Anti-PARK7/DJ1 antibody (ab18257)
Key features and details
- Rabbit polyclonal to PARK7/DJ1
- Suitable for: IHC-P, ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PARK7/DJ1 antibody
See all PARK7/DJ1 primary antibodies -
Description
Rabbit polyclonal to PARK7/DJ1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Dog
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Immunogen
Synthetic peptide corresponding to Human PARK7/DJ1 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab18659) -
Positive control
- WB: HeLa, Jurkat, PC12 and NIH 3T3 whole cell lysates, mouse brain, liver, heart, kidney, pancreas, testis, skeletal muscle, spinal cord and ovary tissue lysates and rat brain, liver, heart and kidney tissue lysates. IHC-P: Human parathyroid tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF | (5) |
Use a concentration of 1 µg/ml.
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| WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 20 kDa).
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 20 kDa). |
Target
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Function
Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone. -
Tissue specificity
Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain. Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. -
Involvement in disease
Defects in PARK7 are the cause of Parkinson disease type 7 (PARK7) [MIM:606324]. A neurodegenerative disorder characterized by resting tremor, postural tremor, bradykinesia, muscular rigidity, anxiety and psychotic episodes. PARK7 has onset before 40 years, slow progression and initial good response to levodopa. Some patients may show traits reminiscent of amyotrophic lateral sclerosis-parkinsonism/dementia complex (Guam disease). -
Sequence similarities
Belongs to the peptidase C56 family. -
Post-translational
modificationsSumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.
Cys-106 is easily oxidized to sulfinic acid.
Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. -
Cellular localization
Cytoplasm. Nucleus. Mitochondrion. Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients. - Information by UniProt
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Database links
- Entrez Gene: 511268 Cow
- Entrez Gene: 11315 Human
- Entrez Gene: 57320 Mouse
- Entrez Gene: 117287 Rat
- Omim: 602533 Human
- SwissProt: Q5E946 Cow
- SwissProt: Q99497 Human
- SwissProt: Q99LX0 Mouse
see all -
Alternative names
- CAP1 antibody
- DJ-1 antibody
- DJ1 antibody
see all
Images
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Western blot - Anti-PARK7/DJ1 antibody (ab18257)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PARK7/DJ1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab18257 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab18257 was shown to specifically react with PARK/DJ1 in wild-type HAP1 cells. No band was observed when PARK/DJ1 knockout samples were used. Wild-type and PARK/DJ1 knockout samples were subjected to SDS-PAGE. ab18257 and ab8245 (loading control to GAPDH) were diluted to 1µg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging. -
Western blot - Anti-PARK7/DJ1 antibody (ab18257)All lanes : Anti-PARK7/DJ1 antibody (ab18257) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Kidney (Mouse) Tissue Lysate
Lane 5 :Mouse pancreas tissue lysate - total protein (ab29363)
Lane 6 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 7 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 8 : Spinal Cord (Mouse) Tissue Lysate
Lane 9 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 10 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 11 : Brain (Rat) Tissue Lysate - normal tissue
Lane 12 : Liver (Rat) Tissue Lysate
Lane 13 : Heart (Rat) Tissue Lysate
Lane 14 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody (ab18257)ab18257 staining PARK7/DJ1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab18257 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-PARK7/DJ1 antibody (ab18257)All lanes : Anti-PARK7/DJ1 antibody (ab18257) at 1 µg/ml
Lane 1 : Jurkat lysate
Lane 2 : HeLa lysate
Lane 3 : 3T3 lysate
Lane 4 : Jurkat lysate withHuman PARK7/DJ1 peptide (ab18659) at 1 µg/ml
Lane 5 : HeLa lysate withHuman PARK7/DJ1 peptide (ab18659) at 1 µg/ml
Lane 6 : 3T3 lysate withHuman PARK7/DJ1 peptide (ab18659) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (680) at 1/10000 dilution
Predicted band size: 20 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted? -
Western blot - Anti-PARK7/DJ1 antibody (ab18257)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab18257 has been referenced in 45 publications.
- Tokarew JM et al. Age-associated insolubility of parkin in human midbrain is linked to redox balance and sequestration of reactive dopamine metabolites. Acta Neuropathol 141:725-754 (2021). PubMed: 33694021
- Todkar K et al. Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs. Nat Commun 12:1971 (2021). PubMed: 33785738
- Elakad O et al. Role of Annexin A1 in Squamous Cell Lung Cancer Progression. Dis Markers 2021:5520832 (2021). PubMed: 33959206
- Sánchez-Lanzas R & Castaño JG Mitochondrial LonP1 protease is implicated in the degradation of unstable Parkinson's disease-associated DJ-1/PARK 7 missense mutants. Sci Rep 11:7320 (2021). PubMed: 33795807
- Yin L et al. PARK7 Protects Against Chronic Kidney Injury and Renal Fibrosis by Inducing SOD2 to Reduce Oxidative Stress. Front Immunol 12:690697 (2021). PubMed: 34093596