Anti-PCNA antibody (ab152112)
Key features and details
- Rabbit polyclonal to PCNA
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PCNA antibody
See all PCNA primary antibodies -
Description
Rabbit polyclonal to PCNA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-253 of PCNA (Uniprot: P12004).
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Positive control
- Jurkat, Raji, 293T, A431, HeLa, HepG2, H1299, HCT116, MCF7, NT2D1, PC3, U87 MG, NIH 3T3, JC, BCL1, PC12, A549 and SiHa whole cell lysates; HeLa cells; Human KYSE170 xenograft and Human breast carcinoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
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| IHC-P | (2) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.
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| ICC/IF |
1/100 - 1/1000.
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| IP |
1/100 - 1/500.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8. |
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ICC/IF
1/100 - 1/1000. |
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IP
1/100 - 1/500. |
Target
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Function
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
Sequence similarities
Belongs to the PCNA family. -
Post-translational
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
Cellular localization
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
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Database links
- Entrez Gene: 5111 Human
- Entrez Gene: 18538 Mouse
- Entrez Gene: 692192 Pig
- Entrez Gene: 25737 Rat
- Omim: 176740 Human
- SwissProt: P12004 Human
- SwissProt: P17918 Mouse
- SwissProt: P04961 Rat
see all -
Alternative names
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
Images
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Western blot - Anti-PCNA antibody (ab152112)All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1 : HCT116 whole cell lysate at 15 µg
Lane 2 : HCT116 whole cell lysate at 10 µg
Lane 3 : HCT116 whole cell lysate at 5 µg
Lane 4 : HCT116 whole cell lysate at 1 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Immunoprecipitation - Anti-PCNA antibody (ab152112)PCBP2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts with 5 µg ab152112. Western blot was performed from the immunoprecipitate using ab152112.
Lane 1: PCPB2 protein only.
Lane 2: IgG instead of ab152112 in HeLa whole cell extract.
Lane 3: ab152112 IP in HeLa whole cell extract.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Paraffin-embedded mouseliver tissue stained for PCNA using ab152112 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Human KYSE170 xenograft tissue labeling PCNA with ab152112 at 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Rat hind brain tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PCNA with ab152112 at 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab152112)Immunofluorescent analysis of methanol-fixed HeLa cells labeling PCNA with ab152112 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab152112)Immunofluorescent analysis of HeLa (Human epithelial cells from cervix adenocarcinoma cell line) labeling PCNA with ab152112 at 1/500 dilution.
Counterstain is Hoechst 33342 (blue).
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Western blot - Anti-PCNA antibody (ab152112)All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : A431 whole cell lysate
Lane 5 : HeLa whole cell lyate
Lane 6 : HepG2 whole cell lysate
Lane 7 : H1299 whole cell lysate
Lane 8 : HCT116 whole cell lysate
Lane 9 : MCF7 whole cell lysate
Lane 10 : NT2D1 whole cell lysate
Lane 11 : PC3 whole cell lysate
Lane 12 : U87 MG whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-PCNA antibody (ab152112)All lanes : Anti-PCNA antibody (ab152112) at 1/1000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-PCNA antibody (ab152112)Anti-PCNA antibody (ab152112) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-PCNA antibody (ab152112)All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : NIH 3T3 whole cell lysate
Lane 3 : PC12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-PCNA antibody (ab152112)Anti-PCNA antibody (ab152112) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab152112 has been referenced in 25 publications.
- Jin M et al. Circ_0011292 knockdown mitigates progression and drug resistance in PTX-resistant non-small-cell lung cancer cells by regulating miR-433-3p/CHEK1 axis. Thorac Cancer 13:1276-1288 (2022). PubMed: 35348291
- Xu B et al. Circ_0017274 acts on miR-637/CDX2 axis to facilitate cisplatin resistance in gastric cancer. Clin Exp Pharmacol Physiol 49:1105-1115 (2022). PubMed: 35748299
- Gustorff C et al. GABAB Receptor-Mediated Impairment of Intermediate Progenitor Maturation During Postnatal Hippocampal Neurogenesis of Newborn Rats. Front Cell Neurosci 15:651072 (2021). PubMed: 34421540
- Wang J et al. Knockdown of long non-coding RNA PVT1 induces apoptosis of fibroblast-like synoviocytes through modulating miR-543-dependent SCUBE2 in rheumatoid arthritis. J Orthop Surg Res 15:142 (2020). PubMed: 32293498
- Feng C et al. Tumor-Suppressive MicroRNA-216b Binds to TPX2, Activating the p53 Signaling in Human Cutaneous Squamous Cell Carcinoma. Mol Ther Nucleic Acids 20:186-195 (2020). PubMed: 32169806