Recombinant Anti-PD-L1 antibody [EPR20529] (ab213480)
![Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)](https://www.abcam.com/ps/products/213/ab213480/Images/ab213480-482337-Rabbit-monoclonalEPR20529111-to-CD274-PDL1-western-blot-mouse.jpg "Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20529] to PD-L1
- Suitable for: ICC/IF, IP, WB
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-PD-L1 antibody [EPR20529]
See all PD-L1 primary antibodies -
Description
Rabbit monoclonal [EPR20529] to PD-L1 -
Host species
Rabbit -
Specificity
ab213480 is not suitable in IHC-FR for mouse samples
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Tested applications
Suitable for: ICC/IF, IP, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse placenta lysate, HEK mPDL1 cell lysate, whole cell lysate from RAW 264.7 cells treated with Interferon-gamma and whole cell lysate from MEF cells treated with Interferon-gamma. IP: Mouse placenta lysate. ICC/IF: RAW 264.7 cells treated with IFN-? (100 ng/ml) for 24 hours.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20529 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| IP |
1/30.
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| WB | (3) |
1/1000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 33 kDa).
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IP
1/30. |
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WB
1/1000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 33 kDa). |
Target
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Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane and Endomembrane system. - Information by UniProt
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Database links
- Entrez Gene: 60533 Mouse
- SwissProt: Q9EP73 Mouse
- Unigene: 245363 Mouse
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Alternative names
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
see all
Images
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Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)All lanes : Anti-PD-L1 antibody [EPR20529] (ab213480) at 1/5000 dilution
Lane 1 : Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : RAW264.7 treated with 100 ng/ml IFN gamma for 48 h, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 33 kDa
Observed band size: 40-60 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsThis WB was performed by using YCA-R20529(BF)-111A H3L2 YR120921PJ, 1:5000 dilution. Working concentration: 0.402 μg/ml.
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)](https://www.abcam.com/ps/products/213/ab213480/Images/ab213480-473349-anti-pdl1-antibody-epr20529-western-blot-human-mouse.jpg)
Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)This image is courtesy of an anonymous collaborator.All lanes : Anti-PD-L1 antibody [EPR20529] (ab213480) at 100 µg
Lane 1 : HEK mPDL1 cell lysate at 20 µg
Lane 2 : HEK hPDL1 cell lysate at 20 µg
Lane 3 : HEK CL7 at 20 µg
Lane 4 : RAW 264.7 Wild-type (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 100 µg
Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate treated with Interferon-gamma at 100 µg
Lane 6 : MEF Wild-type (Mouse embryonic fibroblast cell line) whole cell lysate at 100 µg
Lane 7 : MEF (Mouse embryonic fibroblast cell line) whole cell lysate treated with Interferon-gamma at 100 µg
Lane 8 : MEF mPDL1 KO cell lysate at 100 µg
Lane 9 : MEF mPDL1 KO treated with Interferon-gamma at 100 µg
Predicted band size: 33 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesThe mAb is specific for mouse mPDL1 and does not recognise hPDL1. As expected a specific 48kDa band is observed in RAW264.7 and MEFS cell extract after Interferon-gamma treatment. No bands are observed in RAW264.7 and MEFS WT and in MEFS KO with and without Interferon-gamma treatment.
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![Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR20529] (ab213480)](https://www.abcam.com/ps/products/213/ab213480/Images/ab213480-280108-anti-pd-l1-antibody-epr20529-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR20529] (ab213480)Ab213480 staining PD-L1 in RAW264.7 from Mouse macrophage cell line transformed with Abelson murine leukemia virus by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% Methanol. Samples were incubated with ab213480 at 0.4 μg/ml. AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 2 μg/ml. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)(ab196889) was used as counterstain at 2.5 μg/ml. DAPI used as nuclear counterstain. Confocal image showing membranous staining on RAW 264.7 cells treated with IFN-γ (100 ng/ml) for 24 hours.
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![Immunoprecipitation - Anti-PD-L1 antibody [EPR20529] (ab213480)](https://www.abcam.com/ps/products/213/ab213480/Images/ab213480-276143-anti-pd-l1-antibody-epr20529-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PD-L1 antibody [EPR20529] (ab213480)PD-L1 was immunoprecipitated from 0.35 mg of Mouse placenta lysate with ab213480 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab213480 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution.
Lane 1: Mouse placenta lysate, 10 µg (Input).
Lane 2: ab213480 IP in mouse placenta lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213480 in mouse placenta lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)](https://www.abcam.com/ps/products/213/ab213480/Images/ab213480-276146-anti-pd-l1-antibody-epr20529-western-blot.jpg)
Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)Anti-PD-L1 antibody [EPR20529] (ab213480) at 1/1000 dilution + Mouse placenta lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 40-60 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature. PMID: 15353579.
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![Anti-PD-L1 antibody [EPR20529] (ab213480)](https://www.abcam.com/ps/products/213/ab213480/Images/ab213480-6-benefits-of-recombinant-antibodies.png)
Anti-PD-L1 antibody [EPR20529] (ab213480)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (26)
ab213480 has been referenced in 26 publications.
- Fang W et al. Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8+ T cell exclusion. J Exp Clin Cancer Res 40:4 (2021). PubMed: 33390170
- Li H et al. The Beneficial Role of Sunitinib in Tumor Immune Surveillance by Regulating Tumor PD-L1. Adv Sci (Weinh) 8:2001596 (2021). PubMed: 33510997
- Liu X et al. Combination Chemo-Immunotherapy for Pancreatic Cancer Using the Immunogenic Effects of an Irinotecan Silicasome Nanocarrier Plus Anti-PD-1. Adv Sci (Weinh) 8:2002147 (2021). PubMed: 33747719
- Wang Z et al. Abrogation of USP7 is an alternative strategy to downregulate PD-L1 and sensitize gastric cancer cells to T cells killing. Acta Pharm Sin B 11:694-707 (2021). PubMed: 33777676
- Yang J et al. Circular RNA CHST15 Sponges miR-155-5p and miR-194-5p to Promote the Immune Escape of Lung Cancer Cells Mediated by PD-L1. Front Oncol 11:595609 (2021). PubMed: 33777742