Anti-pan PDK antibody [3H5BE12] (ab115321)
![Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)](https://www.abcam.com/ps/products/115/ab115321/Images/ab115321-127377-anti-pan-pdk-antibody-3h5be12-western-blot.jpg "Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)")
Key features and details
- Mouse monoclonal [3H5BE12] to pan PDK
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-pan PDK antibody [3H5BE12] -
Description
Mouse monoclonal [3H5BE12] to pan PDK -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
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General notes
PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Near homogeneity as judged by SDS-PAGE (>95% purity). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Primary antibody notes
PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH. -
Clonality
Monoclonal -
Clone number
3H5BE12 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.
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| Notes |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. |
Images
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Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)All lanes : Anti-pan PDK antibody [3H5BE12] (ab115321) at 1 µg/ml
Lane 1 : human heart mitochondria at 20 µg
Lane 2 : human liver mitochondria at 10 µg
Lane 3 : rat liver mitochondria at 10 µg
Lane 4 : mouse liver mitochondria at 10 µg
Lane 5 : PDK1 recombinant protein ab110359
at 0.1 µg
Lane 6 : PDK2 recombinant protein ab110354 at 0.1 µg
Lane 7 : PDK3 recombinant protein ab110355 at 0.1 µg
Lane 8 : PDK4 recombinant protein ab110356 at 0.1 µg
Lane 9 : PDK1 recombinant protein ab86986 at 0.1 µg
Secondary
All lanes : GAM IR 800 1:5000
Performed under reducing conditions.
Predicted band size: 49 kDa
ab115321 identifies all four isoforms of PDKs (PDK1, PDK2, PDK3, and PDK4) with differential affinity. PDK3 has the highest affinity, then PDK2 and 4, it has the lowest affinity to PDK1. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan PDK antibody [3H5BE12] (ab115321)](https://www.abcam.com/ps/products/115/ab115321/Images/ab115321-127376-anti-pan-pdk-antibody-3h5be12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan PDK antibody [3H5BE12] (ab115321)IHC image of ab115321 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab115321, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)](https://www.abcam.com/ps/products/115/ab115321/Images/ab115321-229070-anti-pan-pdk-antibody-3h5be12-western-blot.jpg)
Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)This image is courtesy of an anonymous AbreviewWestern blot analysis of human ovarian cancer cell lines whole cell lysate (30µg/lane) labelling pan PDK with ab115321 at 1/1000. A HRP-conjugated goat anti-mouse IgG polyclonal (1/10000) was used as the secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab115321 has been referenced in 2 publications.
- Xiao ZD et al. Energy stress-induced lncRNA FILNC1 represses c-Myc-mediated energy metabolism and inhibits renal tumor development. Nat Commun 8:783 (2017). PubMed: 28978906
- Koukourakis MI et al. Metabolic cooperation between co-cultured lung cancer cells and lung fibroblasts. Lab Invest 97:1321-1331 (2017). PubMed: 28846077
