Recombinant Anti-PDGF AA antibody [EPR19924] (ab203911)
![Immunoprecipitation - Anti-PDGF AA antibody [EPR19924] (ab203911)](https://www.abcam.com/ps/products/203/ab203911/Images/ab203911-274006-anti-pdgf-aa-antibody-epr19924-immunoprecipitation.jpg "Immunoprecipitation - Anti-PDGF AA antibody [EPR19924] (ab203911)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19924] to PDGF AA
- Suitable for: IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PDGF AA antibody [EPR19924]
See all PDGF AA primary antibodies -
Description
Rabbit monoclonal [EPR19924] to PDGF AA -
Host species
Rabbit -
Specificity
This antibody does not react with human species in the Western Blot application.
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Tested applications
Suitable for: IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Human kidney lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19924 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
|
| Notes |
|---|
|
IP
1/30. |
Target
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Function
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Domain
The long form contains a basic insert which acts as a cell retention signal. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5154 Human
- Omim: 173430 Human
- SwissProt: P04085 Human
- Unigene: 535898 Human
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Alternative names
- PDGF A antibody
- PDGF A chain antibody
- PDGF subunit A antibody
see all
Images
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Immunoprecipitation - Anti-PDGF AA antibody [EPR19924] (ab203911)
PDGF AA was immunoprecipitated from 0.35 mg of Human kidney lysate with ab203911 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203911 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human kidney lysate, 10 μg (Input).
Lane 2: ab203911 IP in Human kidney lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203911 in Human kidney lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Anti-PDGF AA antibody [EPR19924] (ab203911)](https://www.abcam.com/ps/products/203/ab203911/Images/ab203911-5-benefits-of-recombinant-antibodies.png)
Anti-PDGF AA antibody [EPR19924] (ab203911)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab203911 has been referenced in 2 publications.
- Guo J et al. Circular Ribonucleic Acid circFTO Promotes Angiogenesis and Impairs Blood-Retinal Barrier Via Targeting the miR-128-3p/Thioredoxin Interacting Protein Axis in Diabetic Retinopathy. Front Mol Biosci 8:685466 (2021). PubMed: 34422901
- Xie Y et al. miR-363 suppresses the proliferation, migration and invasion of clear cell renal cell carcinoma by downregulating S1PR1. Cancer Cell Int 20:227 (2020). PubMed: 32536815