Recombinant Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)
![Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)](https://www.abcam.com/ps/products/255/ab255842/Images/ab255842-397075-anti-pcna-antibody-16d10-immunocytochemistry-nih3t3-cells.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [16D10] to PCNA - BSA and Azide free
- Suitable for: IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PCNA antibody [16D10] - BSA and Azide free
See all PCNA primary antibodies -
Description
Rat monoclonal [16D10] to PCNA - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: NIH/3T3, HeLa and C6 cells. IP: NIH/3T3 cell lysate.
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General notes
ab255842 is the carrier-free version of ab252848.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
16D10 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/60.
|
|
| ICC/IF |
1/100.
|
| Notes |
|---|
|
IP
1/60. |
|
ICC/IF
1/100. |
Target
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Function
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
Sequence similarities
Belongs to the PCNA family. -
Post-translational
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
Cellular localization
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
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Database links
- Entrez Gene: 5111 Human
- Entrez Gene: 18538 Mouse
- Entrez Gene: 25737 Rat
- Omim: 176740 Human
- SwissProt: P12004 Human
- SwissProt: P17918 Mouse
- SwissProt: P04961 Rat
- Unigene: 147433 Human
see all -
Alternative names
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)
Immunocytochemical analysis of 100% methanol-fixed NIH/3T3 (mouse embryonic fibroblast) cells labeling PCNA (green) using ab252848 at 1/100 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2µg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10µg/ml) followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (2µg/ml) (Red). Confocal image showing nuclear staining in NIH/3T3 cell line. The nuclear counterdstain was DAPI (Blue).
-ve control 1: ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).
-ve control 2: ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).
This image was produced by the same antibody in a different buffer formulation containing PBS, BSA and sodium azide (ab252848).
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![Immunoprecipitation - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)](https://www.abcam.com/ps/products/255/ab255842/Images/ab255842-397078-anti-pcna-antibody-16d10-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)PCNA was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate using ab252848 at 1/60 dilution (2µg in 0.35µg lysates). Western blot was performed on the immunoprecipitate using ab252848 at 1/1000 dilution (0.35µg/ml) followed by anti-Rat IgG H&L (HRP) (ab205720) secondary at 1/5000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10 µg.
Lane 2: ab252848 IP in NIH/3T3 whole cell lysate.
Lane 3: Rat monoclonal IgG instead of ab252848 in NIH/3T3 whole cell lysate.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This image was produced by the same antibody in a different buffer formulation containing PBS, BSA and sodium azide (ab252848).
-
![Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)](https://www.abcam.com/ps/products/255/ab255842/Images/ab255842-397076-anti-pcna-antibody-16d10-immunocytochemistry-c6-cells.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)Immunocytochemical analysis of 100% methanol-fixed C6 (rat glial tumor glial cell) cells labeling PCNA (green) using ab252848 at 1/100 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2µg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10µg/ml) followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (2µg/ml) (Red). Confocal image showing nuclear staining in C6 cell line. The nuclear counterdstain was DAPI (Blue).
-ve control 1: ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).
-ve control 2: ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).
This image was produced by the same antibody in a different buffer formulation containing PBS, BSA and sodium azide (ab252848)
-
![Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)](https://www.abcam.com/ps/products/255/ab255842/Images/ab255842-397077-anti-pcna-antibody-16d10-immunocytochemistry-hela-cells.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)Immunocytochemical analysis of 100% methanol-fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PCNA (green) using ab252848 at 1/100 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2µg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10µg/ml) followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (2µg/ml) (Red). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterdstain was DAPI (Blue).
-ve control 1: ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).
-ve control 2: ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).
This image was produced by the same antibody in a different buffer formulation containing PBS, BSA and sodium azide (ab252848)
-
![Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)](https://www.abcam.com/ps/products/255/ab255842/Images/ab255842-5-benefits-of-recombinant-antibodies.png)
Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab255842 has been referenced in 1 publication.
- Spada F et al. DNMT1 but not its interaction with the replication machinery is required for maintenance of DNA methylation in human cells. J Cell Biol 176:565-71 (2007). PubMed: 17312023
