Recombinant Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510)
![Western blot - Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510)](https://www.abcam.com/ps/products/201/ab201510/Images/ab201510-488408-anti-pcgf3pcgf5-antibody-epr19475-western-blot-rat-and-mouse.jpg "Western blot - Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19475] to PCGF3+PCGF5
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PCGF3+PCGF5 antibody [EPR19475]
See all PCGF3+PCGF5 primary antibodies -
Description
Rabbit monoclonal [EPR19475] to PCGF3+PCGF5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wildtype mouse embryonic stem cell lysate, HepG2, Raji, 293T, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19475 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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| Notes |
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WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.
Target
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Relevance
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility -
Cellular localization
PCGF3: Nucleus. -
Database links
- Entrez Gene: 10336 Human
- Entrez Gene: 84333 Human
- Entrez Gene: 69587 Mouse
- Entrez Gene: 76073 Mouse
- Entrez Gene: 305624 Rat
- Entrez Gene: 681178 Rat
- SwissProt: Q3KNV8 Human
- SwissProt: Q86SE9 Human
see all
Images
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Western blot - Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510)All lanes : Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 28,30 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBSTThis blot was developed using a higher sensitivity ECL substrate.
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![Western blot - Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510)](https://www.abcam.com/ps/products/201/ab201510/Images/ab201510-488406-anti-pcgf3pcgf5-antibody-epr19475-western-blot-human.jpg)
Western blot - Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510)All lanes : Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510) at 1/1000 dilution
Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 28, 30 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST -
![Western blot - Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510)](https://www.abcam.com/ps/products/201/ab201510/Images/ab201510-267930-anti-pcgf3pcgf5-antibody-epr19475-western-blot.jpg)
Western blot - Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510)The data is kindly provided by our collaborator Dr. Jinzhong Qin (Nanjing University).All lanes : Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510) at 1/1000 dilution
Lane 1 : PCGF3 WT mouse embryonic stem cell lysate
Lane 2 : PCGF3 knockout mouse embryonic stem cell lysate
Lane 3 : PCGF3/PCGF5 knockout mouse embryonic stem cell lysate
Lysates/proteins at 100 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaBlocking buffer: 3% milk in PBST
Dilution buffer: 3% BSA in PBST.
Exposure time: 2-10 minutes.
The data is kindly provided by our collaborator Dr. Jinzhong Qin (Nanjing University).
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![Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510)](https://www.abcam.com/ps/products/201/ab201510/Images/ab201510-4-benefits-of-recombinant-antibodies.png)
Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab201510 has been referenced in 3 publications.
- Fursova NA et al. Synergy between Variant PRC1 Complexes Defines Polycomb-Mediated Gene Repression. Mol Cell 74:1020-1036.e8 (2019). PubMed: 31029541
- Anbunathan H et al. Integrative Copy Number Analysis of Uveal Melanoma Reveals Novel Candidate Genes Involved in Tumorigenesis Including a Tumor Suppressor Role for PHF10/BAF45a. Clin Cancer Res 25:5156-5166 (2019). PubMed: 31227497
- Zhao W et al. Essential Role for Polycomb Group Protein Pcgf6 in Embryonic Stem Cell Maintenance and a Noncanonical Polycomb Repressive Complex 1 (PRC1) Integrity. J Biol Chem 292:2773-2784 (2017). WB . PubMed: 28049731