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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

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Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
  • Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
  • Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9760(B)] to PCBD1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-PCBD1 antibody [EPR9760(B)]
    See all PCBD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9760(B)] to PCBD1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human PCBD1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human ovary cancer, fetal heart, fetal liver, HEK293T and Caco-2 cell lysates. IHC-P: Human colon tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab138518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity.

  • Involvement in disease

    Defects in PCBD1 are the cause of BH4-deficient hyperphenylalaninemia type D (HPABH4D) [MIM:264070]; also known as hyperphenylalaninemia with primapterinuria. HPABH4D is characterized by the excretion of 7-substituted pterins in the urine of affected patients.

  • Sequence similarities

    Belongs to the pterin-4-alpha-carbinolamine dehydratase family.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic and/or nuclear.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 4 alpha hydroxy tetrahydropterin dehydratase antibody
    • 4-alpha-hydroxy-tetrahydropterin dehydratase antibody
    • 6 pyruvoyl tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) antibody
    • 6 pyruvoyl tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha antibody
    • DCoH antibody
    • Dimerization cofactor of hepatic nuclear factor 1 alpha antibody
    • Dimerization cofactor of hepatocyte nuclear factor 1 alpha antibody
    • Dimerization cofactor of hepatocyte nuclear factor 1-alpha antibody
    • Dimerization cofactor of HNF1 antibody
    • Dimerizing cofactor for HNF1 antibody
    • PCBD 1 antibody
    • PCBD antibody
    • PCBD1 antibody
    • PCD antibody
    • Phenylalanine hydroxylase stimulating protein antibody
    • Phenylalanine hydroxylase-stimulating protein antibody
    • PHS antibody
    • PHS_HUMAN antibody
    • Pterin 4 alpha carbinolamine dehydratase antibody
    • Pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) antibody
    • Pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha antibody
    • Pterin carbinolamine dehydratase antibody
    • Pterin-4-alpha-carbinolamine dehydratase antibody
    see all

Images

  • Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
    Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
    All lanes : Anti-PCBD1 antibody [EPR9760(B)] (ab138518) at 1/500 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : PCBD1 knockout HEK293T cell lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : Human ovary cancer tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 12 kDa
    Observed band size: 12 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab138518 observed at 12 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab138518 Anti-PCBD1 antibody [EPR9760(B)]  was shown to specifically react with PCBD1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266261 (knockout cell lysate ab258095) was used. Wild-type and PCBD1 knockout samples were subjected to SDS-PAGE. ab138518 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling PCBD1 with ab138518 antibody at a dilution of 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
    Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
    All lanes : Anti-PCBD1 antibody [EPR9760(B)] (ab138518) at 1/1000 dilution

    Lane 1 : Human ovary cancer cell lysate
    Lane 2 : Fetal heart cell lysate
    Lane 3 : Fetal liver cell lysate
    Lane 4 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

  • Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
    Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

References (0)

Publishing research using ab138518? Please let us know so that we can cite the reference in this datasheet.

ab138518 has not yet been referenced specifically in any publications.

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