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    products/primary-antibodies/pdgf-b-antibody-epr6834-bsa-and-azide-free-ab240207.pdf

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RecombinantRabMAb

Recombinant Anti-PDGF B antibody [EPR6834] - BSA and Azide free (ab240207)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-PDGF B antibody [EPR6834] - BSA and Azide free (ab240207)
  • Western blot - Anti-PDGF B antibody [EPR6834] - BSA and Azide free (ab240207)
  • Anti-PDGF B antibody [EPR6834] - BSA and Azide free (ab240207)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6834] to PDGF B - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)
Primary
Product image
Anti-PDGF B antibody [EPR6834] (ab178409)
Protein
Product image
Recombinant human PDGF B protein (Active) (ab259425)

View more associated products

Overview

  • Product name

    Anti-PDGF B antibody [EPR6834] - BSA and Azide free
    See all PDGF B primary antibodies
  • Description

    Rabbit monoclonal [EPR6834] to PDGF B - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240207 is the carrier-free version of ab178409.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6834
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Signal Transduction
    • Growth Factors/Hormones
    • PDGF
    • Cancer
    • Growth factors
    • PDGF
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Developmental Biology
    • Organogenesis
    • Angiogenesis and vasculogenesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-PDGF B antibody [EPR6834] (ab178409)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Immunohistochemistry kits

    • Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant human PDGF B protein (Active) (ab259425)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab240207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
Application notes
Is unsuitable for IHC-P.

Target

  • Target information above from: UniProt accession P01127 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5155 Human
    • Entrez Gene: 18591 Mouse
    • Entrez Gene: 24628 Rat
    • Entrez Gene: 443545 Sheep
    • Omim: 190040 Human
    • SwissProt: P01127 Human
    • SwissProt: P31240 Mouse
    • SwissProt: Q05028 Rat
    • SwissProt: Q95229 Sheep
    • Unigene: 1976 Human
    • Unigene: 144089 Mouse
    • Unigene: 198230 Rat
    see all
  • Alternative names

    • Becaplermin antibody
    • c sis antibody
    • FLJ12858 antibody
    • Oncogene SIS antibody
    • PDGF 2 antibody
    • PDGF B chain antibody
    • PDGF Beta antibody
    • PDGF subunit B antibody
    • PDGF-2 antibody
    • PDGF2 antibody
    • Pdgfb antibody
    • PDGFB_HUMAN antibody
    • Platelet derived growth factor 2 antibody
    • Platelet derived growth factor B chain antibody
    • Platelet derived growth factor beta antibody
    • Platelet derived growth factor beta polypeptide (oncogene SIS) antibody
    • Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog) antibody
    • Platelet derived growth factor beta polypeptide antibody
    • Platelet-derived growth factor B chain antibody
    • Platelet-derived growth factor beta polypeptide antibody
    • Platelet-derived growth factor subunit B antibody
    • Proto-oncogene c-Sis antibody
    • Simian sarcoma viral (v sis) oncogene homolog antibody
    • SIS antibody
    • SSV antibody
    • v sis platelet derived growth factor beta polypeptide antibody
    see all

Images

  • Western blot - Anti-PDGF B antibody [EPR6834] - BSA and Azide free (ab240207)
    Western blot - Anti-PDGF B antibody [EPR6834] - BSA and Azide free (ab240207)
    All lanes : Anti-PDGF B antibody [EPR6834] (ab178409) at 1/1000 dilution (Purified)

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate
    Lane 3 : Human brain tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa



    This data was developed using ab178409, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PDGF B antibody [EPR6834] - BSA and Azide free (ab240207)
    Western blot - Anti-PDGF B antibody [EPR6834] - BSA and Azide free (ab240207)
    All lanes : Anti-PDGF B antibody [EPR6834] (ab178409) at 1/1000 dilution (unpurified)

    Lane 1 : Fetal brain tissue lysate
    Lane 2 : A375 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 27 kDa



    This data was developed using ab178409, the same antibody clone in a different buffer formulation.

  • Anti-PDGF B antibody [EPR6834] - BSA and Azide free (ab240207)
    Anti-PDGF B antibody [EPR6834] - BSA and Azide free (ab240207)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab240207? Please let us know so that we can cite the reference in this datasheet.

ab240207 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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