Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-PBX1/PRL antibody [EPR15937] (ab192606)

Submit a review Submit a question References (1)
Immunoprecipitation - Anti-PBX1/PRL antibody [EPR15937] (ab192606)
  • Western blot - Anti-PBX1/PRL antibody [EPR15937] (ab192606)
  • Anti-PBX1/PRL antibody [EPR15937] (ab192606)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15937] to PBX1/PRL
  • Suitable for: IP, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-PBX1/PRL antibody [EPR15937]
    See all PBX1/PRL primary antibodies

  • Description

    Rabbit monoclonal [EPR15937] to PBX1/PRL

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Hela, Jurkat, A431 cell lysates

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab192606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/50.
WB
1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa).
Notes
IP
1/50.
WB
1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa).

Target

  • Function

    Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation.

  • Tissue specificity

    Expressed in all tissues except in cells of the B and T lineage.

  • Involvement in disease

    Note=A chromosomal aberration involving PBX1 is a cause of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with TCF3. E2A-PBX1 transforms cells by constitutively activating transcription of genes regulated by PBX1 or by other members of the PBX protein family.

  • Sequence similarities

    Belongs to the TALE/PBX homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • DKFZp686B09108 antibody
      • Homeo box protein PBX1 antibody
      • Homeo box protein PRL antibody
      • Homeobox protein PBX 1 antibody
      • Homeobox protein PBX1 antibody
      • Homeobox protein PRL antibody
      • MGC126627 antibody
      • PBX 1 antibody
      • Pbx1 antibody
      • PBX1_HUMAN antibody
      • Pre B cell leukemia homeobox 1 antibody
      • Pre B cell leukemia transcription factor 1 antibody
      • Pre-B-cell leukemia transcription factor 1 antibody
      • PRL antibody
      see all

    Images

    • Immunoprecipitation - Anti-PBX1/PRL antibody [EPR15937] (ab192606)
      Immunoprecipitation - Anti-PBX1/PRL antibody [EPR15937] (ab192606)

      Immunoprecipitation of Hela cell lysates (+) or PBS control (-) labeling PBX1 using ab192606 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 as secondary.

    • Western blot - Anti-PBX1/PRL antibody [EPR15937] (ab192606)
      Western blot - Anti-PBX1/PRL antibody [EPR15937] (ab192606)
      All lanes : Anti-PBX1/PRL antibody [EPR15937] (ab192606) at 1/50000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

      Predicted band size: 47 kDa
      Additional bands at: 38 kDa (possible isoform)

    • Anti-PBX1/PRL antibody [EPR15937] (ab192606)
      Anti-PBX1/PRL antibody [EPR15937] (ab192606)

    References (1)

    Publishing research using ab192606? Please let us know so that we can cite the reference in this datasheet.

    ab192606 has been referenced in 1 publication.

    • Lin YJ  et al. The cooperation of cis-elements during M-cadherin promoter activation. Biochem J 478:911-926 (2021). PubMed: 33527978

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab192606.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com