Recombinant Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)
![Immunoprecipitation - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)](https://www.abcam.com/ps/products/206/ab206378/Images/ab206378-309856-anti-pd1-phospho-y248-antibody-epr19821-immunoprecipitation.jpg "Immunoprecipitation - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19821] to PD1 (phospho Y248)
- Suitable for: WB, Dot blot, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PD1 (phospho Y248) antibody [EPR19821]
See all PD1 primary antibodies -
Description
Rabbit monoclonal [EPR19821] to PD1 (phospho Y248) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blot, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with a PD1 (WT) plus a 25 kDa fusion protein expression vector, then treated with 100 µM pervanadate for 10 minutes whole cell lysate. IP: HEK-293T transfected with PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 µM pervanadate for 10 minutes whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19821 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 32 kDa.
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| Dot blot |
1/1000.
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| IP |
1/30.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 32 kDa. |
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Dot blot
1/1000. |
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IP
1/30. |
Target
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Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5133 Human
- Omim: 600244 Human
- SwissProt: Q15116 Human
- Unigene: 158297 Human
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Alternative names
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
see all
Images
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Immunoprecipitation - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)
PD1 (phospho Y248) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate, with ab206378 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206378 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: HEK-293T transfected with a PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate 10 μg (Input).
Lane 2: ab206378 IP in HEK-293T transfected with a PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206378 in HEK-293T transfected with a PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate.
Exposure time: 30 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBSTThe observed bands around 75 kDa comprise PD-1 plus the 25 kDa fusion protein. The plasmids were kindly provided by our collaborator Dr. Liang Chen, NIBS.
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![Western blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)](https://www.abcam.com/ps/products/206/ab206378/Images/ab206378-309855-anti-pd1-phospho-y248-antibody-epr19821-western-blot.jpg)
Western blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)All lanes : Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378) at 1/1000 dilution
Lane 1 : Untreated-HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate transfected with a PD1 (WT) plus a 25 kDa fusion protein expression vector
Lane 2 : HEK-293T transfected with a PD1 (WT) plus a 25 kDa fusion protein expression vector, then treated with 100 µM pervanadate for 10 minutes whole cell lysate
Lane 3 : HEK-293T transfected with PD1 (WT) plus a 25 kDa fusion protein expression vector, then treated with 100 µM pervanadate for 10 minutes, whole cell lysate (20 µg). Following the transfer to PVDF, the membrane was treated with alkaline phosphatase for 1h at 37°C.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 70-75 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The observed bands at around 75 kDa are PD-1 plus the 25 kDa fusion protein. The expression profile observed is consistent with what has been described in the literature (PMID: 22641383). The plasmids were kindly provided by our collaborator Dr. Liang Chen NIBS.
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![Dot Blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)](https://www.abcam.com/ps/products/206/ab206378/Images/ab206378-309854-anti-pd1-phospho-y248-antibody-epr19821-dot-blot.jpg)
Dot Blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)Dot blot analysis of PD1 (phospho Y248) labeled with ab206378 at 1/1000 dilution.
Lane 1: PD1 (phospho Y248) peptide.
Lane 2: PD1 non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 1 minute.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
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![Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)](https://www.abcam.com/ps/products/206/ab206378/Images/ab206378-5-benefits-of-recombinant-antibodies.png)
Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab206378 has not yet been referenced specifically in any publications.