Recombinant Anti-PCSK2 antibody [EPR23578-19] (ab274418)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23578-19] to PCSK2
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), mIHC, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PCSK2 antibody [EPR23578-19]
See all PCSK2 primary antibodies -
Description
Rabbit monoclonal [EPR23578-19] to PCSK2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), mIHC, WBmore details
Unsuitable for: IHC-Fr or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: TT whole cell lysate; Human brain tissue lysate. IHC-P: Human pancreas, colon and adrenal gland tissue. ICC/IF: TT cells. Flow Cyt (intra): TT cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23578-19 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab274418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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Flow Cyt (Intra) |
1/500.
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mIHC |
1/2000.
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WB |
1/1000. Detects a band of approximately 55, 70 kDa (predicted molecular weight: 71 kDa).
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Notes |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/500. |
mIHC
1/2000. |
WB
1/1000. Detects a band of approximately 55, 70 kDa (predicted molecular weight: 71 kDa). |
Target
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Function
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. -
Sequence similarities
Belongs to the peptidase S8 family. Furin subfamily. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules. - Information by UniProt
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Database links
- Entrez Gene: 5126 Human
- Omim: 162151 Human
- SwissProt: P16519 Human
- Unigene: 315186 Human
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Alternative names
- KEX2 like endoprotease 2 antibody
- KEX2-like endoprotease 2 antibody
- NEC 2 antibody
see all
Images
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Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (ab274418; red; Opal™690) at 1:2000 (0.263 μg/ml) [Panel B], anti-C11B2/CYP11B2 stained on zona glomerulosa (ab249476; cyan; Opal™520) at 1:500 (4.478 μg/ml) [Panel C], and anti-SULT2A1 stained on zona reticularis (ab240333; yellow; Opal™570) at 1:500 (4.504 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of ab274418, ab249476, and ab240333 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
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Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (ab274418; gray; Opal™690) at 1:2000 (0.263 μg/ml) [Panel B], anti-CYP11A1 stained on adrenal cortex (ab272494; green; Opal™520) at 1:10000 (0.053 μg/ml) [Panel C], and anti-Collagen VI stained on extracellular matrix (ab182744; red; Opal™570) at 1:1000 (1.518 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of ab274418, ab272494, and ab182744 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
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Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (ab274418; gray; Opal™690) at 1:2000 (0.263 μg/ml) [Panel B], anti-CYP11A1 stained on adrenal cortex (ab272494; green; Opal™520) at 1:10000 (0.053 μg/ml) [Panel C], and anti-Neuropeptide Y stained on chromaffin cells (ab221145; red; Opal™570) at 1:2000 (0.279 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of ab274418, ab272494, and ab221145 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
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Anti-PCSK2 antibody [EPR23578-19] (ab274418) at 1/1000 dilution + Human brain tissue lysate at 40 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
55-kDa PC2 is observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 8557169, 14693708).
This blot was developed using a higher sensitivity ECL substrate. -
Anti-PCSK2 antibody [EPR23578-19] (ab274418) at 1/1000 dilution + TT (human thyroid carcinoma epithelial cell) whole cell lysate at 40 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 71 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 37 seconds.
70-kDa pro-PC2 is observed. The molecular weight observed is consistent with what has been described in the literature (PMID:8557169, 14693708).
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized TT (Human thyroid carcinoma epithelial cell) cells labelling PCSK2 with ab274418 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized TT cells labelling PCSK2 with ab274418 at 1/100 (5.25 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic staining in TT cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human adrenal gland. The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on myenteric neurons of human colon (PMID: 7829629). The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human pancreatic islets (PMID: 8916141, PMID: 7829629). The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND. RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab274418 has not yet been referenced specifically in any publications.