peptide for KRT20
Lane 9: Peptide for KRT9
Lane 10: Peptides for KRT14, KRT16, KRT17, KRT19, KRT27
Lane 11: Peptide for KRT24
Anti-pan cytokeratin antibody [EPR28285-45] (ab308262)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Related Products

Applications

The Abpromise guarantee

Target

Relevance

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (0)

Customer reviews and Q&As