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Anti-PCB antibody [10G7BF7] (ab123451)

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Western blot - Anti-PCB antibody [10G7BF7] (ab123451)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [10G7BF7] (ab123451)

Key features and details

  • Mouse monoclonal [10G7BF7] to PCB
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PCB antibody [10G7BF7]
    See all PCB primary antibodies

  • Description

    Mouse monoclonal [10G7BF7] to PCB

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • HepG2, Rat H9C2, Rat H4IIE, Mouse MEF cell lysates; Purified Cow liver PCB; HDFn cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab123451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 130 kDa.
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 130 kDa.

Target

  • Function

    Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.

  • Pathway

    Carbohydrate biosynthesis; gluconeogenesis.

  • Involvement in disease

    Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.

  • Sequence similarities

    Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • mitochondrial antibody
    • OTTHUMP00000235155 antibody
    • OTTHUMP00000235156 antibody
    • PC antibody
    • PCB antibody
    • Pcx antibody
    • PYC_HUMAN antibody
    • Pyruvate carboxylase antibody
    • Pyruvate carboxylase mitochondrial antibody
    • Pyruvic carboxylase antibody
    see all

Images

  • Western blot - Anti-PCB antibody [10G7BF7] (ab123451)
    Western blot - Anti-PCB antibody [10G7BF7] (ab123451)
    All lanes : Anti-PCB antibody [10G7BF7] (ab123451) at 1 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : Hela
    Lane 3 : H9C2
    Lane 4 : MEF
    Lane 5 : Recombinant PC

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat-anti-Mouse HRP at 1/3000 dilution

    Predicted band size: 130 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [10G7BF7] (ab123451)
    Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [10G7BF7] (ab123451)
    ab123451 at 1µg/ml staining PCB in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).

References (0)

Publishing research using ab123451? Please let us know so that we can cite the reference in this datasheet.

ab123451 has not yet been referenced specifically in any publications.

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