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    products/primary-antibodies/pdgf-aa-antibody-epr19924-ab203911.pdf

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RecombinantRabMAb

Recombinant Anti-PDGF AA antibody [EPR19924] (ab203911)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Immunoprecipitation - Anti-PDGF AA antibody [EPR19924] (ab203911)
  • Anti-PDGF AA antibody [EPR19924] (ab203911)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19924] to PDGF AA
  • Suitable for: IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Preparation
Extraction Buffer 5X PTR (ab193970)
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Recombinant human Interferon alpha 2 protein (Active) (ab200262)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-PDGF AA antibody [EPR19924]
    See all PDGF AA primary antibodies
  • Description

    Rabbit monoclonal [EPR19924] to PDGF AA
  • Host species

    Rabbit
  • Specificity

    This antibody does not react with human species in the Western Blot application.

  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Human kidney lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19924
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Growth Factors/Hormones
    • PDGF
    • Immunology
    • Secreted Molecules
    • Other secreted molecules
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Alternative Versions

    • Anti-PDGF AA antibody [EPR19924] - BSA and Azide free (ab251407)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant human PDGF AA protein (ab9702)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab203911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/30.
Notes
IP
1/30.
Application notes
Is unsuitable for WB.

Target

  • Function

    Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Domain

    The long form contains a basic insert which acts as a cell retention signal.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P04085 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5154 Human
    • Omim: 173430 Human
    • SwissProt: P04085 Human
    • Unigene: 535898 Human
    • Alternative names

      • PDGF A antibody
      • PDGF A chain antibody
      • PDGF subunit A antibody
      • PDGF-1 antibody
      • PDGF1 antibody
      • PDGFA antibody
      • PDGFA_HUMAN antibody
      • Platelet derived growth factor alpha chain antibody
      • Platelet derived growth factor alpha isoform 2 preproprotein antibody
      • Platelet derived growth factor alpha polypeptide antibody
      • Platelet-derived growth factor A chain antibody
      • Platelet-derived growth factor alpha polypeptide antibody
      • Platelet-derived growth factor subunit A antibody
      see all

    Images

    • Immunoprecipitation - Anti-PDGF AA antibody [EPR19924] (ab203911)
      Immunoprecipitation - Anti-PDGF AA antibody [EPR19924] (ab203911)

      PDGF AA was immunoprecipitated from 0.35 mg of Human kidney lysate with ab203911 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203911 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: Human kidney lysate, 10 μg (Input).

      Lane 2: ab203911 IP in Human kidney lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203911 in Human kidney lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 seconds.

       

    • Anti-PDGF AA antibody [EPR19924] (ab203911)
      Anti-PDGF AA antibody [EPR19924] (ab203911)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab203911? Please let us know so that we can cite the reference in this datasheet.

    ab203911 has been referenced in 1 publication.

    • Xie Y  et al. miR-363 suppresses the proliferation, migration and invasion of clear cell renal cell carcinoma by downregulating S1PR1. Cancer Cell Int 20:227 (2020). PubMed: 32536815

    Customer reviews and Q&As

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