Recombinant

Recombinant Anti-PCNA antibody [16D10] (ab252848)

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Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (ab252848)
  • Immunoprecipitation - Anti-PCNA antibody [16D10] (ab252848)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (ab252848)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (ab252848)
  • Anti-PCNA antibody [16D10] (ab252848)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [16D10] to PCNA
  • Suitable for: ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-PCNA antibody [16D10]
    See all PCNA primary antibodies

  • Description

    Rat monoclonal [16D10] to PCNA

  • Host species

    Rat

  • Tested applications

    Suitable for: ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: NIH/3T3, HeLa and C6 cells. IP: NIH/3T3 cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab252848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
IP
1/60.
Notes
ICC/IF
1/100.
IP
1/60.

Target

  • Function

    This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

  • Sequence similarities

    Belongs to the PCNA family.

  • Post-translational
    modifications

    Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.

  • Cellular localization

    Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ATLD2 antibody
    • cb16 antibody
    • Cyclin antibody
    • DNA polymerase delta auxiliary protein antibody
    • etID36690.10 antibody
    • fa28e03 antibody
    • fb36g03 antibody
    • HGCN8729 antibody
    • MGC8367 antibody
    • Mutagen-sensitive 209 protein antibody
    • OTTHUMP00000030189 antibody
    • OTTHUMP00000030190 antibody
    • PCNA antibody
    • Pcna/cyclin antibody
    • PCNA_HUMAN antibody
    • PCNAR antibody
    • Polymerase delta accessory protein antibody
    • Proliferating cell nuclear antigen antibody
    • wu:fa28e03 antibody
    • wu:fb36g03 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (ab252848)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (ab252848)

    Immunocytochemical analysis of 100% methanol-fixed NIH/3T3 (mouse embryonic fibroblast) cells labeling PCNA (green) using ab252848 at 1/100 dilution followed by ab150157 Goat Anti-Rat  IgG H&L  (Alexa Fluor® 488) at 1/1000 dilution (2µg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10µg/ml) followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (2µg/ml) (Red). Confocal image showing nuclear staining in NIH/3T3 cell line. The nuclear counterdstain was DAPI (Blue). 

    -ve control 1: ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).

    -ve control 2: ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).

  • Immunoprecipitation - Anti-PCNA antibody [16D10] (ab252848)
    Immunoprecipitation - Anti-PCNA antibody [16D10] (ab252848)

    PCNA was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate using ab252848 at 1/60 dilution (2µg in 0.35µg lysates). Western blot was performed on the immunoprecipitate using ab252848 at 1/1000 dilution (0.35µg/ml) followed by anti-Rat IgG H&L (HRP) (ab205720) secondary at 1/5000 dilution. 

    Lane 1: NIH/3T3 whole cell lysate 10 µg.

    Lane 2: ab252848 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rat monoclonal IgG instead of ab252848 in NIH/3T3 whole cell lysate.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (ab252848)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (ab252848)

    Immunocytochemical analysis of 100% methanol-fixed C6 (rat glial tumor glial cell) cells labeling PCNA (green) using ab252848 at 1/100 dilution followed by ab150157 Goat Anti-Rat  IgG H&L  (Alexa Fluor® 488) at 1/1000 dilution (2µg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10µg/ml) followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (2µg/ml) (Red). Confocal image showing nuclear staining in C6 cell line. The nuclear counterdstain was DAPI (Blue). 

    -ve control 1: ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).

    -ve control 2: ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (ab252848)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (ab252848)

    Immunocytochemical analysis of 100% methanol-fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PCNA (green) using ab252848 at 1/100 dilution followed by ab150157 Goat Anti-Rat  IgG H&L  (Alexa Fluor® 488) at 1/1000 dilution (2µg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10µg/ml) followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (2µg/ml) (Red). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterdstain was DAPI (Blue). 

    -ve control 1: ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).

    -ve control 2: ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).

  • Anti-PCNA antibody [16D10] (ab252848)
    Anti-PCNA antibody [16D10] (ab252848)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab252848? Please let us know so that we can cite the reference in this datasheet.

ab252848 has been referenced in 1 publication.

  • Spada F  et al. DNMT1 but not its interaction with the replication machinery is required for maintenance of DNA methylation in human cells. J Cell Biol 176:565-71 (2007). PubMed: 17312023

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