Anti-PD1 antibody (ab89828)
Key features and details
- Mouse polyclonal to PD1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PD1 antibody
See all PD1 primary antibodies -
Description
Mouse polyclonal to PD1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human PD1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human stomach tissue lysate; HeLa whole cell lysate (ab150035); PD1 transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. |
Target
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Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5133 Human
- Omim: 600244 Human
- SwissProt: Q15116 Human
- Unigene: 158297 Human
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Alternative names
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
see all
Images
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Western blot - Anti-PD1 antibody (ab89828)Anti-PD1 antibody (ab89828) at 1 µg/ml + human stomach tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
Western blot - Anti-PD1 antibody (ab89828)Anti-PD1 antibody (ab89828) at 1 µg/ml + HeLa cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: ~32 kDa why is the actual band size different from the predicted? -
Western blot - Anti-PD1 antibody (ab89828)All lanes : Anti-PD1 antibody (ab89828) at 1 µg/ml
Lane 1 : PD1 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: ~38 kDa, ~45 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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Datasheet download
References (5)
ab89828 has been referenced in 5 publications.
- Ishikawa R et al. Fusogenic Hybrid Extracellular Vesicles with PD-1 Membrane Proteins for the Cytosolic Delivery of Cargos. Cancers (Basel) 14:N/A (2022). PubMed: 35681615
- Zhang HY et al. Overexpressed Tumor Suppressor Exosomal miR-15a-5p in Cancer Cells Inhibits PD1 Expression in CD8+T Cells and Suppresses the Hepatocellular Carcinoma Progression. Front Oncol 11:622263 (2021). PubMed: 33816255
- Lai X & Friedman A How to schedule VEGF and PD-1 inhibitors in combination cancer therapy? BMC Syst Biol 13:30 (2019). PubMed: 30894166
- Lai X & Friedman A Combination therapy of cancer with cancer vaccine and immune checkpoint inhibitors: A mathematical model. PLoS One 12:e0178479 (2017). PubMed: 28542574
- Lai X & Friedman A Combination therapy for melanoma with BRAF/MEK inhibitor and immune checkpoint inhibitor: a mathematical model. BMC Syst Biol 11:70 (2017). PubMed: 28724377
