Anti-PCB antibody [3H2AD9] (ab110314)
![Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [3H2AD9] (ab110314)](https://www.abcam.com/ps/products/110/ab110314/Images/ab110314-123596-anti-pcb-antibody-3h2ad9-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [3H2AD9] (ab110314)")
Key features and details
- Mouse monoclonal [3H2AD9] to PCB
- Suitable for: ICC/IF, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PCB antibody [3H2AD9]
See all PCB primary antibodies -
Description
Mouse monoclonal [3H2AD9] to PCB -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Human fibroblast and HeLa cells; Human liver tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
ab110314 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
3H2AD9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml. For 2 hours.
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. For 2 hours. |
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Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IP
Use at an assay dependent concentration. |
Target
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Function
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. -
Pathway
Carbohydrate biosynthesis; gluconeogenesis. -
Involvement in disease
Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia. -
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 5091 Human
- Omim: 608786 Human
- SwissProt: P11498 Human
- Unigene: 89890 Human
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Alternative names
- mitochondrial antibody
- OTTHUMP00000235155 antibody
- OTTHUMP00000235156 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [3H2AD9] (ab110314)Upper image shows: Immunocytochemistry analysis using ab110314 at 5µg/ml staining PCB in Human fibroblast cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: The target protein locates to the mitochondrial matrix.
Lower image shows cells co-stained with an antibody against PDH (green), an enzyme also located in the mitochondrial matrix. The composite image shows an identical mitochondrial pattern for both antibodies indicated by merged orange color. -
![Immunoprecipitation - Anti-PCB antibody [3H2AD9] (ab110314)](https://www.abcam.com/ps/products/110/ab110314/Images/ab110314-123595-anti-pcb-antibody-3h2ad9-immunoprecipitation.gif)
Immunoprecipitation - Anti-PCB antibody [3H2AD9] (ab110314)Detection of ab110314 by immunoprecipitation staining of 130kDa PCB in human liver lysate. -
![Flow Cytometry - Anti-PCB antibody [3H2AD9] (ab110314)](https://www.abcam.com/ps/products/110/ab110314/Images/ab110314-123594-anti-pcb-antibody-3h2ad9-flow-cytometry.gif)
Flow Cytometry - Anti-PCB antibody [3H2AD9] (ab110314)Flow cytometric analysis using ab110314 at 1µg/ml staining PCB in HeLa cells (blue). Isotype control antibody (red).
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab110314 has been referenced in 2 publications.
- Nagaraj R et al. Nuclear Localization of Mitochondrial TCA Cycle Enzymes as a Critical Step in Mammalian Zygotic Genome Activation. Cell 168:210-223.e11 (2017). PubMed: 28086092
- Lussey-Lepoutre C et al. Loss of succinate dehydrogenase activity results in dependency on pyruvate carboxylation for cellular anabolism. Nat Commun 6:8784 (2015). IF . PubMed: 26522426
