Recombinant Anti-Paxillin antibody [Y113] (ab32084)
![Western blot - Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.com/ps/products/32/ab32084/Images/ab32084-467544-anti-paxillin-antibody-y113-westernblot-human-mouse.jpg "Western blot - Anti-Paxillin antibody [Y113] (ab32084)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y113] to Paxillin
- Suitable for: WB, ICC/IF, Flow Cyt (Intra), IP, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Paxillin antibody [Y113]
See all Paxillin primary antibodies -
Description
Rabbit monoclonal [Y113] to Paxillin -
Host species
Rabbit -
Specificity
Anti-Paxillin antibody [Y113] (ab32084) recognises Paxillin alpha, beta and gamma isoforms.
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Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra), IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Dog
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): HeLa cells. ICC/IF: HeLa cells IP: HeLa whole cell lysate WB: HeLa, RAW 264.7 and mouse heart lysate. IHC-P: Human cerebrum tissue and human breast carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 4.17 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y113 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
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Substrate reagent
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (10) |
1/1000. Predicted molecular weight: 68 kDa.
For unpurified use at 1/5000 - 1/10000. |
| ICC/IF | (7) |
1/50.
For unpurified use at 1/250. |
| Flow Cyt (Intra) |
1/100.
|
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| IP | (1) |
1/20.
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| IHC-P | (1) |
1/1200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 68 kDa. For unpurified use at 1/5000 - 1/10000. |
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ICC/IF
1/50. For unpurified use at 1/250. |
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Flow Cyt (Intra)
1/100. |
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IP
1/20. |
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IHC-P
1/1200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). -
Sequence similarities
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. -
Cellular localization
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. - Information by UniProt
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Database links
- Entrez Gene: 5829 Human
- Entrez Gene: 19303 Mouse
- Omim: 602505 Human
- SwissProt: P49023 Human
- SwissProt: Q8VI36 Mouse
- Unigene: 446336 Human
- Unigene: 18714 Mouse
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Alternative names
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
see all
Images
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Western blot - Anti-Paxillin antibody [Y113] (ab32084)All lanes : Anti-Paxillin antibody [Y113] (ab32084) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : Mouse heart lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 68 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.com/ps/products/32/ab32084/Images/ab32084-499762-anti-paxillin-antibody-y113-immunohistochemisty-human-breast-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Paxillin antibody [Y113] (ab32084)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue sections labelling Paxillin with ab32084 at 1/1200 dilution. The section was incubated with ab32084 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Positive staining on human breast carcinoma tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
![Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.com/ps/products/32/ab32084/Images/ab32084-18-anti-paxillin-antibody-y113-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113] (ab32084)Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Paxillin with purified ab32084 at 1/50 dilution (2.88 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Flow Cytometry (Intracellular) - Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.com/ps/products/32/ab32084/Images/ab32084-276941-anti-paxillin-antibody-y113-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Paxillin antibody [Y113] (ab32084)Intracellular Flow Cytometry analysis ofHeLa (human cervix adenocarcinoma) cells labeling with purified ab32084 at 1/100 dilution (10µg/ml) (Red). Cells were fixed with4% paraformaldehydeand permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody.Rabbit monoclonal IgG (Black) (ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.com/ps/products/32/ab32084/Images/ab32084-499760-anti-paxillin-antibody-y113-immunohistochemisty-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Paxillin antibody [Y113] (ab32084)Immunohistochemistry analysis of paraffin-embedded human cerebrum tissue sections labelling Paxillin with ab32084 at 1/1200 dilution. The section was incubated with ab32084 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Positive staining on human cerebrum tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
![Immunoprecipitation - Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.com/ps/products/32/ab32084/Images/ab32084-19-anti-paxillin-antibody-y113-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-Paxillin antibody [Y113] (ab32084)Purified ab32084 at 1/20 dilution (0.7µg) immunoprecipitating Paxillin in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg.
Lane 2 (+): ab32084 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32084 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 48 kDa -
![OI-RD Scanning - Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.com/ps/products/32/ab32084/Images/ab32084-43827-anti-paxillin-antibody-y113.jpg)
OI-RD Scanning - Anti-Paxillin antibody [Y113] (ab32084) -
![Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.com/ps/products/32/ab32084/Images/ab32084-17-benefits-of-recombinant-antibodies.png)
Anti-Paxillin antibody [Y113] (ab32084)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (152)
ab32084 has been referenced in 152 publications.
- Kilinc AN et al. Integrin-linked kinase tunes cell-cell and cell-matrix adhesions to regulate the switch between apoptosis and EMT downstream of TGFß1. Mol Biol Cell 32:402-412 (2021). PubMed: 33405954
- Koual M et al. Aggressiveness and Metastatic Potential of Breast Cancer Cells Co-Cultured with Preadipocytes and Exposed to an Environmental Pollutant Dioxin: An in Vitro and in Vivo Zebrafish Study. Environ Health Perspect 129:37002 (2021). PubMed: 33683140
- Yeoman B et al. Adhesion strength and contractility enable metastatic cells to become adurotactic. Cell Rep 34:108816 (2021). PubMed: 33691109
- Wang L et al. Combination CTLA-4 immunoglobulin treatment and ultrasound microbubble-mediated exposure improve renal function in a rat model of diabetic nephropathy. Aging (Albany NY) 13:8524-8540 (2021). PubMed: 33714204
- Bain JM et al. Immune cells fold and damage fungal hyphae. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33876755