Anti-PCNA antibody [PC10] (ab265585)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab265585)](https://www.abcam.com/ps/products/265/ab265585/Images/ab265585-366242-anti-Pcna-antibody-immunohistochemistry-colon-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab265585)")
Key features and details
- Mouse monoclonal [PC10] to PCNA
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-PCNA antibody [PC10]
See all PCNA primary antibodies -
Description
Mouse monoclonal [PC10] to PCNA -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Rat PCNA.
Database link: P12004 -
Positive control
- IHC-P: Human colon carcinoma tissue and mouse colon carcinoma tissue. ICC/IF: H226 cells. WB: TCMK-1, NIH/3T3, HeLa and jurkat cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
PC10 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab265585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 29 kDa.
|
|
| IP |
Use at an assay dependent concentration.
20 µl/1 mg lysate |
|
| IHC-P |
1/100 - 1/500.
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
|
| ICC/IF |
1/100 - 1/500.
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections |
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 29 kDa. |
|
IP
Use at an assay dependent concentration. 20 µl/1 mg lysate |
|
IHC-P
1/100 - 1/500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
|
ICC/IF
1/100 - 1/500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections |
Target
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Function
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
Sequence similarities
Belongs to the PCNA family. -
Post-translational
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
Cellular localization
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
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Database links
- Entrez Gene: 5111 Human
- Entrez Gene: 18538 Mouse
- Omim: 176740 Human
- SwissProt: P12004 Human
- SwissProt: P17918 Mouse
- Unigene: 147433 Human
- Unigene: 728886 Human
- Unigene: 7141 Mouse
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Alternative names
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab265585)
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PCNA using ab265585 at 1/100 dilution in immunohistochemical analysis. Substrate: DAB
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![Immunoprecipitation - Anti-PCNA antibody [PC10] (ab265585)](https://www.abcam.com/ps/products/265/ab265585/Images/ab265585-366246-anti-Pcna-antibody-immunoprecipitation-tcmk1-lysate.jpg)
Immunoprecipitation - Anti-PCNA antibody [PC10] (ab265585)PCNA was immunoprecipitated from 1 mg TCMK-1 cell lysate using ab265585 at 20 µl/mg lysate. Western blot was performed on the immunoprecipitate using ab265585 at 1/1000 dilution.
Lane 1: ab265585 IP in TCMK-1 whole cell lysate.
Lane 2: Control IgG. -
![Western blot - Anti-PCNA antibody [PC10] (ab265585)](https://www.abcam.com/ps/products/265/ab265585/Images/ab265585-366245-anti-Pcna-antibody-western-blot--tcmk1-lysate.jpg)
Western blot - Anti-PCNA antibody [PC10] (ab265585)All lanes : Anti-PCNA antibody [PC10] (ab265585) at 1/1000 dilution
Lane 1 : TCMK-1 whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 1 second -
![Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [PC10] (ab265585)](https://www.abcam.com/ps/products/265/ab265585/Images/ab265585-366244-anti-Pcna-antibody-immunocytochemistry-h226-cells.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [PC10] (ab265585)Formalin-fixed, paraffin-embedded H226 cells stained for PCNA using ab265585 at 1/100 dilution in ICC/IF analysis. Substrate: DAB.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab265585)](https://www.abcam.com/ps/products/265/ab265585/Images/ab265585-366243-anti-Pcna-antibody-immunohistochemistry-mouse-colon-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab265585)Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for PCNA using ab265585 at 1/100 dilution in immunohistochemical analysis. Substrate: DAB
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab265585 has been referenced in 1 publication.
- Zhang C et al. Asiaticoside Suppresses Gastric Cancer Progression and Induces Endoplasmic Reticulum Stress through the miR-635/HMGA1 Axis. J Immunol Res 2022:1917585 (2022). PubMed: 35692504
