Recombinant Anti-PD1 antibody [NAT105] (ab234444)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [NAT105] to PD1
- Suitable for: Flow Cyt, Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PD1 antibody [NAT105]
See all PD1 primary antibodies -
Description
Mouse monoclonal [NAT105] to PD1 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MOLT-4 treated with 500 ng/ml ionomycin and 10 ng/ml phorbol-12-myristate-13-acetate (PMA) for 24 hours, whole cell lysate. IHC-P: Human tonsil tissue. Flow Cyt (intra): MOLT-4 cells treated with ionomycin (500 ng/ml, 24h) and PMA (10 ng/ml, 24h). ICC/IF: MOLT-4 cells treated with Ionomycin (500ng/ml, 24 h) and PMA (10ng/ml, 24 h) Flow cyto: Human PBMCs
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
NAT105 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)
- Alexa Fluor® 488 Anti-PD1 antibody [NAT105] (ab220300)
- Alexa Fluor® 647 Anti-PD1 antibody [NAT105] (ab220301)
- PE Anti-PD1 antibody [NAT105] (ab220302)
- Alexa Fluor® 647 Anti-PD1 antibody [NAT105] (ab279695)
- Alexa Fluor® 555 Anti-PD1 antibody [NAT105] (ab280864)
- Anti-PD1 antibody [NAT105] (ab52587)
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
1/100.
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ICC/IF |
1/50.
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WB |
1/1000. Use under non reducing condition. Predicted molecular weight: 32 kDa.
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IHC-P |
1/50.
Perform heat-mediated antigen retrieval using Sodium citrate buffer (pH 6.0), 20 mins. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. |
Flow Cyt (Intra)
1/100. |
ICC/IF
1/50. |
WB
1/1000. Use under non reducing condition. Predicted molecular weight: 32 kDa. |
IHC-P
1/50. Perform heat-mediated antigen retrieval using Sodium citrate buffer (pH 6.0), 20 mins. |
Target
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Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5133 Human
- Omim: 600244 Human
- SwissProt: Q15116 Human
- Unigene: 158297 Human
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Alternative names
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
see all
Images
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Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab234444 (right) or Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab234444 or Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (1x 106 in 100 µl at 5.0 μg/ml (1/516)) for 30min on ice. The cells were simultaneously stained with CD3.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab234444)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab234444 at 1/50 dilution, followed by Rabbit Anti-Mouse IgG + Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells of human tonsil germinal center is observed. Performed on a Leica Biosystems BOND instrument. Counter stained with hematoxylin.
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Ab234444 staining PD1 in the MOLT-4 (human lymphoblastic leukemia T lymphoblast) treated with Ionomycin (500 ng/ml, 24 h) and PMA (10 ng/ml, 24 h) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50). ab150117 anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed (1/100) was used as the secondary antibody.
ab179504 Anti-beta IV Tubulin (1/200) was used as a counter stain, detected by ab150080 AlexaFluor®594 Goat anti- Rabbit secondary (1/500)
DAPI was used as a Nuclear counter stain.
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Intracellular flow cytometric analysis ofMOLT-4 (human lymphoblastic leukemia cell line) cell line treated with ionomycin (500 ng/ml, 24h) and PMA (10 ng/ml, 24h)labeling PD1 with ab234444 at 1/1000 dilution (red) and an untreated control (green) compared with aMouse monoclonalIgG1 (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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All lanes : Anti-PD1 antibody [NAT105] (ab234444) at 1/1000 dilution
Lane 1 : Untreated MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 2 : MOLT-4 (human lymphoblastic leukemia cell line) treated with 500 ng/ml ionomycin and 10 ng/ml phorbol-12-myristate-13-acetate (PMA) for 24 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L) Peroxidase Peroxidase-Conjugated at 1/5000 dilution
Predicted band size: 32 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 26318293)
We recommend that the customer optimizes the blotting conditions for themselves.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab234444 has been referenced in 4 publications.
- Qiao T et al. Combined pembrolizumab and bevacizumab therapy effectively inhibits non-small-cell lung cancer growth and prevents postoperative recurrence and metastasis in humanized mouse model. Cancer Immunol Immunother 72:1169-1181 (2023). PubMed: 36357599
- Qiao T et al. Inhibition of LDH-A by Oxamate Enhances the Efficacy of Anti-PD-1 Treatment in an NSCLC Humanized Mouse Model. Front Oncol 11:632364 (2021). PubMed: 33859941
- Smolle MA et al. T-regulatory cells predict clinical outcome in soft tissue sarcoma patients: a clinico-pathological study. Br J Cancer 125:717-724 (2021). PubMed: 34127811
- Ji F et al. Tumor Microenvironment Characterization in Breast Cancer Identifies Prognostic and Neoadjuvant Chemotherapy Relevant Signatures. Front Mol Biosci 8:759495 (2021). PubMed: 34708079