Recombinant Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)
![Western blot - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)](https://www.abcam.com/ps/products/201/ab201147/Images/ab201147-285393-anti-park7-dj1-antibody-epr19466-105-western-blot.jpg "Western blot - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19466-105] to PARK7/DJ1
- Suitable for: WB, ICC/IF, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PARK7/DJ1 antibody [EPR19466-105]
See all PARK7/DJ1 primary antibodies -
Description
Rabbit monoclonal [EPR19466-105] to PARK7/DJ1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB; HeLa, HEK-293T and LNCaP whole cell lysate. Human brain, fetal heart, fetal kidney and fetal spleen lysate. ICC/IF: HeLa and LNCaP cells. Flow Cyt (intra): HeLa cells. IP: HEK-293T whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19466-105 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 20 kDa).
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| ICC/IF |
1/100.
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| IP |
1/60.
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| Flow Cyt (Intra) |
1/120.
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| Notes |
|---|
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WB
1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 20 kDa). |
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ICC/IF
1/100. |
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IP
1/60. |
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Flow Cyt (Intra)
1/120. |
Target
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Function
Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone. -
Tissue specificity
Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain. Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. -
Involvement in disease
Defects in PARK7 are the cause of Parkinson disease type 7 (PARK7) [MIM:606324]. A neurodegenerative disorder characterized by resting tremor, postural tremor, bradykinesia, muscular rigidity, anxiety and psychotic episodes. PARK7 has onset before 40 years, slow progression and initial good response to levodopa. Some patients may show traits reminiscent of amyotrophic lateral sclerosis-parkinsonism/dementia complex (Guam disease). -
Sequence similarities
Belongs to the peptidase C56 family. -
Post-translational
modificationsSumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.
Cys-106 is easily oxidized to sulfinic acid.
Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. -
Cellular localization
Cytoplasm. Nucleus. Mitochondrion. Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients. - Information by UniProt
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Database links
- Entrez Gene: 11315 Human
- Omim: 602533 Human
- SwissProt: Q99497 Human
- Unigene: 419640 Human
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Alternative names
- CAP1 antibody
- DJ-1 antibody
- DJ1 antibody
see all
Images
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Western blot - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)All lanes : Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147) at 1/5000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human embryonic kidney) whole cell lysate
Lane 3 : LNCaP (human prostate carcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 20 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)](https://www.abcam.com/ps/products/201/ab201147/Images/ab201147-285388-anti-park7-dj1-antibody-epr19466-105-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)Immunofluorescent analysis of 100% methanol fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PARK7/DJ1 with ab201147 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining on HeLa cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)(ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)](https://www.abcam.com/ps/products/201/ab201147/Images/ab201147-285373-anti-park7-dj1-antibody-epr19466-105-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed HeLa (human cervix adenocarcinoma) cell line labeling PARK7/DJ1 with ab201147 at 1/120 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)](https://www.abcam.com/ps/products/201/ab201147/Images/ab201147-285371-anti-park7-dj1-antibody-epr19466-105-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)PARK7/DJ1 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) whole cell lysate with ab201147 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab201147 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293T (human embryonic kidney) whole cell lysate 10 μg (Input).
Lane 2: HEK-293T whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201147 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds. -
![Western blot - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)](https://www.abcam.com/ps/products/201/ab201147/Images/ab201147-285390-anti-park7-dj1-antibody-epr19466-105-western-blot.jpg)
Western blot - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)All lanes : Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147) at 1/5000 dilution
Lane 1 : Human brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 20 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and dilution buffer: 5% NFDM/TBST.
-
![Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)](https://www.abcam.com/ps/products/201/ab201147/Images/ab201147-285376-anti-park7-dj1-antibody-epr19466-105-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)Immunofluorescent analysis of 100% methanol fixed LNCaP (Human prostate cancer cell line) cells labeling PARK7/DJ1 with ab201147 at 1/100 dilution, followed by AlexaFluor®488 Goat Anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining on LNCaP cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)(ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)](https://www.abcam.com/ps/products/201/ab201147/Images/ab201147-8-benefits-of-recombinant-antibodies.png)
Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab201147 has been referenced in 1 publication.
- Xue Y & Wang AZ DJ-1 plays a neuroprotective role in SH-SY5Y cells by modulating Nrf2 signaling in response to lidocaine-mediated oxidative stress and apoptosis. Kaohsiung J Med Sci 36:630-639 (2020). WB ; Human . PubMed: 32363780