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RecombinantRabMAb

Recombinant Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

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Western blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Western blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Western blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Dot Blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
  • Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28285-45] to pan Cytokeratin - BSA and Azide free
  • Suitable for: WB, Dot blot, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free
    See all pan Cytokeratin primary antibodies

  • Description

    Rabbit monoclonal [EPR28285-45] to pan Cytokeratin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Unsuitable for mouse WB.

  • Tested applications

    Suitable for: WB, Dot blot, IHC-Pmore details
    Unsuitable for: Flow Cyt (Intra),ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 and HeLa whole cell lysate. Human liver and colon tissue lysate. Rat colon and skin tissue lysate. IHC-P: Human skin, liver and colon tissue. Human colon adenocarcinoma tissue. Mouse and rat skin. HeLa cell pellet.

  • General notes

    ab308263 is a carrier free version of ab308262.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab308263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

Target

  • Relevance

    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.

  • Cellular localization

    Cytoplasmic

  • Database links

    • Alternative names

      • pan ck antibody
      • pan-ck antibody
      • panck antibody

    Images

    • Western blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Western blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      All lanes : Anti-pan cytokeratin antibody [EPR28285-45] (ab308262) at 1/1000 dilution

      Lane 1 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate
      Lane 4 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
      Lane 5 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 37-60 kDa why is the actual band size different from the predicted?



      This data was produced using ab308262, the same clone but in a different formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: MOLT-4, Ramos, Raji.

      In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

      Exposure time: 10 seconds.

    • Western blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Western blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      All lanes : Anti-pan cytokeratin antibody [EPR28285-45] (ab308262) at 1/1000 dilution

      Lane 1 : Human liver tissue lysate
      Lane 2 : Human colon tissue lysate
      Lane 3 : Human skeletal muscle tissue lysate
      Lane 4 : Human testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 37-65 kDa why is the actual band size different from the predicted?



      This data was produced using ab308262, the same clone but in a different formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: skeletal muscle, testis.

      In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

      Exposure time: 10 seconds.

    • Western blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Western blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      All lanes : Anti-pan cytokeratin antibody [EPR28285-45] (ab308262) at 1/1000 dilution

      Lane 1 : Rat colon tissue lysate
      Lane 2 : Rat skin tissue lysate
      Lane 3 : Rat skeletal muscle tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 37-60 kDa why is the actual band size different from the predicted?



      This data was produced using ab308262, the same clone but in a different formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: skeletal muscle.

      In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

      Exposure time: Lane 1: 8 seconds, lanes 2-3: 15 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

      This data was produced using ab308262, the same clone but in a different formulation.

      Immunohistochemical analysis of paraffin-embedded human skin tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Positive staining on human skin (PMID:25411189).

      The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

      This data was produced using ab308262, the same clone but in a different formulation.

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Positive staining on human colon.

      The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

      This data was produced using ab308262, the same clone but in a different formulation.

      Immunohistochemical analysis of paraffin-embedded human liver tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Positive staining on human liver.

      The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

      This data was produced using ab308262, the same clone but in a different formulation.

      Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Positive staining on human adenocarcinoma of colon.

      The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

      This data was produced using ab308262, the same clone but in a different formulation.

      Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Positive staining on mouse skin.

      The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

      This data was produced using ab308262, the same clone but in a different formulation.

      Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Positive staining on rat skin.

      The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

      This data was produced using ab308262, the same clone but in a different formulation.

      Immunohistochemical analysis of paraffin-embedded cell pellets labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Cytoplasmic staining on (A) HeLa cell pellet. No staining on (B) Raji cell pellet.

      The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

      This data was produced using ab308262, the same clone but in a different formulation.

      Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Negative control: No staining on human skeletal muscle.

      The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

      This data was produced using ab308262, the same clone but in a different formulation.

      Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Negative control: No staining on mouse skeletal muscle.

      The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

      This data was produced using ab308262, the same clone but in a different formulation.

      Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Negative control: No staining on rat skeletal muscle.

      The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Dot Blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Dot Blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

      This data was produced using ab308262, the same clone but in a different formulation.

      Dot blot analysis of pan cytokeratin using ab308262 at 1:1000 dilution (0.541 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

      Exposure time: 180 seconds.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Lane 1: Peptides for KRT5, KRT6, KRT7, KRT8
      Lane 2: Peptide for KRT2
      Lane 3: Peptide for KRT1
      Lane 4: Peptide for KRT3
      Lane 5: Peptide for KRT4
      Lane 6: Peptide for KRT23
      Lane 7: Peptides for KRT10, KRT12, KRT15, KRT18, KRT25, KRT26, KRT28
      Lane 8: Peptide for KRT20
      Lane 9: Peptide for KRT9
      Lane 10: Peptides for KRT14, KRT16, KRT17, KRT19, KRT27
      Lane 11: Peptide for KRT24

    • Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)
      Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (ab308263)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab308263? Please let us know so that we can cite the reference in this datasheet.

    ab308263 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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