Anti-PCSK2 antibody (ab3533)
Key features and details
- Rabbit polyclonal to PCSK2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PCSK2 antibody
See all PCSK2 primary antibodies -
Description
Rabbit polyclonal to PCSK2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig
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Immunogen
Synthetic peptide corresponding to Mouse PCSK2 aa 600-700.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/20 - 1/200.
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| WB |
Use a concentration of 2 µg/ml.
This antibody detects an ~65 kDa protein representing Proprotein 2 from alpha TC 6 cell extract. |
| Notes |
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ICC/IF
1/20 - 1/200. |
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WB
Use a concentration of 2 µg/ml. This antibody detects an ~65 kDa protein representing Proprotein 2 from alpha TC 6 cell extract. |
Target
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Function
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. -
Sequence similarities
Belongs to the peptidase S8 family. Furin subfamily. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules. - Information by UniProt
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Database links
- Entrez Gene: 281968 Cow
- Entrez Gene: 5126 Human
- Entrez Gene: 18549 Mouse
- Entrez Gene: 445533 Pig
- Entrez Gene: 25121 Rat
- Omim: 162151 Human
- SwissProt: Q9GLR0 Cow
- SwissProt: P16519 Human
see all -
Alternative names
- KEX2 like endoprotease 2 antibody
- KEX2-like endoprotease 2 antibody
- NEC 2 antibody
see all
Images
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Western blot - Anti-PCSK2 antibody (ab3533)Anti-PCSK2 antibody (ab3533) at 1/500 dilution + Mouse cerebellum cell lysate at 25 µg
Observed band size: 65 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody (ab3533)Immunocytochemistry/Immunofluorescent analysis of PCSK2 (green) showing staining in the cytoplasm of HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody (ab3533)Immunocytochemistry/Immunofluorescent analysis of PCSK2 (green) showing staining in the cytoplasm of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab3533 has been referenced in 5 publications.
- Ducuing H et al. SlitC-PlexinA1 mediates iterative inhibition for orderly passage of spinal commissural axons through the floor plate. Elife 9:N/A (2020). PubMed: 33345773
- Kreutzberger AJB et al. Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2. Elife 9:N/A (2020). PubMed: 33164744
- Wu S et al. Apatinib inhibits tumor growth and angiogenesis in PNET models. Endocr Connect N/A:N/A (2018). PubMed: 30557852
- Du W et al. HID-1 is required for homotypic fusion of immature secretory granules during maturation. Elife 5:N/A (2016). WB, IF ; Mouse . PubMed: 27751232
- Gagnon J et al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009). PubMed: 18706454
