Anti-pan Cytokeratin antibody [KRT/1877R] (ab234297)
Key features and details
- Rabbit monoclonal [KRT/1877R] to pan Cytokeratin
- Suitable for: ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-pan Cytokeratin antibody [KRT/1877R]
See all pan Cytokeratin primary antibodies -
Description
Rabbit monoclonal [KRT/1877R] to pan Cytokeratin -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Goat, Guinea pig, Cow, Pig, Monkey -
Immunogen
Tissue, cells or virus corresponding to Human pan Cytokeratin. (Keratin-enriched preparation from cultured human epithelial cells).
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Positive control
- IHC-P: Human skin tissue. ICC: MCF7 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
KRT/1877R -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 1 - 2 µg/ml.
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IHC-P | (4) |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at room temperature. |
Notes |
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ICC
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation for 30 minutes at room temperature. |
Target
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Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
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Alternative names
- pan ck antibody
- pan-ck antibody
- panck antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [KRT/1877R] (ab234297)
Formalin-fixed, paraffin-embedded human skin tissue stained for pan Cytokeratin using ab234297 at 1 μg/ml in immunohistochemical analysis.
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Immunocytochemistry analysis of MCF7 cells labeling pan Cytokeratin with ab234297 at 2ug/mL followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab234297 has been referenced in 4 publications.
- Moon H et al. Activated TAZ induces liver cancer in collaboration with EGFR/HER2 signaling pathways. BMC Cancer 22:423 (2022). PubMed: 35439973
- Huang N et al. SARS-CoV-2 infection of the oral cavity and saliva. Nat Med 27:892-903 (2021). PubMed: 33767405
- Xu L et al. MEF/KSF-conditioned culture medium: An effective method for in vitro culture of mouse dermal papilla cells with osteogenic differentiation potential. Exp Ther Med 22:828 (2021). PubMed: 34149874
- Huang N et al. Integrated Single-Cell Atlases Reveal an Oral SARS-CoV-2 Infection and Transmission Axis. medRxiv N/A:N/A (2020). PubMed: 33140061