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    products/primary-antibodies/pcsk2-antibody-epr23578-19-ab274418.pdf

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Signal Transduction Growth Factors/Hormones Insulin / Insulin-like
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RecombinantRabMAb

Recombinant Anti-PCSK2 antibody [EPR23578-19] (ab274418)

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  • SDS
  • Certificate of Compliance
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Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
  • Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
  • Flow Cytometry (Intracellular) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
  • Anti-PCSK2 antibody [EPR23578-19] (ab274418)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23578-19] to PCSK2
  • Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-PCSK2 antibody [EPR23578-19]
    See all PCSK2 primary antibodies
  • Description

    Rabbit monoclonal [EPR23578-19] to PCSK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details
    Unsuitable for: IHC-Fr or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: TT whole cell lysate; Human brain tissue lysate. IHC-P: Human pancreas, colon and adrenal gland tissue. ICC/IF: TT cells. Flow Cyt (intra): TT cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23578-19
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Serine protease
    • Proprotein Convertases

Associated products

  • Alternative Versions

    • Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab274418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 55, 70 kDa (predicted molecular weight: 71 kDa).
Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 55, 70 kDa (predicted molecular weight: 71 kDa).
Application notes
Is unsuitable for IHC-Fr or IP.

Target

  • Function

    Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues.
  • Sequence similarities

    Belongs to the peptidase S8 family. Furin subfamily.
  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
  • Target information above from: UniProt accession P16519 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5126 Human
    • Omim: 162151 Human
    • SwissProt: P16519 Human
    • Unigene: 315186 Human
    • Alternative names

      • KEX2 like endoprotease 2 antibody
      • KEX2-like endoprotease 2 antibody
      • NEC 2 antibody
      • NEC2 antibody
      • NEC2_HUMAN antibody
      • Neuroendocrine convertase 2 antibody
      • PC2 antibody
      • PCSK2 antibody
      • Prohormone convertase 2 antibody
      • Proprotein convertase 2 antibody
      • Proprotein convertase subtilisin/kexin type 2 antibody
      • SPC2 antibody
      see all

    Images

    • Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
      Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
      Anti-PCSK2 antibody [EPR23578-19] (ab274418) at 1/1000 dilution + Human brain tissue lysate at 40 µg

      Secondary
      VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 71 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      55-kDa PC2 is observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 8557169, 14693708).
      This blot was developed using a higher sensitivity ECL substrate.

    • Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
      Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
      Anti-PCSK2 antibody [EPR23578-19] (ab274418) at 1/1000 dilution + TT (human thyroid carcinoma epithelial cell) whole cell lysate at 40 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

      Predicted band size: 71 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 37 seconds.

      70-kDa pro-PC2 is observed. The molecular weight observed is consistent with what has been described in the literature (PMID:8557169, 14693708).

       

    • Flow Cytometry (Intracellular) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
      Flow Cytometry (Intracellular) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized TT (Human thyroid carcinoma epithelial cell) cells labelling PCSK2 with ab274418 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

      A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
      Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody [EPR23578-19] (ab274418)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized TT cells labelling PCSK2 with ab274418 at 1/100 (5.25 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic staining in TT cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)

      Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human adrenal gland. The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on myenteric neurons of human colon (PMID: 7829629). The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human pancreatic islets (PMID: 8916141, PMID: 7829629). The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND. RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-PCSK2 antibody [EPR23578-19] (ab274418)
      Anti-PCSK2 antibody [EPR23578-19] (ab274418)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab274418? Please let us know so that we can cite the reference in this datasheet.

    ab274418 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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