Recombinant Anti-Pan Trk antibody [EPR18413] (ab189903)
![Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)](https://www.abcam.com/ps/products/189/ab189903/Images/ab189903-260561-anti-pan-trk-antibody-epr18413-western-blot.jpg "Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18413] to Pan Trk
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Pan Trk antibody [EPR18413]
See all Pan Trk primary antibodies -
Description
Rabbit monoclonal [EPR18413] to Pan Trk -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human, mouse and rat brain lysates; SH-SY5Y whole cell lysate; HEK-293 whole cell lysate transfected with TrkA, TrkB and TrkC, respectively. IP: Mouse brain whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18413 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/40.
|
|
| WB |
1/1000. Detects a band of approximately 140,30 kDa (predicted molecular weight: 94 kDa).
|
| Notes |
|---|
|
IP
1/40. |
|
WB
1/1000. Detects a band of approximately 140,30 kDa (predicted molecular weight: 94 kDa). |
Target
-
Relevance
Family of neurotrophic tyrosine kinase (NTRK1/2/3) genes which encode TrkA, TrkB and TrkC protein kinases. The three family members are activated by different neurotrophins: TrkA is activated by Nerve growth factor (NGF), TrkB by Brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4) and TrkC by NT-3. Neurotrophin signalling activates cellular pathways involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. Localization TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NT-3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane. -
Database links
- Entrez Gene: 4914 Human
- Entrez Gene: 4915 Human
- Entrez Gene: 4916 Human
- Entrez Gene: 18211 Mouse
- Entrez Gene: 18212 Mouse
- Entrez Gene: 18213 Mouse
- Entrez Gene: 25054 Rat
- Entrez Gene: 29613 Rat
see all -
Alternative names
- BDNF/NT-3 growth factors receptor antibody
- EML4 antibody
- ETV6 antibody
see all
Images
-
Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 94 kDa
Observed band size: 140,30 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.
-
![Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)](https://www.abcam.com/ps/products/189/ab189903/Images/ab189903-260560-anti-pan-trk-antibody-epr18413-western-blot.jpg)
Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 94 kDa
Observed band size: 140,30 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.
-
![Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)](https://www.abcam.com/ps/products/189/ab189903/Images/ab189903-260559-anti-pan-trk-antibody-epr18413-western-blot.jpg)
Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/5000 dilution
Lanes 1 & 3 & 5 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkA with His/Myc-tag
Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB with His/Myc-tag
Lane 6 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkC with His/Myc-tag
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 94 kDaExposure times: Lane 1-2: 1 minute; Lane 3-6: 30 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunoprecipitation - Anti-Pan Trk antibody [EPR18413] (ab189903)](https://www.abcam.com/ps/products/189/ab189903/Images/ab189903-260558-anti-pan-trk-antibody-epr18413-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Pan Trk antibody [EPR18413] (ab189903)TrkC (phospho Y820) was immunoprecipitated from 1 mg of mouse brain whole cell lysate with ab189903 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab189903 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: Mouse brain whole cell lysate 10 µg (Input).
Lane 2: ab189903 IP in mouse brain whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189903 in mouse brain whole cell lysate.
Exposure time: 5 seconds.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
-
![Anti-Pan Trk antibody [EPR18413] (ab189903)](https://www.abcam.com/ps/products/189/ab189903/Images/ab189903-6-benefits-of-recombinant-antibodies.png)
Anti-Pan Trk antibody [EPR18413] (ab189903)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab189903 has not yet been referenced specifically in any publications.