Recombinant Anti-PCNA antibody [BLR075G] (ab265609)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR075G] to PCNA
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PCNA antibody [BLR075G]
See all PCNA primary antibodies -
Description
Rabbit monoclonal [BLR075G] to PCNA -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PCNA aa 225-261. The exact sequence is proprietary. NP_002583.1
Database link: P12004 -
Positive control
- WB: A549, Jurkat, HEK-293T, RKO, K562, MCF7, HeLa and U-2 OS whole cell lysate. IHC-P: Human prostate carcinoma, ovarian carcinoma tissue, breast carcinoma. ICC: OVCAR-3 cells.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
BLR075G -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab265609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/100 - 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with ICC staining protocol. |
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/1000. Predicted molecular weight: 29 kDa.
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| Notes |
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ICC
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with ICC staining protocol. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000. Predicted molecular weight: 29 kDa. |
Target
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Function
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
Sequence similarities
Belongs to the PCNA family. -
Post-translational
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
Cellular localization
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
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Database links
- Entrez Gene: 5111 Human
- Omim: 176740 Human
- SwissProt: P12004 Human
- Unigene: 147433 Human
- Unigene: 728886 Human
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Alternative names
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab265609)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling PCNA with ab265609 at 1/250 dilution. Detection: DAB.
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Western blot - Anti-PCNA antibody (ab265609)All lanes : Anti-PCNA antibody [BLR075G] (ab265609) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lane 4 : RKO whole cell lysate
Lane 5 : K562 whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : U-2 OS whole cell lysate
Lane 9 : TCMK whole cell lysate
Lane 10 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 29 kDa
Exposure time: 30 secondsCells prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab265609)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling PCNA with ab265609 at 1/250 dilution. Detection: DAB.
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Immunocytochemistry - Anti-PCNA antibody (ab265609)Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling PCNA with ab265609 at 1/250 dilution. Detection: DAB.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] (ab265609)](https://www.abcam.com/ps/products/265/ab265609/Images/ab265609-464724-AntiPCNA-antibody-BLR075G-immunohistochemistry-breast-carcinoma-human.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] (ab265609)Paraffin embedded human breast carcinoma tissue stained for PCNA using ab265609 at 1/500 dilution in immunohistochemical analysis. Secondary antibody used HRP-conjugated goat anti-rabbit IgG. Counterstain: DAPI.
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![Anti-PCNA antibody [BLR075G] (ab265609)](https://www.abcam.com/ps/products/265/ab265609/Images/ab265609-5-benefits-of-recombinant-antibodies.png)
Anti-PCNA antibody [BLR075G] (ab265609)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab265609 has been referenced in 5 publications.
- Li YK et al. Validation of ESM1 Related to Ovarian Cancer and the Biological Function and Prognostic Significance. Int J Biol Sci 19:258-280 (2023). PubMed: 36594088
- Chen S et al. Oxygenized Low-Density Lipoprotein-Induced ASMC Dysregulation Depends on circ_0000345-Mediated Regulatory Mechanism. J Atheroscler Thromb 29:1849-1863 (2022). PubMed: 36171087
- Tang M et al. Identification of Biomarkers Related to CD8+ T Cell Infiltration With Gene Co-expression Network in Lung Squamous Cell Carcinoma. Front Cell Dev Biol 9:606106 (2021). PubMed: 33816462
- Yang Y et al. LncRNA NEAT1 Promotes Inflammatory Response in Sepsis via the miR-31-5p/POU2F1 Axis. Inflammation 44:1518-1528 (2021). PubMed: 33710444
- Qian H et al. Immunity-Related Gene Signature Identifies Subtypes Benefitting From Adjuvant Chemotherapy or Potentially Responding to PD1/PD-L1 Blockage in Pancreatic Cancer. Front Cell Dev Biol 9:682261 (2021). PubMed: 34249934