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    products/primary-antibodies/pcbd1-antibody-epr9760b-ab138518.pdf

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Signal Transduction Metabolism Amino Acids
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

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Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
  • Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
  • Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9760(B)] to PCBD1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human PCBD1 protein (ab100942)
Primary
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Anti-DHFRL1 antibody (ab197258)
Knockout
Product image
Human PCBD1 knockout HEK-293T cell line (ab266261)

View more associated products

Overview

  • Product name

    Anti-PCBD1 antibody [EPR9760(B)]
    See all PCBD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9760(B)] to PCBD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PCBD1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human ovary cancer, fetal heart, fetal liver, HEK293T and Caco-2 cell lysates. IHC-P: Human colon tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9760(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors

Associated products

  • Alternative Versions

    • Anti-PCBD1 antibody [EPR9760(B)] - BSA and Azide free (ab248860)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PCBD1 knockout HEK-293T cell line (ab266261)
  • KO cell lysates

    • Human PCBD1 knockout HEK-293T cell lysate (ab258095)
  • Recombinant Protein

    • Recombinant Human PCBD1 protein (ab100942)
  • Related Products

    • Recombinant Human PCBD1 protein (ab100942)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab138518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity.
  • Involvement in disease

    Defects in PCBD1 are the cause of BH4-deficient hyperphenylalaninemia type D (HPABH4D) [MIM:264070]; also known as hyperphenylalaninemia with primapterinuria. HPABH4D is characterized by the excretion of 7-substituted pterins in the urine of affected patients.
  • Sequence similarities

    Belongs to the pterin-4-alpha-carbinolamine dehydratase family.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic and/or nuclear.
  • Target information above from: UniProt accession P61457 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5092 Human
    • Entrez Gene: 13180 Mouse
    • Entrez Gene: 29700 Rat
    • Omim: 126090 Human
    • SwissProt: P61457 Human
    • SwissProt: P61458 Mouse
    • SwissProt: P61459 Rat
    • Unigene: 3192 Human
    • Unigene: 39473 Mouse
    • Unigene: 81180 Rat
    see all
  • Alternative names

    • 4 alpha hydroxy tetrahydropterin dehydratase antibody
    • 4-alpha-hydroxy-tetrahydropterin dehydratase antibody
    • 6 pyruvoyl tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) antibody
    • 6 pyruvoyl tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha antibody
    • DCoH antibody
    • Dimerization cofactor of hepatic nuclear factor 1 alpha antibody
    • Dimerization cofactor of hepatocyte nuclear factor 1 alpha antibody
    • Dimerization cofactor of hepatocyte nuclear factor 1-alpha antibody
    • Dimerization cofactor of HNF1 antibody
    • Dimerizing cofactor for HNF1 antibody
    • PCBD 1 antibody
    • PCBD antibody
    • PCBD1 antibody
    • PCD antibody
    • Phenylalanine hydroxylase stimulating protein antibody
    • Phenylalanine hydroxylase-stimulating protein antibody
    • PHS antibody
    • PHS_HUMAN antibody
    • Pterin 4 alpha carbinolamine dehydratase antibody
    • Pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) antibody
    • Pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha antibody
    • Pterin carbinolamine dehydratase antibody
    • Pterin-4-alpha-carbinolamine dehydratase antibody
    see all

Images

  • Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
    Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
    All lanes : Anti-PCBD1 antibody [EPR9760(B)] (ab138518) at 1/500 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : PCBD1 knockout HEK293T cell lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : Human ovary cancer tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 12 kDa
    Observed band size: 12 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab138518 observed at 12 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab138518 Anti-PCBD1 antibody [EPR9760(B)]  was shown to specifically react with PCBD1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266261 (knockout cell lysate ab258095) was used. Wild-type and PCBD1 knockout samples were subjected to SDS-PAGE. ab138518 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling PCBD1 with ab138518 antibody at a dilution of 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
    Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
    All lanes : Anti-PCBD1 antibody [EPR9760(B)] (ab138518) at 1/1000 dilution

    Lane 1 : Human ovary cancer cell lysate
    Lane 2 : Fetal heart cell lysate
    Lane 3 : Fetal liver cell lysate
    Lane 4 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

  • Anti-PCBD1 antibody [EPR9760(B)] (ab138518)
    Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab138518? Please let us know so that we can cite the reference in this datasheet.

ab138518 has not yet been referenced specifically in any publications.

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