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  1. Link

    products/primary-antibodies/pd1-antibody-ab89828.pdf

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Cell Biology Apoptosis Receptors Death Receptors
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Anti-PD1 antibody (ab89828)

  • Datasheet
Submit a review Submit a question References (5)

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Western blot - Anti-PD1 antibody (ab89828)
  • Western blot - Anti-PD1 antibody (ab89828)
  • Western blot - Anti-PD1 antibody (ab89828)

Key features and details

  • Mouse polyclonal to PD1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-PD1 antibody [CAL15] (ab237727)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-PD1 antibody
    See all PD1 primary antibodies
  • Description

    Mouse polyclonal to PD1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human PD1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

  • Positive control

    • Human stomach tissue lysate; HeLa whole cell lysate (ab150035); PD1 transfected 293T cell lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Death Receptors
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Cancer
    • Tumor immunology
    • CD markers
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Cancer
    • Cell Death
    • Apoptosis
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)
  • Recombinant Protein

    • Recombinant human PD1 protein (Active) (ab174035)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab89828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.

Target

  • Function

    Possible cell death inducer, in association with other factors.
  • Involvement in disease

    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage

    Induced at programmed cell death.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q15116 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5133 Human
    • Omim: 600244 Human
    • SwissProt: Q15116 Human
    • Unigene: 158297 Human
    • Alternative names

      • CD279 antibody
      • CD279 antigen antibody
      • hPD 1 antibody
      • hPD l antibody
      • hPD-1 antibody
      • hSLE1 antibody
      • PD 1 antibody
      • PD-1 antibody
      • PD1 antibody
      • PDCD 1 antibody
      • PDCD1 antibody
      • PDCD1_HUMAN antibody
      • Programmed cell death 1 antibody
      • Programmed cell death 1 protein antibody
      • Programmed cell death protein 1 antibody
      • Protein PD 1 antibody
      • Protein PD-1 antibody
      • SLEB2 antibody
      • Systemic lupus erythematosus susceptibility 2 antibody
      see all

    Images

    • Western blot - Anti-PD1 antibody (ab89828)
      Western blot - Anti-PD1 antibody (ab89828)
      Anti-PD1 antibody (ab89828) at 1 µg/ml + human stomach tissue lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 32 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-PD1 antibody (ab89828)
      Western blot - Anti-PD1 antibody (ab89828)
      Anti-PD1 antibody (ab89828) at 1 µg/ml + HeLa cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 32 kDa
      Observed band size: ~32 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-PD1 antibody (ab89828)
      Western blot - Anti-PD1 antibody (ab89828)
      All lanes : Anti-PD1 antibody (ab89828) at 1 µg/ml

      Lane 1 : PD1 transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 32 kDa
      Observed band size: 32 kDa
      Additional bands at: ~38 kDa, ~45 kDa. We are unsure as to the identity of these extra bands.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (5)

    Publishing research using ab89828? Please let us know so that we can cite the reference in this datasheet.

    ab89828 has been referenced in 5 publications.

    • Ishikawa R  et al. Fusogenic Hybrid Extracellular Vesicles with PD-1 Membrane Proteins for the Cytosolic Delivery of Cargos. Cancers (Basel) 14:N/A (2022). PubMed: 35681615
    • Zhang HY  et al. Overexpressed Tumor Suppressor Exosomal miR-15a-5p in Cancer Cells Inhibits PD1 Expression in CD8+T Cells and Suppresses the Hepatocellular Carcinoma Progression. Front Oncol 11:622263 (2021). PubMed: 33816255
    • Lai X & Friedman A How to schedule VEGF and PD-1 inhibitors in combination cancer therapy? BMC Syst Biol 13:30 (2019). PubMed: 30894166
    • Lai X & Friedman A Combination therapy of cancer with cancer vaccine and immune checkpoint inhibitors: A mathematical model. PLoS One 12:e0178479 (2017). PubMed: 28542574
    • Lai X & Friedman A Combination therapy for melanoma with BRAF/MEK inhibitor and immune checkpoint inhibitor: a mathematical model. BMC Syst Biol 11:70 (2017). PubMed: 28724377

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