Recombinant Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [NAT105] to PD1 - BSA and Azide free
- Suitable for: ICC/IF, WB, Flow Cyt (Intra), IP, IHC-Fr, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PD1 antibody [NAT105] - BSA and Azide free
See all PD1 primary antibodies -
Description
Mouse monoclonal [NAT105] to PD1 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, Flow Cyt (Intra), IP, IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human PD1. TY cells (human T/NK cell Leukemia).
Database link: Q15116 -
Positive control
- IHC-P: Human tonsil tissue. Flow cyto (intra): Human PBMCs
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General notes
ab201811 is the carrier-free version of ab52587.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
NAT105 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform heat-mediated antigen retrieval using Sodium citrate buffer (pH 6.0), 20 mins. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval using Sodium citrate buffer (pH 6.0), 20 mins. |
Target
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Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5133 Human
- Omim: 600244 Human
- SwissProt: Q15116 Human
- Unigene: 158297 Human
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Alternative names
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
see all
Images
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234444).
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab234444 (right) or Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab234444 or Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (1x 106 in 100 µl at 5.0 μg/ml (1/516)) for 30min on ice. The cells were simultaneously stained with CD3.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
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Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)
Ab234444 staining PD1 in the MOLT-4 (human lymphoblastic leukemia T lymphoblast) treated with Ionomycin (500 ng/ml, 24 h) and PMA (10 ng/ml, 24 h) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50). ab150117 anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed (1/100) was used as the secondary antibody.
ab179504 Anti-beta IV Tubulin (1/200) was used as a counter stain, detected by ab150080 AlexaFluor®594 Goat anti- Rabbit secondary (1/500)
DAPI was used as a Nuclear counter stain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234444).
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Intracellular flow cytometric analysis ofMOLT-4 (human lymphoblastic leukemia cell line) cell line treated with ionomycin (500 ng/ml, 24h) and PMA (10 ng/ml, 24h)labeling PD1 with ab234444 at 1/1000 dilution (red) and an untreated control (green) compared with aMouse monoclonalIgG1 (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG H&L (Alexa Fluorr® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234444).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab234444 at 1/50 dilution, followed by Rabbit Anti-Mouse IgG + Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells of human tonsil germinal center is observed. Performed on a Leica Biosystems BOND instrument. Counter stained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234444).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab201811 has been referenced in 1 publication.
- Lu Y et al. PD1+ tumor associated macrophages predict poor prognosis of locally advanced esophageal squamous cell carcinoma. Future Oncol 15:4019-4030 (2019). PubMed: 31612729