Recombinant Anti-PDGF B antibody [EPR6834] (ab178409)
![Western blot - Anti-PDGF B antibody [EPR6834] (ab178409)](https://www.abcam.com/ps/products/178/ab178409/Images/ab178409-383291-anti-pdgf-b-antibody-epr6834-westernblot-human-mouse-rat.jpg "Western blot - Anti-PDGF B antibody [EPR6834] (ab178409)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6834] to PDGF B
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-PDGF B antibody [EPR6834]
See all PDGF B primary antibodies -
Description
Rabbit monoclonal [EPR6834] to PDGF B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep
-
Immunogen
Synthetic peptide within Human PDGF B aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P01127 -
Positive control
- WB: Fetal brain tissue lysate, Human, Mouse and Rat brain tissue lysates, Mouse and Rat heart tissue lysates; A375 and HeLa cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6834 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
Target
- Information by UniProt
-
Database links
- Entrez Gene: 5155 Human
- Entrez Gene: 18591 Mouse
- Entrez Gene: 24628 Rat
- Entrez Gene: 443545 Sheep
- Omim: 190040 Human
- SwissProt: P01127 Human
- SwissProt: P31240 Mouse
- SwissProt: Q05028 Rat
see all -
Alternative names
- Becaplermin antibody
- c sis antibody
- FLJ12858 antibody
see all
Images
-
Western blot - Anti-PDGF B antibody [EPR6834] (ab178409)All lanes : Anti-PDGF B antibody [EPR6834] (ab178409) at 1/1000 dilution (Purified)
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Human brain tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
-
![Western blot - Anti-PDGF B antibody [EPR6834] (ab178409)](https://www.abcam.com/ps/products/178/ab178409/Images/ab178409-206274-anti-pdgf-b-antibody-epr6834-western-blot.jpg)
Western blot - Anti-PDGF B antibody [EPR6834] (ab178409)All lanes : Anti-PDGF B antibody [EPR6834] (ab178409) at 1/1000 dilution (unpurified)
Lane 1 : Fetal brain tissue lysate
Lane 2 : A375 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa -
![Anti-PDGF B antibody [EPR6834] (ab178409)](https://www.abcam.com/ps/products/178/ab178409/Images/ab178409-6-benefits-of-recombinant-antibodies.png)
Anti-PDGF B antibody [EPR6834] (ab178409)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (15)
ab178409 has been referenced in 15 publications.
- Li L et al. MiR-325-3p mediate the CXCL17/CXCR8 axis to regulate angiogenesis in hepatocellular carcinoma. Cytokine 141:155436 (2021). PubMed: 33515898
- Duan YY et al. Microtubule Stabilization Promotes Microcirculation Reconstruction After Spinal Cord Injury. J Mol Neurosci 71:583-595 (2021). PubMed: 32901373
- Santhanam L et al. Skeleton-secreted PDGF-BB mediates arterial stiffening. J Clin Invest 131:N/A (2021). PubMed: 34437300
- Suehara Y et al. Assessment of Predictive Biomarkers of the Response to Pazopanib Based on an Integrative Analysis of High-grade Soft-tissue Sarcomas: Analysis of a Tumor Sample from a Responder and Patients with Other Soft-tissue Sarcomas. Clin Orthop Relat Res 478:2461-2476 (2020). PubMed: 32567826
- Mao Y et al. Fibroblast growth factor-2/platelet-derived growth factor enhances atherosclerotic plaque stability. J Cell Mol Med 24:1128-1140 (2020). PubMed: 31755222