Recombinant Anti-PARN antibody [EPR11670(2)] (ab188333)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11670(2)] to PARN
- Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PARN antibody [EPR11670(2)]
See all PARN primary antibodies -
Description
Rabbit monoclonal [EPR11670(2)] to PARN -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562, Hela, MDA-MB435, PC-12, NIH-3T3 cell lysates; IHC-P: Human papillary adenocarcinoma of thyroid, Mouse liver; ICC/IF: Hela cells; Flow Cyt (intra): Hela cells; IP: K562 celll lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11670(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/70.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
1/50.
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ICC/IF |
1/100.
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WB |
1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 73 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/50. |
ICC/IF
1/100. |
WB
1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 73 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the CAF1 family.
Contains 1 R3H domain. -
Cellular localization
Nucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar. - Information by UniProt
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Database links
- Entrez Gene: 5073 Human
- Entrez Gene: 74108 Mouse
- Entrez Gene: 360464 Rat
- Omim: 604212 Human
- SwissProt: O95453 Human
- SwissProt: Q8VDG3 Mouse
- Unigene: 253197 Human
- Unigene: 182350 Mouse
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Alternative names
- DAN antibody
- Deadenylating nuclease antibody
- Deadenylation nuclease antibody
see all
Images
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All lanes : Anti-PARN antibody [EPR11670(2)] (ab188333) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MDA-MB-435 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 73 kDa -
Immunofluorescent analysis of HeLa cells labelling PARN with ab188333 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary antibody. Nuclei counterstained with DAPI (blue).
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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All lanes : Anti-PARN antibody [EPR11670(2)] (ab188333) at 1/1000 dilution
Lane 1 : PC-12 cell lysate
Lane 2 : NIH-3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 73 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARN antibody [EPR11670(2)] (ab188333)
Immunohistochemical analysis of formalin fixed paraffin embedded Human papillary adenocarcinoma of thyroid labeling PARN with ab188333 at 1/100 dilution and HRP polymer for Rabbit IgG. Counterstained with Haematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARN antibody [EPR11670(2)] (ab188333)
Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labeling PARN with ab188333 at 1/100 dilution and HRP polymer for Rabbit IgG. Counterstained with Haematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of Hela cells fixed in 4% paraformaldehyde labeling PARN with ab188333 at 1/100 and Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution. Counterstained with DAPI (blue).
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Intracellular flow cytometric analysis of HeLa cells fixed in 2% paraformaldehyde labeling PARN with ab188333 at 1/70 dilution andGoat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.
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Immunoprecipitation of K562 cell lysate labeling PARN with ab188333 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (7)
ab188333 has been referenced in 7 publications.
- Araya LE et al. Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling. ACS Chem Biol 16:2280-2296 (2021). PubMed: 34553588
- Duan TL et al. Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN). Cells 9:N/A (2020). PubMed: 31936572
- Benyelles M et al. Impaired telomere integrity and rRNA biogenesis in PARN-deficient patients and knock-out models. EMBO Mol Med 11:e10201 (2019). PubMed: 31273937
- Kroustallaki P et al. SMUG1 Promotes Telomere Maintenance through Telomerase RNA Processing. Cell Rep 28:1690-1702.e10 (2019). PubMed: 31412240
- Son A et al. PARN and TOE1 Constitute a 3' End Maturation Module for Nuclear Non-coding RNAs. Cell Rep 23:888-898 (2018). ICC/IF ; Human . PubMed: 29669292
- Takata MA et al. CG dinucleotide suppression enables antiviral defence targeting non-self RNA. Nature 550:124-127 (2017). PubMed: 28953888
- Moon DH et al. Poly(A)-specific ribonuclease (PARN) mediates 3'-end maturation of the telomerase RNA component. Nat Genet 47:1482-8 (2015). PubMed: 26482878