Recombinant Anti-PCNA antibody [EPR3822] (ab92729)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)](https://www.abcam.com/ps/products/92/ab92729/Images/ab92729-107938-anti-pcna-antibody-epr3822-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3822] to PCNA
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PCNA antibody [EPR3822]
See all PCNA primary antibodies -
Description
Rabbit monoclonal [EPR3822] to PCNA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human PCNA aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, HepG2 and 293T cell lysates; Human colonic adenocarcinoma tissue; Human tonsil tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3822 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IHC-P |
| Notes |
|---|
Application notes
Flow Cyt: 1/10.
IHC-P: 1/50 - 1/100. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
WB: 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
Is unsuitable for ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
WB: 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
Is unsuitable for ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
Sequence similarities
Belongs to the PCNA family. -
Post-translational
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
Cellular localization
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
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Database links
- Entrez Gene: 5111 Human
- Entrez Gene: 18538 Mouse
- Omim: 176740 Human
- SwissProt: P12004 Human
- SwissProt: P17918 Mouse
- Unigene: 147433 Human
- Unigene: 728886 Human
- Unigene: 7141 Mouse
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Alternative names
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)
ab92729, at 1/50 dilution, staining PCNA in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemisty.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)](https://www.abcam.com/ps/products/92/ab92729/Images/ab92729-107937-anti-pcna-antibody-epr3822-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)ab92729, at 1/50 dilution, staining PCNA in paraffin embedded Human tonsil tissue by Immunohistochemisty.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-PCNA antibody [EPR3822] (ab92729)](https://www.abcam.com/ps/products/92/ab92729/Images/ab92729-107939-anti-pcna-antibody-epr3822-western-blot.jpg)
Western blot - Anti-PCNA antibody [EPR3822] (ab92729)All lanes : Anti-PCNA antibody [EPR3822] (ab92729) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 29 kDa -
![Anti-PCNA antibody [EPR3822] (ab92729)](https://www.abcam.com/ps/products/92/ab92729/Images/ab92729-4-benefits-of-recombinant-antibodies.png)
Anti-PCNA antibody [EPR3822] (ab92729)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab92729 has been referenced in 8 publications.
- Liu W et al. Chemopreventive efficacy of salvianolic acid B phospholipid complex loaded nanoparticles against experimental oral carcinogenesis: implication of sustained drug release. Ann Transl Med 10:244 (2022). PubMed: 35402586
- Liu X et al. CREB1 acts via the miR-922/ARID2 axis to enhance malignant behavior of liver cancer cells. Oncol Rep 45:N/A (2021). PubMed: 33786634
- Qu M et al. Uncovering the action mechanism of homoharringtonine against colorectal cancer by using network pharmacology and experimental evaluation. Bioengineered 12:12940-12953 (2021). PubMed: 34847838
- Williams TL et al. Making urinary extracellular vesicles a clinically tractable source of biomarkers for inherited tubulopathies using a small volume precipitation method: proof of concept. J Nephrol 33:383-386 (2020). PubMed: 31586298
- Tohmé R et al. Direct activation of PP2A for the treatment of tyrosine kinase inhibitor-resistant lung adenocarcinoma. JCI Insight 4:N/A (2019). PubMed: 30830869
- McClinch K et al. Small-Molecule Activators of Protein Phosphatase 2A for the Treatment of Castration-Resistant Prostate Cancer. Cancer Res 78:2065-2080 (2018). PubMed: 29358171
- Hamajima N et al. Increased Protein Stability of CDKN1C Causes a Gain-of-Function Phenotype in Patients with IMAGe Syndrome. PLoS One 8:e75137 (2013). WB ; Human . PubMed: 24098681
- Hanel ML et al. Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) is a dynamic nuclear and sarcomeric protein. Differentiation 81:107-18 (2011). WB . PubMed: 20970242