Recombinant Anti-PCSK2 antibody [EPR23578-19] (ab274418)
![Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418)](https://www.abcam.com/ps/products/274/ab274418/Images/ab274418-7-anti-pcsk2-antibody-epr2357819-western-blot-lysate.jpg "Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23578-19] to PCSK2
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PCSK2 antibody [EPR23578-19]
See all PCSK2 primary antibodies -
Description
Rabbit monoclonal [EPR23578-19] to PCSK2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details
Unsuitable for: IHC-Fr or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: TT whole cell lysate; Human brain tissue lysate. IHC-P: Human pancreas, colon and adrenal gland tissue. ICC/IF: TT cells. Flow Cyt (intra): TT cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23578-19 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab274418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
1/100.
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| Flow Cyt (Intra) |
1/500.
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| WB |
1/1000. Detects a band of approximately 55, 70 kDa (predicted molecular weight: 71 kDa).
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| Notes |
|---|
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/100. |
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Flow Cyt (Intra)
1/500. |
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WB
1/1000. Detects a band of approximately 55, 70 kDa (predicted molecular weight: 71 kDa). |
Target
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Function
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. -
Sequence similarities
Belongs to the peptidase S8 family. Furin subfamily. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules. - Information by UniProt
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Database links
- Entrez Gene: 5126 Human
- Omim: 162151 Human
- SwissProt: P16519 Human
- Unigene: 315186 Human
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Alternative names
- KEX2 like endoprotease 2 antibody
- KEX2-like endoprotease 2 antibody
- NEC 2 antibody
see all
Images
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Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418)Anti-PCSK2 antibody [EPR23578-19] (ab274418) at 1/1000 dilution + Human brain tissue lysate at 40 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
55-kDa PC2 is observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 8557169, 14693708).
This blot was developed using a higher sensitivity ECL substrate. -
![Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418)](https://www.abcam.com/ps/products/274/ab274418/Images/ab274418-6-anti-pcsk2-antibody-epr2357819-western-blot-carcinoma.jpg)
Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418)Anti-PCSK2 antibody [EPR23578-19] (ab274418) at 1/1000 dilution + TT (human thyroid carcinoma epithelial cell) whole cell lysate at 40 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 71 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 37 seconds.
70-kDa pro-PC2 is observed. The molecular weight observed is consistent with what has been described in the literature (PMID:8557169, 14693708).
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![Flow Cytometry (Intracellular) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)](https://www.abcam.com/ps/products/274/ab274418/Images/ab274418-5-anti-pcsk2-antibody-epr2357819-flow-cytometry-tt.jpg)
Flow Cytometry (Intracellular) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized TT (Human thyroid carcinoma epithelial cell) cells labelling PCSK2 with ab274418 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody [EPR23578-19] (ab274418)](https://www.abcam.com/ps/products/274/ab274418/Images/ab274418-4-anti-pcsk2-antibody-epr2357819-immunocytochemistry-tt.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody [EPR23578-19] (ab274418)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized TT cells labelling PCSK2 with ab274418 at 1/100 (5.25 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic staining in TT cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)](https://www.abcam.com/ps/products/274/ab274418/Images/ab274418-3-anti-pcsk2-antibody-epr2357819-immunohistochemistry-human-adrenal-gland.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human adrenal gland. The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)](https://www.abcam.com/ps/products/274/ab274418/Images/ab274418-2-anti-pcsk2-antibody-epr2357819-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on myenteric neurons of human colon (PMID: 7829629). The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)](https://www.abcam.com/ps/products/274/ab274418/Images/ab274418-1-anti-pcsk2-antibody-epr2357819-immunohistochemistry-human-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (ab274418)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human pancreatic islets (PMID: 8916141, PMID: 7829629). The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND. RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-PCSK2 antibody [EPR23578-19] (ab274418)](https://www.abcam.com/ps/products/274/ab274418/Images/ab274418-8-benefits-of-recombinant-antibodies.png)
Anti-PCSK2 antibody [EPR23578-19] (ab274418)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab274418 has not yet been referenced specifically in any publications.