Name: Anti-PAP antibody [EPR4066]
Brand: RabMAb
SKU: ab108984
Rating: 4 (1 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4066] to PAP
Suitable for: WB, IP, IHC-P
Reacts with: Human

Carrier Free

Product name

Anti-PAP antibody [EPR4066]
See all PAP primary antibodies
Description

Rabbit monoclonal [EPR4066] to PAP
Host species

Rabbit
Tested applications

Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Prostate cancer cell lysate. IHC: human prostatic and human prostatic adenocarcinoma tissues. IP: human prostate lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.41 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4066
Isotype

IgG
Research areas

Alternative Versions
- Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human PAP protein (Active) (ab219224)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP		1/10 - 1/100.
IHC-P	(1)	1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended.

Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended.

Application notes

Is unsuitable for Flow Cyt.

Sequence similarities

Belongs to the histidine acid phosphatase family.
Cellular localization

Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
Target information above from: UniProt accession P15309 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 55 Human
- Omim: 171790 Human
- SwissProt: P15309 Human
- Unigene: 433060 Human
Alternative names
- 5' NT antibody
- 5' nucleotidase antibody
- 5'-NT antibody
- 5'-nucleotidase antibody
- Acid phosphatase prostate antibody
- ACP 3 antibody
- ACP3 antibody
- acpP antibody
- Ecto 5' nucleotidase antibody
- Ecto-5'-nucleotidase antibody
- PAP antibody
- PPAP_HUMAN antibody
- Prostatic acid phosphatase antibody
- Prostatic acid phosphotase antibody
- Thiamine monophosphatase antibody
- TMPase antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

Immunohistochemical staining of paraffin-embedded human prostatic tissue using anti-PAP (ab108984).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-PAP antibody [EPR4066] (ab108984)

Anti-PAP antibody [EPR4066] (ab108984) at 1/1000 dilution + Prostate cancer cell lysate at 10 µg

Predicted band size: 45 kDa
Immunoprecipitation - Anti-PAP antibody [EPR4066] (ab108984)

PAP was immunoprecipitated from 0.35 mg Human prostate whole cell lysate 10 µg with 108984 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Human prostate whole cell lysate 10 µg

Lane 2: ab108984 IP in Human prostate whole cell lysate

Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab108984 in Human prostate whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

Immunohistochemical staining of paraffin-embedded human prostatic adenocarcinoma tissue using anti-PAP (ab108984).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Normal placenta tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing positive staining in Prostatic carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Cervical carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Normal breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-PAP antibody [EPR4066] (ab108984)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-PAP antibody [EPR4066] (ab108984)

Immunoprecipitation protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab108984? Please let us know so that we can cite the reference in this datasheet.

ab108984 has been referenced in 3 publications.

Liu T et al. FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness. Proc Natl Acad Sci U S A 114:7683-7688 (2017). PubMed: 28674022
Wei J et al. Rapid progression of mixed neuroendocrine carcinoma-acinar adenocarcinoma of the prostate: A case report. Oncol Lett 12:1019-1022 (2016). IHC-P ; Human . PubMed: 27446387
Ge R et al. DNA methyl transferase 1 reduces expression of SRD5A2 in the aging adult prostate. Am J Pathol 185:870-82 (2015). PubMed: 25700986

Customer reviews and Q&As

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Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Prostate Cancer)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA based buffer pH 9.0

Permeabilization

Specification

Prostate Cancer

Blocking step

Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 23°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 13 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (3)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Prostatic Acid Phosphatase antibody [EPR4066]

Dissociation constant (K_D)