Recombinant Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
![Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)](https://www.abcam.com/ps/products/181/ab181142/Images/ab181142-253922-anti-pcsk9-antibody-epr76272-western-blot.jpg "Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7627(2)] to PCSK9
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PCSK9 antibody [EPR7627(2)]
See all PCSK9 primary antibodies -
Description
Rabbit monoclonal [EPR7627(2)] to PCSK9 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7627(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30 - 1/50.
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| WB |
1/1000 - 1/10000. Detects a band of approximately 62, 78 kDa (predicted molecular weight: 74 kDa).
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| Notes |
|---|
|
IP
1/30 - 1/50. |
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WB
1/1000 - 1/10000. Detects a band of approximately 62, 78 kDa (predicted molecular weight: 74 kDa). |
Target
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Function
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. -
Tissue specificity
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. -
Involvement in disease
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant. -
Sequence similarities
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain. -
Post-translational
modificationsThe soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 255738 Human
- Omim: 607786 Human
- SwissProt: Q8NBP7 Human
- Unigene: 18844 Human
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Alternative names
- Convertase subtilisin/kexin type 9 preproprotein antibody
- FH3 antibody
- HCHOLA3 antibody
see all
Images
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Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/5000 dilution (purified) + BxPC-3 whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Predicted band size: 74 kDa
Observed band size: 62,78 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)](https://www.abcam.com/ps/products/181/ab181142/Images/ab181142-253921-anti-pcsk9-antibody-epr76272-western-blot.jpg)
Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)All lanes : Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/20000 dilution (purified)
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 62,78 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)](https://www.abcam.com/ps/products/181/ab181142/Images/ab181142-253848-anti-pcsk9-antibody-epr76272-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)ab181142 (purified) at 1/50 immunoprecipitating PCSK9 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10µg)
Lane 2 (+): ab181142 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181142 in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)](https://www.abcam.com/ps/products/181/ab181142/Images/ab181142-214713-anti-pcsk9-antibody-epr76272-western-blot.jpg)
Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)All lanes : Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/10000 dilution (unpurified)
Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 74 kDa
Observed band size: 62,78 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)](https://www.abcam.com/ps/products/181/ab181142/Images/ab181142-214711-anti-pcsk9-antibody-epr76272-western-blot.jpg)
Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/2000 dilution (unpurified) + Human fetal liver lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 74 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)](https://www.abcam.com/ps/products/181/ab181142/Images/ab181142-214714-anti-pcsk9-antibody-epr76272-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)Western blot analysis of PCSK9 in HepG2 cell lysate immunoprecipitated using unpurified ab181142 at a dilution of 1/50.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
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![Anti-PCSK9 antibody [EPR7627(2)] (ab181142)](https://www.abcam.com/ps/products/181/ab181142/Images/ab181142-7-benefits-of-recombinant-antibodies.png)
Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (10)
ab181142 has been referenced in 10 publications.
- Xu M et al. Development of a novel, fully human, anti-PCSK9 antibody with potent hypolipidemic activity by utilizing phage display-based strategy. EBioMedicine 65:103250 (2021). PubMed: 33647772
- Zhong S et al. Acetaldehyde Dehydrogenase 2 regulates HMG-CoA reductase stability and cholesterol synthesis in the liver. Redox Biol 41:101919 (2021). PubMed: 33740503
- Huang Y et al. Hydrogen sulfide accumulates LDL receptor precursor via downregulating PCSK9 in HepG2 cells. Am J Physiol Cell Physiol 319:C1082-C1096 (2020). PubMed: 32938225
- Wu C et al. Design, synthesis, and biological evaluation of novel tetrahydroprotoberberine derivatives (THPBs) as proprotein convertase subtilisin/kexin type 9 (PCSK9) modulators for the treatment of hyperlipidemia. Acta Pharm Sin B 9:1216-1230 (2019). PubMed: 31867167
- Li HH et al. 23,24-Dihydrocucurbitacin B promotes lipid clearance by dual transcriptional regulation of LDLR and PCSK9. Acta Pharmacol Sin N/A:N/A (2019). PubMed: 31358898
- Yang X et al. Chitosan oligosaccharides enhance lipid droplets via down-regulation of PCSK9 gene expression in HepG2 cells. Exp Cell Res 366:152-160 (2018). Mouse . PubMed: 29548750
- Yu Q et al. Short-term use of atorvastatin affects glucose homeostasis and suppresses the expression of LDL receptors in the pancreas of mice. Mol Med Rep 18:2780-2788 (2018). PubMed: 30015940
- Xu X et al. PCSK9 regulates apoptosis in human lung adenocarcinoma A549 cells via endoplasmic reticulum stress and mitochondrial signaling pathways. Exp Ther Med 13:1993-1999 (2017). PubMed: 28565798
- Romagnuolo R et al. Roles of the low density lipoprotein receptor and related receptors in inhibition of lipoprotein(a) internalization by proprotein convertase subtilisin/kexin type 9. PLoS One 12:e0180869 (2017). PubMed: 28750079
- Gong J et al. Inhibition of FASN suppresses migration, invasion and growth in hepatoma carcinoma cells by deregulating the HIF-1a/IGFBP1 pathway. Int J Oncol 50:883-892 (2017). PubMed: 28197637