Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] - BSA and Azide free (ab216014)
Key features and details
- Mouse monoclonal [PAN-CK (Cocktail)] to pan Cytokeratin - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] - BSA and Azide free
See all pan Cytokeratin primary antibodies -
Description
Mouse monoclonal [PAN-CK (Cocktail)] to pan Cytokeratin - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Monkey -
Immunogen
Full length native protein (purified) corresponding to Human pan Cytokeratin. Human epidermal keratins
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Positive control
- Human skin tissue.
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General notes
Isotype is mouse IgGs, kappa.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab216014 is purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
PAN-CK (Cocktail) -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(for 30 minutes at RT) |
Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (for 30 minutes at RT) |
Target
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Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
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Alternative names
- pan ck antibody
- pan-ck antibody
- panck antibody
Images
Datasheets and documents
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Datasheet download
References (0)
ab216014 has not yet been referenced specifically in any publications.