Recombinant Anti-PD1 antibody [CAL15] (ab237727)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)](https://www.abcam.com/ps/products/237/ab237727/Images/ab237727-349380-anti-pd1-antibody-cal15-immunohistochemistry-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAL15] to PD1
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PD1 antibody [CAL15]
See all PD1 primary antibodies -
Description
Rabbit monoclonal [CAL15] to PD1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MOLT-4 (treated with PMA (10ng/ml 24h) and Ionomycin (500ng/ml 24h) whole cell lysate; human tonsil and thymus lysates. IHC-P: Human tonsil and spleen tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity >99% -
Clonality
Monoclonal -
Clone number
CAL15 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/1000. Detects a band of approximately 50-55 kDa (predicted molecular weight: 32 kDa).
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| Notes |
|---|
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000. Detects a band of approximately 50-55 kDa (predicted molecular weight: 32 kDa). |
Target
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Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5133 Human
- Omim: 600244 Human
- SwissProt: Q15116 Human
- Unigene: 158297 Human
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Alternative names
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab237727 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human tonsil is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab237727 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)](https://www.abcam.com/ps/products/237/ab237727/Images/ab237727-349381-anti-pd1-antibody-cal15-immunohistochemistry-spleen-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling PD1 with ab237727 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human spleen is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab237727 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Western blot - Anti-PD1 antibody [CAL15] (ab237727)](https://www.abcam.com/ps/products/237/ab237727/Images/ab237727-349382-anti-pd1-antibody-cal15-western-blot-human.jpg)
Western blot - Anti-PD1 antibody [CAL15] (ab237727)All lanes : Anti-PD1 antibody [CAL15] (ab237727) at 1/1000 dilution
Lane 1 : Untreated MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 2 : MOLT-4 (treated with PMA (10ng/ml 24h) and Ionomycin (500ng/ml 24h) whole cell lysate
Lane 3 : Human tonsil lysate
Lane 4 : Human thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 32 kDa
Observed band size: 50-55 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
The expression profile observed is consistent with what has been described in the literature (PMID: 26318293). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)](https://www.abcam.com/ps/products/237/ab237727/Images/ab237727-315295-anti-pd1-antibody-cal15-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL15] (ab237727)Formalin-fixed, paraffin-embedded human tonsil tissue stained for PD1 using ab237727 at 0.25 µg/ml dilution in immunohistochemical analysis.
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![Anti-PD1 antibody [CAL15] (ab237727)](https://www.abcam.com/ps/products/237/ab237727/Images/ab237727-5-benefits-of-recombinant-antibodies.png)
Anti-PD1 antibody [CAL15] (ab237727)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab237727 has been referenced in 1 publication.
- Bahnassy AA et al. The prognostic role of PD-1, PD-L1, ALK, and ROS1 proteins expression in non-small cell lung carcinoma patients from Egypt. J Egypt Natl Canc Inst 34:23 (2022). PubMed: 35644823
