Recombinant Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)](https://www.abcam.com/ps/products/272/ab272076/Images/ab272076-1-ab265609366205AntiPCNAantibodyimmunohistochemistryprostatecarcinomahuman.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR075G] to PCNA - BSA and Azide free
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PCNA antibody [BLR075G] - BSA and Azide free
See all PCNA primary antibodies -
Description
Rabbit monoclonal [BLR075G] to PCNA - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PCNA aa 225-261. The exact sequence is proprietary. NP_002583.1
Database link: P12004 -
Positive control
- WB: A549, Jurkat, HEK-293T, RKO, K562, MCF7, HeLa and U-2 OS whole cell lysate. IHC-P: Human prostate carcinoma and ovarian carcinoma tissue. ICC: OVCAR-3 cells.
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General notes
ab272076 is the carrier-free version of ab265609.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
BLR075G -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC
Use at an assay dependent concentration. |
Target
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Function
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
Sequence similarities
Belongs to the PCNA family. -
Post-translational
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
Cellular localization
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
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Database links
- Entrez Gene: 5111 Human
- Omim: 176740 Human
- SwissProt: P12004 Human
- Unigene: 147433 Human
- Unigene: 728886 Human
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Alternative names
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling PCNA with ab265609 at 1/250 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265609).
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![Western blot - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)](https://www.abcam.com/ps/products/272/ab272076/Images/ab272076-468239-ab265609-anti-pcna-antibody-western-blot.jpg)
Western blot - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)All lanes : Anti-PCNA antibody [BLR075G] (ab265609) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lane 4 : RKO whole cell lysate
Lane 5 : K562 whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : U-2 OS whole cell lysate
Lane 9 : TCMK whole cell lysate
Lane 10 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 29 kDa
Exposure time: 30 secondsDetection: Chemiluminescence.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265609).
Lysates prepared using NETN lysis buffer.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)](https://www.abcam.com/ps/products/272/ab272076/Images/ab272076-2-ab265609366204AntiPCNAantibodyimmunohistochemistryovariancarcinomahuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling PCNA with ab265609 at 1/250 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265609).
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![Immunocytochemistry - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)](https://www.abcam.com/ps/products/272/ab272076/Images/ab272076-3-ab265609366203AntiPCNAantibodyimmunocytochemistryovcar3human.jpg)
Immunocytochemistry - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling PCNA with ab265609 at 1/250 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265609).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)](https://www.abcam.com/ps/products/272/ab272076/Images/ab272076-468240-ab265609-anti-pcna-antibody-human-breast-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265609).
Paraffin embedded human breast carcinoma tissue stained for PCNA using ab265609 at 1/500 dilution in immunohistochemical analysis. Secondary antibody used HRP-conjugated goat anti-rabbit IgG. Counterstain: DAPI.
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![Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)](https://www.abcam.com/ps/products/272/ab272076/Images/ab272076-4-benefits-of-recombinant-antibodies.png)
Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab272076 has not yet been referenced specifically in any publications.