Recombinant Anti-PDGF B antibody [326A] (ab107101)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] (ab107101)](https://www.abcam.com/ps/products/107/ab107101/Images/ab107101-392158-AntiPDGF-B-antibody-326A-immunohistochemistry-glioma-human1.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] (ab107101)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [326A] to PDGF B
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PDGF B antibody [326A]
See all PDGF B primary antibodies -
Description
Rat monoclonal [326A] to PDGF B -
Host species
Rat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human PDGF B. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P01127 -
Positive control
- IHC-P: Human glioma, kidney and cerebrum tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
326A -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab107101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 2.56 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
Use a concentration of 2.56 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 5155 Human
- Omim: 190040 Human
- SwissProt: P01127 Human
- Unigene: 1976 Human
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Alternative names
- Becaplermin antibody
- c sis antibody
- FLJ12858 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] (ab107101)
Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling PDGF B with ab107101 at 2.56 µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human glioma. The section was incubated with ab107101 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] (ab107101)](https://www.abcam.com/ps/products/107/ab107101/Images/ab107101-392157-AntiPDGF-B-antibody-326A-immunohistochemistry-kidney-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] (ab107101)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling PDGF B with ab107101 at 2.56 µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on glomerus of human kidney. The section was incubated with ab107101 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] (ab107101)](https://www.abcam.com/ps/products/107/ab107101/Images/ab107101-392155-AntiPDGF-B-antibody-326A-immunohistochemistry-cerebrum-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] (ab107101)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling PDGF B with ab107101 at 2.56 µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human cerebrum. The section was incubated with ab107101 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Anti-PDGF B antibody [326A] (ab107101)](https://www.abcam.com/ps/products/107/ab107101/Images/ab107101-5-benefits-of-recombinant-antibodies.png)
Anti-PDGF B antibody [326A] (ab107101)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab107101 has not yet been referenced specifically in any publications.