Recombinant Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA and Azide free (ab272073)
![Immunocytochemistry - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA and Azide free (ab272073)](https://www.abcam.com/ps/products/272/ab272073/Images/ab272073-1-ab243876334294antibaf180antibodybl392c3immunocytochemistry.jpg "Immunocytochemistry - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA and Azide free (ab272073)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-39-2C3] to PBRM1/BAF180 - BSA and Azide free
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA and Azide free
See all PBRM1/BAF180 primary antibodies -
Description
Rabbit monoclonal [BL-39-2C3] to PBRM1/BAF180 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PBRM1/BAF180 aa 1-300.
Database link: Q86U86 -
General notes
ab272073 is the carrier-free version of ab243876.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline -
Carrier free
Yes -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BL-39-2C3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| ICC |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at an assay dependent concentration. |
Target
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Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Act as a negative regulator of cell proliferation. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in PBRM1 are a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. -
Sequence similarities
Contains 2 BAH domains.
Contains 6 bromo domains.
Contains 1 HMG box DNA-binding domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55193 Human
- Omim: 606083 Human
- SwissProt: Q86U86 Human
- Unigene: 189920 Human
- Unigene: 476320 Human
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Alternative names
- BAF180 antibody
- BRG1-associated factor 180 antibody
- CG11375 antibody
see all
Images
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Immunocytochemistry - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA and Azide free (ab272073)
Formaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling PBRM1/BAF180 using ab243876 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA and Azide free (ab272073)](https://www.abcam.com/ps/products/272/ab272073/Images/ab272073-2-ab243876334293antibaf180antibodybl392c3immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA and Azide free (ab272073)Formalin-fixed, paraffin-embedded human Breast tissue stained for PBRM1/BAF180 using ab243876 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).
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![Immunoprecipitation - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA and Azide free (ab272073)](https://www.abcam.com/ps/products/272/ab272073/Images/ab272073-3-ab243876334292antibaf180antibodybl392c3immunoprecipitation.jpg)
Immunoprecipitation - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA and Azide free (ab272073)PBRM1/BAF180 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243876 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243876 at 1/1000 dilution.
Lane 1: ab243876 IP in HEK-293T whole cell lysate.
Lane 2: Contol IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).
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![Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA and Azide free (ab272073)](https://www.abcam.com/ps/products/272/ab272073/Images/ab272073-4-benefits-of-recombinant-antibodies.png)
Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA and Azide free (ab272073)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab272073 has not yet been referenced specifically in any publications.